Effects of capsaicin on visceral smooth muscle: a valuable tool for sensory neurotransmitter identification

Barthó, L.; Benkó, Rita; Patacchini, Riccardo; Pethő, Gábor; Holzer‐Petsche, Ulrike; Holzer, Peter; Lázár, Zsófia; Undi, Sarolta; Illényi, L; Antal, A; Horváth, Örs Péter

doi:10.1016/j.ejphar.2004.07.020

Cited by 59 publications

(77 citation statements)

References 154 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Capsaicin has been thought to deplete the neuropeptides not only from the sensory nerve fibres (38,39), but also from the airway epithelial cells (34). Our present findings that the depletion of neuropeptides by capsaicin pretreatment increases the adenosineinduced relaxation and that the liberation of neuropeptides in the presence of capsaicin inhibits the action of the purine nucleoside provided pharmacological proof that continuous treatment with caffeine leads to an adaptive response of the tracheal epithelial cells that counterbalances the actions of adenosine.…”

Section: Discussionsupporting

confidence: 61%

Modulation of Adenosine-Induced Responses in the Guinea-Pig Trachea During Long-Term Caffeine Treatment: Possible Role of Epithelium

et al. 2007

View full text Add to dashboard Cite

Abstract. The responses to adenosine were studied on isolated, methacholine-precontracted tracheal strips of guinea pigs in the course of long-term caffeine or solvent treatment. Guinea pigs were fed caffeine for 10 weeks (average serum caffeine concentration: 39.1 ± 3.9 µM). In epithelium-intact tracheal preparations (EITPs), sensititization to adenosine-induced relaxation (AIR) developed. It attained a maximum in week 1 of caffeine treatment, and then its level diminished and disappeared completely by weeks 4 -6. In epithelium-denuded tracheal preparations (EDTPs), an increase in the sensitivity to adenosine was observed from week 1 to week 10 (a 4 -6-fold reduction in EC 50 ). Use of a coaxial bioassay system confirmed the role of epithelium in this process. The enhancement of the AIR of the EITPs was not modified by inhibitors of cyclooxygenase and lipoxygenase. Following depletion of the neuropeptides by acute capsaicin pretreatment, the AIR of the EITPs was strongly enhanced after caffeine treatment for 6 weeks. In chronically caffeine-treated EITPs, the inhibition of neutral endopeptidase led to dramatic reduction of the AIR. On the basis of the results by inhibiting nitric oxide synthase, it can be supposed that nitric oxide released from EITPs of long-lasting caffeine-treated animals operated as a constrictor agent. Our results show that chronic caffeine treatment gives rise to an initial sensitization to adenosine of the EITPs, this being followed by the development of a specific adaptive process in the epithelial cells, which counterbalances the increased tracheal sensitivity to adenosine.

show abstract

Section: Discussionsupporting

confidence: 61%

Modulation of Adenosine-Induced Responses in the Guinea-Pig Trachea During Long-Term Caffeine Treatment: Possible Role of Epithelium

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Results obtained from human tissues strongly differ from those obtained from animal preparations (Barthó et al, 2004). For example, Hammer et al, (1998) stated that the ability of intrajejunal capsaicin to give rise to pain was unexpected, because exposure of the rat gastric mucosa to 5-fold higher concentrations of capsaicin failed to evoke pseudoaffective reactions indicative of pain.…”

Section: E Human Visceral Studiesmentioning

confidence: 89%

Unravelling the Mystery of Capsaicin: A Tool to Understand and Treat Pain

O’Neill

Brock²,

Olesen³

et al. 2012

Pharmacol Rev

283

244

View full text Add to dashboard Cite

“…First, SP was reported to be degraded by ␣-chymotrypsin (22). Second, capsaicin was reported to deplete functional pools of SP (1,29). Thus it appears that the responses mediated by endogenous SP disappear.…”

Section: Involvement Of M Receptor In Efs-induced Sustained Contractionmentioning

confidence: 99%

“…Treatment of capsaicin also depletes some neurotransmitters other than SP reserved in enteric neurons (1). ATP, calcitonin gene-related peptide, and glutamate are suggested as candidates depleted by capsaicin.…”

Section: Involvement Of M Receptor In Efs-induced Sustained Contractionmentioning

confidence: 99%

M₂ and M₃ muscarinic receptors are involved in enteric nerve-mediated contraction of the mouse ileum: findings obtained with muscarinic-receptor knockout mouse

