Effects of Calcium Hydroxide and Tumor Growth Factor-β on Collagen Synthesis in Subcultures I and V of Osteoblasts

Abstract

Collagen protein synthesis by osteoblasts is influenced by transforming growth factor-beta (TGF-beta 1) and is essential to bone formation. The effectiveness of TGF-beta 1 depends on efficient delivery of the growth factor to target cells, adequate binding to cell surface receptors, and an optimum environment for promotion of collagen synthesis. The effects of calcium hydroxide (Ca(OH)2), TGF-beta 1, and Ca(OH)2/TGF-beta 1 co-administration on total protein, collagen protein, and noncollagen protein synthesis by early (subculture I) and late (subculture V) osteoblast cultures were tested. TGF-beta 1 significantly increased all protein synthesis in subculture I osteoblasts (p = 0.001; p < 0.001; p = 0.019). Ca(OH)2/TGF-beta 1 co-administration significantly increased total protein and collagen protein levels in subculture I osteoblasts as well (p = 0.048; p = 0.012). TGF-beta 1 increased total protein and collagen protein synthesis significantly in subculture V cells (p = 0.025; p = 0.01). These data indicate that co-administration of Ca(OH)2 and TGF-beta 1 enhances collagen synthesis by osteoblasts and may have implications for the clinical setting.

IGF‐I and TGF‐Beta 1 incorporated in a poly(D,L‐lactide) implant coating stimulates osteoblast differentiation and collagen‐1 production but reduces osteoblast proliferation in cell culture

“…Comparable results were seen by treating an osteoblastic cell line or porcine bone cells with IGF‐I 32, 33. The addition of TGF‐β1 to late osteoblast cell cultures increased the collagen production 34. The combination of growth factors like IGF‐I and TGF‐β1 had a synergistic effect on matrix apposition of fetal rat calvaria in vitro 35…”

IGF-I and TGF-beta 1 incorporated in a poly(d,l-lactide) implant coating maintain their activity over long-term storage—cell culture studies on primary human osteoblast-like cells

“…The cells showed a similar proliferation compared to the controls but a significantly enhanced production of procollagen 1 independently from the incubation time with the implant. Other studies were also able to demonstrate that the application of IGF-I or TGF-b1 into the culture medium enhanced the production of collagen in osteoblast culture [31][32][33][34][35]. These studies used freshly prepared growth factor solutions or the growth factor was incorporated into a membrane.…”

TGF‐β1 alone and in combination with calcium hydroxide is synergistic to TGF‐β1 production by osteoblasts in vitro

“…However, this could be explained by the inhibitory effects of TGF-β1 in all of the groups. As previously shown, the combination of Ca(OH) 2 and TGF-β1 is anabolic in cultures of osteoblasts ( Jaunberzins et al 2000).…”

“…Pfeilschifter et al (1988) found that TGF-β1 inhibits bone resorption. As previously shown, the combination of Ca(OH) 2 and TGF-β1 is anabolic in cultures of osteoblasts ( Jaunberzins et al 2000). This inhibition may be due to down-regulation of IL-6 production.…”