Effects of calcium channel blockers on hyaluronidase-induced capillary vascular permeability

Abstract

Inflammation and increased capillary permeability is a significant aspect of the pathogenesis of many diseases including atherosclerosis. L-type calcium channel blockers (CCB) are commonly used as cardiovascular drugs. Amlodipine, lacidipine, and nicardipine were evaluated for anti-inflammatory activity on the paw oedema produced by carrageenan. The effect of these drugs was compared with the activity of indomethacin. Their effects on vascular permeability were also tested by hyaluronidase-induced capillary permeability. In our animal experiments, amlodipine decreased the carrageenan-induced paw oedema at doses of 1, 3, and 6 mg kg(-1) by 27.3%, 43.7%, and 67.3% four hour after carrageenan administration; the same doses of lacidipine and nicardipine decreased paw oedema by 37.1%, 55.6%, 76.4%, 11.2%, 31.0%, 91%; and indomethacin decreased oedema by 38.2% at a dose of 6 mg kg(-1). Lacidipine significantly inhibited the hyaluronidase-induced increase in capillary permeability at doses of 1, 3, and 6 mg kg(-1) compared with the control group. However, amlodipine and nicardipine significantly inhibited the hyaluronidase-induced increase in capillary permeability at 3 and 6 mg kg(-1) doses. A 6 mg kg(-1) dose of indomethacin significantly decreased the capillary permeability which was increased by hyaluronidase. These results suggest that CCBs can be efficient anti-inflammatories, and can also significantly decrease capillary permeability.

Nicardipine vs. Saline Injection as Treatment for Peyronie’s Disease: A Prospective, Randomized, Single-Blind Trial

“…Halici et al. reported that nicardipine can be efficient anti‐inflammatories, and can also significantly decrease capillary permeability in the rats study [31]. Gomes et al.…”

“…Indeed, there are no reports of nicardipine for the treatment of PD. Halici et al reported that nicardipine can be efficient anti-inflammatories, and can also significantly decrease capillary permeability in the rats study [31]. Gomes et al reported that nicardipine improves experimental asthma by impairing inflammation, and could potentially be beneficial in the treatment of asthma [32].…”

The Effects of Amlodipine on the Biochemical and Histopathological Changes in the Rabbit Ileum Subjected to Ischemia-Reperfusion

“…In previous studies, we demonstrated that amlodipine exerts anti-inflammatory, anti-osteoporotic, and protective effects against ovarian ischemia-reperfusion (I/R) damage [12,13]. Amlodipine has also been clinically and experimentally shown to have antiatherogenic effects because it decreases and/or blocks inflammation from forming in the atheroma plaques on the vascular endothelium by inhibiting nitric oxide synthase activity [14].…”

Dynamic, size-selective effects of protamine sulfate and hyaluronidase on the rat glomerular filtration barrier in vivo

“…Investigations that have tried to modify the charge of the GC (15,54) or to reduce the impact of it via enzymatic ESL degradation have demonstrated increases in the glomerular permeability to albumin as a consequence of such pertubations (27,28,51). For example, hyaluronidase (hyase), which cleaves hyaluronan, chondroitin, and chondroitin sulfate, has been demonstrated to decrease the ESL and increase the permeability of peripheral and glomerular capillaries (16,21,25,27,28,36). In some of these studies, negatively charged albumin [radius 36 Å and sieving coefficient () ϳ 0.0001 (22)], which according to pore theory (the two-pore model) would permeate the GFB exclusively through larges pores (radius ϳ115-120 Å), has been compared with hyperpermeable neutral Ficoll (radius 36 Å) passing mainly through small pores (radius ϳ37.5 Å) and having a high (ϳ0.1) (2).…”