Effects of calcium, alginate, and calcium-alginate immobilization on growth and tropane alkaloid levels of a stable suspension cell line of Datura innoxia Mill

Gontier, Éric; Sangwan, Brigitte; Barbotin, Jean‐Noël

doi:10.1007/bf00232951

Cited by 53 publications

(17 citation statements)

References 12 publications

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“…Lower concentrations also favoured the release of the secondary metabolite into the medium. These results were almost similar to those obtained by Gontier et al (1994) with suspension culture of Datura innoxia where addition of CaCl 2 at 10 mM increased the intracellular content of scopolamine and hyoscyamine approximately 10 times. Lowest concentration of CaCl 2 (i.e.…”

Section: Effect Of Abiotic Elicitors On Growth and Colchicine Contentsupporting

confidence: 93%

“…An added biotechnological benefit is that they also promote release of the metabolites into the medium (PittaAlvarez et al 2000a). Various biotic and abiotic elicitors have been used to increase natural product in plant cell cultures for biotechnological purposes (Gontier et al 1994, Yukimune et al 1996, Ketchum et al 1997, Szabo et al 1999, Chen and Chen 2000, Mukherjee et al 2000, Spollansky et al 2000, Pitta-Alvarez et al 2000b.…”

Section: Introductionmentioning

confidence: 98%

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Aluminium Chloride Enhances Colchicine Production in Root Cultures of Gloriosa superba

Ghosh

Ghosh²,

Jha

2006

Biotechnol Lett

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Root cultures of Gloriosa superba were treated with 5 mM: methyl jasmonate and 125 microM: AlCl3 which enhanced the intracellular colchicine content of the roots by 50-fold and 63-fold, respectively. Ten millimolar of CaCl2 and 1 mM: CdCl2 enhanced biomass significantly (7- to 8.6-fold, respectively) while maximum release of colchicine into the medium was obtained with 10 mM: CdCl2. Casein hydrolysate, yeast extract and silver nitrate had no significant effect on growth and colchicine accumulation in root cultures.

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Section: Effect Of Abiotic Elicitors On Growth and Colchicine Contentsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 98%

Aluminium Chloride Enhances Colchicine Production in Root Cultures of Gloriosa superba

Ghosh

Ghosh²,

Jha

2006

Biotechnol Lett

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“…Alginates are polysaccharides derived from seaweed and crosslinked by divalent cations (Draget et al, 2005). Alginates have various applications in industry, medicine and agriculture (Smidsrød and Skjåk-Braek, 1990;Gontier et al, 1994). In plant tissue culture, alginic acid has been used mostly as a replacement for agarose for cell immobilization in bioreactors (Payne et al,1988;Asada and Shuler, 1989;Draget et al, 2005) and for mass production of encapsulated plant material for various purposes such as cryopreservation or production of artificial seeds (Redenbaugh et al, 1991;Patel et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Cells entrapped in alginate are easily manipulated with less risk of physical damage (Draget et al, 1988;Niedz, 1993). The physiological responses of embedded cells are linked to the physical and chemical properties of alginates (Gontier et al, 1994). The preferred alginate for plant tissue culture is a linear copolymer of L-guluronic (G) and D-mannuronic (M) acid residues.…”

Section: Introductionmentioning

confidence: 99%

Embedding in Filter-Sterilized Alginate Enhances Brassica Oleracea L. Protoplast Culture

Kiełkowska¹,

Adamus²

2015

Acta Biologica Cracoviensia S. Botanica

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The influence of sodium alginate sterilization on the viability and mitotic activity of embedded protoplasts was studied in protoplasts of Brassica oleracea subsp. alba and rubra isolated from hypocotyl tissue and leaves of seedlings or plants grown in vitro. Both leaf and hypocotyl-derived protoplasts were more viable and divided more frequently when embedded in filtrated alginate. Division frequency was highest in cv. Reball F1 and the mitotic activity of its protoplasts was three times higher when embedded in filtrated alginate (36.1 ± 6.8%) than when cultured in autoclaved alginate (10.9 ± 5.0%). Protoplast-derived calli colonies were transferred to solid regeneration media and plants of all tested accessions were obtained.K Ke ey y w wo or rd ds s: : Brassica, calcium alginate layers, mitotic division, protoplast viability, regeneration.ACTA BIOLOGICA CRACOVIENSIA Series Botanica 56

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“…Cell immobilization techniques also stimulate the synthesis of secondary compounds and their secretion from the cell (Gilleta et al 2000). Another advantage of cell immobilization techniques is that cell harvest and the reuse of media are possible in that only the necessary product is purified from the media (Gontier et al 1994). Diverse immobilization matrixes such as polyacrylamide, calcium alginate, agarose, nylon, gelatin, polyphenyleneoxide, polyurethane foam, and glass fibers have been used to establish highly productive and easy-to-purify tissue culture systems (Smetanska 2008).…”

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confidence: 99%

Enhanced production and secretion of rutin and GABA in immobilized cells of mulberry tree (Morus bombycis K.)

Han

Lee

Song

et al. 2011

Plant Cell Tiss Organ Cult

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Cell immobilization has been proposed as a useful technique for mass production and efficient purification of secondary metabolites. In this study, we compared the bio-productivity of ligand-free and Ca-alginate-immobilized mulberry cells for rutin and c-amino butyric acid (GABA). In the leaves of Subong mulberry plants (M. bombycis K.) grown in a greenhouse, GABA accumulated as the leaves aged; a more than a 20-fold increase of GABA was observed in leaves undergoing senescence than in younger leaves. In contrast, more rutin was detected in mature leaves than in young leaves and those undergoing senescence. The production of total proteins in ligand-free leaf callus cells dramatically increased until 6 days after incubation in liquid suspension media (from 6.5 mg/g callus at day 0-14.5 mg/g callus), and by day 15 dropped to levels similar to those seen in the 0-day control. In contrast, immobilized cells showed a slight increase and then an insignificant decrease in protein content during the 15-day incubation period. Interestingly, immobilized mulberry cells more efficiently produced and secreted rutin and GABA into the suspension media than ligand-free cells. KN, a cytokinin, enhanced this production while 2,4-dichlorophenoxyacetic acid(2,4-D), an auxin, alleviated the effect of KN. As a result, incubation of the immobilized Subong cells in a full-strength Murashige and Skoog (MS) liquid medium containing 1 mg/l of 2,4-D and 0.1 mg/l KN, among the hormone combinations in the medium we tested, produced the highest amounts of rutin (8.2 lg/g callus cells) and GABA (305 lg/g callus cells) and secreted the largest amounts into the suspension media.

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Effects of calcium, alginate, and calcium-alginate immobilization on growth and tropane alkaloid levels of a stable suspension cell line of Datura innoxia Mill

Cited by 53 publications

References 12 publications

Aluminium Chloride Enhances Colchicine Production in Root Cultures of Gloriosa superba

Aluminium Chloride Enhances Colchicine Production in Root Cultures of Gloriosa superba

Embedding in Filter-Sterilized Alginate Enhances Brassica Oleracea L. Protoplast Culture

Enhanced production and secretion of rutin and GABA in immobilized cells of mulberry tree (Morus bombycis K.)

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