Effects of calcium on development of anaerobic acidogenic biofilms

Huang, Jun; Pinder, K. L.

doi:10.1002/bit.260450305

Cited by 54 publications

(32 citation statements)

References 11 publications

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“…Hence, analysis of the removal efficiency of Ca 2+ under different aeration intensities, as well as the relevant influencing factors, was important for estimating the negative impact of Ca 2+ on the experiment. As shown in Table 2, despite the high concentration of Ca 2+ in the papermaking wastewater, the biofilm treatment method demonstrated great removal performances for both the large and small aeration intensities, similar to the results of Huang and Pinder (1995). During the formation process of the biofilms, aggregated microbes generate EPS that has the capability of adsorbing and combining part of Ca 2+ onto the biofilms, which can help to construct a more stable biofilm structure with a stronger ability of adjusting to the outer environment (Arp et al 2001).…”

Section: +supporting

confidence: 77%

Study on the Mass Transfer Enhancement in Biofilms Applied in Papermaking Wastewater Treatment

et al. 2017

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The research and refinement of papermaking wastewater treatment and reuse technology are important measures for energy conservation and emission reduction in the papermaking industry. This paper studied the process of biofilm formation and dissolved oxygen mass transfer of biofilms cultivated under different aeration intensities and attempted to enhance the biofilm reactor performance. The removal efficiencies of the chemical oxygen demand, total nitrogen, and ammonia nitrogen through biofilm treatment in two parallel biofilm reactors were higher under the larger aeration intensity (8 L/min) than under the smaller intensity (4 L/min). Macroscopically, this reflected the effect of dissolved oxygen on nitrogen removal. Microscopically, in terms of the dissolved oxygen profiles inside of the biofilms determined using a microelectrode probe, both aerobic and anaerobic layers occurred inside the biofilms, which suggested that simultaneous nitrification and denitrification occurred. The different aeration intensities led to differences in the internal and external dissolved oxygen concentrations in the biofilms, which affected the biofilm growth. This led to different micro-structures, and so the internal metabolism and wastewater treatment performance of the biofilms were not identical.

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Section: +supporting

confidence: 77%

Study on the Mass Transfer Enhancement in Biofilms Applied in Papermaking Wastewater Treatment

et al. 2017

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“…The authors hypothesized that calcium was important for stabilizing the biofilm matrix (9). Other studies have also suggested a role for calcium in stabilizing biofilms of bacteria (18,22,39).…”

Section: Discussionmentioning

confidence: 99%

Chelator-Induced Dispersal and Killing of Pseudomonas aeruginosa Cells in a Biofilm

Banin

Brady

Greenberg

2006

Appl Environ Microbiol

435

379

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Biofilms consist of groups of bacteria attached to surfaces and encased in a hydrated polymeric matrix. Bacteria in biofilms are more resistant to the immune system and to antibiotics than their free-living planktonic counterparts. Thus, biofilm-related infections are persistent and often show recurrent symptoms. The metal chelator EDTA is known to have activity against biofilms of gram-positive bacteria such as Staphylococcus aureus. EDTA can also kill planktonic cells of Proteobacteria like Pseudomonas aeruginosa. In this study we demonstrate that EDTA is a potent P. aeruginosa biofilm disrupter. In Tris buffer, EDTA treatment of P. aeruginosa biofilms results in 1,000-fold greater killing than treatment with the P. aeruginosa antibiotic gentamicin. Furthermore, a combination of EDTA and gentamicin results in complete killing of biofilm cells. P. aeruginosa biofilms can form structured mushroom-like entities when grown under flow on a glass surface. Time lapse confocal scanning laser microscopy shows that EDTA causes a dispersal of P. aeruginosa cells from biofilms and killing of biofilm cells within the mushroom-like structures. An examination of the influence of several divalent cations on the antibiofilm activity of EDTA indicates that magnesium, calcium, and iron protect P. aeruginosa biofilms against EDTA treatment. Our results are consistent with a mechanism whereby EDTA causes detachment and killing of biofilm cells.Biofilms consist of groups of bacteria attached to surfaces and encased in a hydrated polymeric matrix. Bacterial biofilms are abundant in the environment and are involved in several human bacterial infections (reviewed in references 11, 14, and 31). Of medical importance, biofilms can withstand host immune responses (19)(20)(21) and are much more resistant to antibiotic treatments than their nonattached, individual, free-living (planktonic) counterparts (28,36). For these reasons, biofilm infections are persistent, and individuals often show recurring symptoms following antibiotic therapy. One of the best-studied models for biofilm formation is the bacterium Pseudomonas aeruginosa (reviewed in references 27 and 30), which causes many types of infections, including biofilm-associated chronic lung infections in cystic fibrosis patients, acute ulcerative keratitis in users of extended-wear soft contact lenses, and bacteremia in severe-burn victims.The metal chelator EDTA has been shown to cause lysis, loss of viability, and increased sensitivity of planktonic Proteobacteria to a variety of antibacterial agents (reference 13; reviewed in references 25, 29, and 40). This has led to the use of EDTA as a preservative in many products. Little is known about the influence of EDTA on biofilms of Proteobacteria. Raad et al. (32,33) have shown that EDTA combined with minocycline is an effective treatment for microorganisms embedded in biofilms on catheter surfaces. Their studies focused on Staphylococcus epidermidis, Staphylococcus aureus, and Candida albicans; however, they also reported two cases of...

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“…The lag time started increasing at calcium chloride concentration of 4 g/l. This phenomenon can be attributed due to the microbial aggregates formed by calcium chloride at higher concentrations (Huang and Pinder, 1995). The microbial aggregates reduced the free microorganisms and thus the effective microbes accessing available substrate in the medium.…”

Section: Effect Of Salts On Bacterial Growthmentioning

confidence: 99%

Evaluation and elimination of inhibitory effects of salts and heavy metal ions on biodegradation of Congo red by Pseudomonas sp. mutant

Gopinath¹,

Kathiravan²,

Srinivasan

et al. 2011

Bioresource Technology

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Effects of calcium on development of anaerobic acidogenic biofilms

Cited by 54 publications

References 11 publications

Study on the Mass Transfer Enhancement in Biofilms Applied in Papermaking Wastewater Treatment

Study on the Mass Transfer Enhancement in Biofilms Applied in Papermaking Wastewater Treatment

Chelator-Induced Dispersal and Killing of Pseudomonas aeruginosa Cells in a Biofilm

Evaluation and elimination of inhibitory effects of salts and heavy metal ions on biodegradation of Congo red by Pseudomonas sp. mutant

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