Effects of caffeine on bone cells and bone development in fast-growing rats

Wink, Carole S.; Rossowska, M; Nakamoto, Tetsuo

doi:10.1002/(sici)1097-0185(199609)246:1<30::aid-ar4>3.0.co;2-j

Cited by 31 publications

(26 citation statements)

References 22 publications

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“…Moreover, a study in fast-growing rats showed that rat pups from lactating dams received a 20% protein diet with caffeine experience disruption of osteoblasts and retarded bone development. 7 This finding infers that caffeine intake in early life exerts effects on osteoblasts and bone development. 7 Conversely, our results showed that caffeine supplementation (0.2% in diet) in weaned rats for 20 weeks significantly enhanced osteoclast differentiation, but did not affect osteoblast differentiation from bone marrow cells.…”

Section: Discussionmentioning

confidence: 79%

“…7 This finding infers that caffeine intake in early life exerts effects on osteoblasts and bone development. 7 Conversely, our results showed that caffeine supplementation (0.2% in diet) in weaned rats for 20 weeks significantly enhanced osteoclast differentiation, but did not affect osteoblast differentiation from bone marrow cells. The significance of these different effects on osteoblasts in rats exposed to caffeine before and after weaning needs to be clarified.…”

Section: Discussionmentioning

confidence: 79%

“…5 However, Rapuri et al 6 indicated that intakes of caffeine in amounts >300 mg/day (approximately 514 g, or 18 oz, brewed coffee) accelerate bone loss at the spine in elderly postmenopausal women. Wink et al 7 also showed that if young, rapidly growing rats are exposed to caffeine, disruption of osteoblasts and retarded bone development occur. An in vitro study showed that caffeine may enhance the rate of osteoblast apoptosis and has potential deleterious effect on the osteoblast viability.…”

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confidence: 99%

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Caffeine enhances osteoclast differentiation from bone marrow hematopoietic cells and reduces bone mineral density in growing rats

Liu

Chen

Yang

et al. 2011

Journal Orthopaedic Research

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Caffeine-containing beverage consumption has been associated with low bone mass and increased fracture risk in some, but not most, observational studies. The effects of caffeine on bone metabolism are still controversial. We investigated the effects of caffeine on the differentiation of bone progenitor cells and bone mineral density (BMD) by in vitro and in vivo experiments. Low-concentration caffeine (0.005-0.1 mM) did not affect the bone marrow cell viability and alkaline phosphatase activity during osteoblast differentiation from bone marrow stromal cells, but it effectively enhanced the osteoclastogenesis from bone marrow hematopoietic cells and the bone resorption activity by pit formation assay. Moreover, caffeine effectively enhanced the receptor activator of NF-kB ligand (RANKL), but reduced the osteoprotegerin protein expressions in osteoblast MC3T3-E1 cells. Caffeine could also increase the cyclooxygenase-2 (COX-2) protein expression and prostaglandin (PG)E 2 production in cultured neonatal mouse calvariae. In animal study, BMD in lumbar vertebra, femur, or tibia was significantly lowered in growing rats supplemented with 0.2% caffeine in diets for 20 weeks compared with the control group. The calcium contents in tibia and femur of caffeine-treated rats were also lower than that in the control group. The osteoclastogenesis of bone marrow cells isolated from caffeine-treated rats was markedly enhanced as compared with the control group. Taken together, these results suggest that caffeine may reduce BMD in growing rats through the enhancement in osteoclastogenesis. Caffeine may possess the ability to enhance a COX-2/PGE 2 -regulated RANKL-mediated osteoclastogenesis. ß

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 79%

mentioning

confidence: 99%

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Caffeine enhances osteoclast differentiation from bone marrow hematopoietic cells and reduces bone mineral density in growing rats

Liu

Chen

Yang

et al. 2011

Journal Orthopaedic Research

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“…The substance was administered in a dose of 30 mg/day, which according to the literature should disturb the rat's prenatal development [22,24,[27][28][29][30].…”

Section: Methodsmentioning

confidence: 99%

“…In the sternum area, administering caffeine caused agenesis of its parts, and in the vertebra, fusion of vertebral bodies, especially in the lumbar and sacral regions. Other researchers [17,[25][26][27] have analyzed the morphology of developing bones using visual data (obtained with a scanning electron microscope and a transmission electron microscope), and mineral content on the basis of spectrophotometric analysis.…”

Section: Introductionmentioning

confidence: 99%

Use of X-ray microprobe to diagnose bone tissue demineralization after caffeine administration

Tomaszewski¹,

Olchowik²,

Tomaszewska³

et al. 2012

Folia Histochem Cytobiol.

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Abstract:Caffeine is a methylxanthine which permeates the placenta. In studies on animals, it has been shown to produce teratogenic and embryotoxic effects in large doses. The objective of this study was to assess the influence of caffeine on the development of bone tissue, with particular reference to elemental bone composition using an X-ray microprobe. The research was conducted on rats. The fertilized females were randomly divided into an experimental and a control group. The experimental group was given caffeine orally in 30 mg/day doses from the 8 th to the 21 st day of pregnancy, while the control group was given water. The fetuses were used to assess the growth and mineralization of the skeleton. On the basis of double dyeing, a qualitative analysis of the bone morphology and mineralization was conducted. For calcium and potassium analysis, an X-ray microprobe was used. In 67 fetuses from the experimental group, changes in skeleton staining with the alcian-alizarin method were noticed. The frequency of the development of variants in the experimental group was statistically higher. In the experimental group, a significant decrease in the calcium level, as well as an increase in the potassium level, was observed. The X-ray microprobe's undoubted advantage is that is offers a quick qualitative and quantitative analysis of the elemental composition of the examined samples. Employing this new technique may furnish us with new capabilities when investigating the essence of the pathology process.

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Favorable effect of moderate dose caffeine on the skeletal system in ovariectomized rats

Folwarczna

Pytlik

Zych

et al. 2013

Molecular Nutrition Food Res

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Effects of caffeine on bone cells and bone development in fast-growing rats

Cited by 31 publications

References 22 publications

Caffeine enhances osteoclast differentiation from bone marrow hematopoietic cells and reduces bone mineral density in growing rats

Caffeine enhances osteoclast differentiation from bone marrow hematopoietic cells and reduces bone mineral density in growing rats

Use of X-ray microprobe to diagnose bone tissue demineralization after caffeine administration

Favorable effect of moderate dose caffeine on the skeletal system in ovariectomized rats

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