Takeuchi

Tanaka²,

Nakajima³

et al. 2007

American Journal of Physiology-Gastrointestinal and Liver Physi

View full text Add to dashboard Cite

Takeuchi T, Tanaka K, Nakajima H, Matsui M, Azuma Y-T. M 2 and M3 muscarinic receptors are involved in enteric nervemediated contraction of the mouse ileum: findings obtained with muscarinic-receptor knockout mouse. Am J Physiol Gastrointest Liver Physiol 292: G154 -G164, 2007; doi:10.1152/ajpgi.00173.2006.-The involvement of muscarinic receptors in neurogenic responses of the ileum was studied in wild-type and muscarinic-receptor (Mreceptor) knockout (KO) mice. Electrical field stimulation to the wild-type mouse ileum induced a biphasic response, a phasic and sustained contraction that was abolished by tetrodotoxin. The sustained contraction was prolonged for an extended period after the termination of electrical field stimulation. The phasic contraction was completely inhibited by atropine. In contrast, the sustained contraction was enhanced by atropine. Ileal strips prepared from M2-receptor KO mice exhibited a phasic contraction similar to that seen in wild-type mice and a sustained contraction that was larger than that in wild-type mice. In M3-receptor KO mice, the phasic contraction was smaller than that observed in wild-type mice. Acetylcholine exogenously administrated induced concentration-dependent contractions in strips isolated from wild-type, M2-and M3-receptor KO mice. However, contractions in M3-receptor KO mice shifted to the right. The sustained contraction was inhibited by capsaicin and neurokinin NK2 receptor antagonist, suggesting that it is mediated by substance P (SP). SP-induced contraction of M2-receptor KO mice did not differ from that of wild-type mice. SP immunoreactivity was located in enteric neurons, colocalized with M 2 receptor immunoreactivity. These results suggest that atropine-sensitive phasic contraction is mainly mediated via the M 3 receptor, and SP-mediated sustained contraction is negatively regulated by the M2 receptor at a presynaptic level.M2-and M3-receptor knockout mouse; neurogenic response; acetylcholine-mediated phasic contraction; substance P-mediated tonic contraction ACETYLCHOLINE (ACh) is released from cholinergic nervous terminals in the myenteric plexus, resulting in the contraction of intestinal smooth muscle through activation of muscarinic (M) receptors (9). Five subtypes of M receptors have so far been identified (39). It is well known that M 2 and M 3 receptors are present on the smooth muscle cells of gastrointestinal tracts and are expressed at ratios of 70 -80% and 20 -30%, respectively (6). A role of both M receptors in smooth muscle contraction was indicated by the examination of the effects of M-receptor subtype-specific antagonists on the contraction induced by exogenous M-receptor agonists (7). In the guinea pig and canine ileum, contractions induced by various Mreceptor agonists (carbachol, muscarine, and methacholine) were most strongly inhibited by the M 3 -receptor antagonist 4-diphenylacetoxy-N-methyl-piperidine methiodide (12, 27). In contrast, activation of the M 2 receptor in rat and guinea pig ileum was reported to inhibit isoproterenol...

show abstract

Effects of capsaicin on visceral smooth muscle: a valuable tool for sensory neurotransmitter identification

Cited by 59 publications

References 154 publications

Modulation of Adenosine-Induced Responses in the Guinea-Pig Trachea During Long-Term Caffeine Treatment: Possible Role of Epithelium

Modulation of Adenosine-Induced Responses in the Guinea-Pig Trachea During Long-Term Caffeine Treatment: Possible Role of Epithelium

Unravelling the Mystery of Capsaicin: A Tool to Understand and Treat Pain

M₂ and M₃ muscarinic receptors are involved in enteric nerve-mediated contraction of the mouse ileum: findings obtained with muscarinic-receptor knockout mouse

Contact Info

Product

Resources

About

Effects of capsaicin on visceral smooth muscle: a valuable tool for sensory neurotransmitter identification

Cited by 59 publications

References 154 publications

Modulation of Adenosine-Induced Responses in the Guinea-Pig Trachea During Long-Term Caffeine Treatment: Possible Role of Epithelium

Modulation of Adenosine-Induced Responses in the Guinea-Pig Trachea During Long-Term Caffeine Treatment: Possible Role of Epithelium

Unravelling the Mystery of Capsaicin: A Tool to Understand and Treat Pain

M2 and M3 muscarinic receptors are involved in enteric nerve-mediated contraction of the mouse ileum: findings obtained with muscarinic-receptor knockout mouse

Contact Info

Product

Resources

About

M₂ and M₃ muscarinic receptors are involved in enteric nerve-mediated contraction of the mouse ileum: findings obtained with muscarinic-receptor knockout mouse