Effects of calorie restriction on the expression of manganese superoxide dismutase and catalase under oxidative stress conditions in the rotifer Brachionus plicatilis

Abstract

Calorie restriction (CR) in the rotifer Brachionus plicatilis extends its lifespan, as it enhances the expression of antioxidant enzymes such as manganese superoxide dismutase (Mn SOD) and catalase. Here we show that CR also increased the mRNA levels of these antioxidant enzymes upon exposure to oxidative stress. Rotifers cultured under CR showed a higher survival rate than those fed ad libitum (AL) upon exposure to 0.05-0.2 lM juglone, an oxidative stress inducer. The relative mRNA levels of Mn SOD and catalase before exposure to juglone were slightly higher in the CR rotifers than in their AL counterparts, although these differences were not statistically significant. AL rotifers showed no apparent upregulation of the mRNA levels of these antioxidant enzymes upon exposure to 0.025 and 0.05 lM juglone. In contrast, the CR rotifers increased the mRNA levels of Mn SOD and catalase by up to 5.4-fold and 4.2-fold, respectively, resulting in significant differences between their levels in AL and CR rotifers under oxidative stress conditions. Furthermore, the protein level of catalase was clearly higher in CR than in AL rotifers 6 h after exposure to oxidative stress. These results suggest that the upregulation of Mn SOD and catalase genes is involved in CR-induced resistance to oxidative stress in the rotifer.

Changes in the expression of four heat shock proteins during the aging process in Brachionus calyciflorus (rotifera)

“…In fact, overexpression of hsp22 has been shown to increase fly lifespan, protect flies against paraquat, and improve their locomotor ability (Morrow et al 2004). In rotifers, DR pretreatment was reported to benefit paraquat and juglone tolerance (Kailasam et al 2011;Ozaki et al 2010). A previous study indicated that the beneficial effects of DR may contribute to the improvement in antioxidant gene expression (SOD and CAT) (Yang et al 2013).…”

Effect of copper exposure on GST activity and on the expression of four GSTs under oxidative stress condition in the monogonont rotifer, Brachionus koreanus

“…after 24 h exposure (Kailasam et al, 2011;Kim et al, 2011;Rhee et al, 2012). The test was conducted in triplicate and the results were presented as lethal concentrations (LC 10 and LC 50 ) observing 10 and 50% of mortality, respectively (Finney, 1971).…”

Changes in Expression of Manganese Superoxide Dismutase, Copper and Zinc Superoxide Dismutase and Catalase in Brachionus calyciflorus during the Aging Process

“…In the laboratory, DR is one of the most commonly used life-history alterations among evolutionarily distinct eukaryotes, from single-cell to multicellular organisms [16]–[19]. Previous studies have suggested that DR can extend the lifespan of rotifers and enhance their resistance to oxidative stress [20]–[24]. Interestingly, DR-induced longevity and other beneficial effects of DR can transmit to the next generation in rotifers [25].…”