It has been shown in preceding papers how calcium-binding substances (Ca-b.s.) act on total absorption (SOGNEN 1961(SOGNEN & 1965, gastric emptying time and transit (SOGNEN 1965 d) in the intact animal, as well as on absorption from isolated and perfused intestinal loops in situ (SOGNEN 1965 c & e). Further, it has been shown how the renal excretion of sulphanilamide and urine flow behave in the presence of Ca-b.s. (SOGNEN 1965 b).In none of the experiments previously described has it been possible to study the transepithelial translocation alone without considering alterations in blood circulation and other factors that may vary in the intact animal or in situ.In our work the transport in vitro of glucose and a sulphonamide has therefore been investigated by WILSON & WISEMAN'S method (1 954), both in calcium-free Ringer solution and in the presence of Ca-b.s.
Material and MethodsMale rats weighing about 200 g were killed by a blow on the neck. The abdomen and the thorax were opened, but the diaphragm was left intact. The thoracic vessels were cut. Bleeding took place through the thoracic cavity and the nose.The intestine from the duodenojejunal flexure to the caecum was washed with a 0.9% sodium chloride solution. It was removed quickly from the mesentery in the direction from the caecum and forwards, transferred to a channel containing a 0.9 % sodium chloride solution and everted in this over a glass rod.Four segments were taken from the oral end of the intestinal loop. The specimens were about 5 cm long and prepared in rapid succession. They were incubated at Acla pharrnacologica, vol. 22, f a x . I .
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