Effects of caffeine on behavioral and inflammatory changes elicited by copper in zebrafish larvae: Role of adenosine receptors

Cruz, Fernanda Fernandes; Leite, Carlos Eduardo; Kist, Luiza Wilges; Oliveira, Giovanna Medeiros de; Bogo, Maurı́cio Reis; Bonan, Carla Denise; Morrone, Fernanda Bueno

doi:10.1016/j.cbpc.2017.01.007

Cited by 18 publications

(14 citation statements)

References 49 publications

(56 reference statements)

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“…A previous study demonstrated that low concentrations of copper (<10 µM) did not induce mortality [16]. At the concentration of 10 µM, although it did not cause a significant decrease of zebrafish larvae survival, copper sulfate was able to induce oxidative stress and a marked increase of inflammatory markers [17]. A concentration of 25 µM induced a 75-80% death rate [18].…”

Section: Protective Activity Of the Ethanol Extract Of C Cyrtophyllumentioning

confidence: 95%

Anti–Inflammatory and Antioxidant Properties of the Ethanol Extract of Clerodendrum Cyrtophyllum Turcz in Copper Sulfate-Induced Inflammation in Zebrafish

Nguyen

Kim

et al. 2020

Antioxidants

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Oxidative stress and inflammation are commonly present in many chronic diseases. These responses are closely related to pathophysiological processes. The inflammatory process can induce oxidative stress and vice versa through the activation of multiple pathways. Therefore, agents with antioxidant and/or anti-inflammatory activities are very useful in the treatment of many pathologies. Clerodendrum cyrthophyllum Turcz, a plant belonging to the Verbenaceae family, is used in Vietnamese traditional medicine for treating migraine, hypertension, inflammation of the throat, and rheumatic arthritis. Despite its usefulness, studies on its biological properties are still scarce. In this study, ethanol extract (EE) of leaves of C. cyrtophyllum showed protective activity against CuSO4 toxicity. The protective activity was proven to relate to antioxidant and anti-inflammatory properties. EE exhibited relatively high antioxidant activity (IC50 of 16.45 µg/mL) as measured by DPPH assay. In an in vivo anti-antioxidant test, three days post fertilization (dpf) zebrafish larvae were treated with different concentrations of EE for 1 h and then exposed to 10 µM CuSO4 for 20 min to induce oxidative stress. Fluorescent probes were used to detect and quantify oxidative stress by measuring the fluorescent intensity (FI) in larvae. FI significantly decreased in the presence of EE at 5 and 20 µg/mL, demonstrating EE’s profound antioxidant effects, reducing or preventing oxidative stress from CuSO4. Moreover, the co-administration of EE also protected zebrafish larvae against oxidative damage from CuSO4 through down-regulation of hsp70 and gadd45bb expression and upregulation of sod. Due to copper accumulation in zebrafish tissues, the damage and oxidative stress were exacerbated overtime, resulting in the upregulation of genes related to inflammatory processes such as cox-2, pla2, c3a, mpo, and pro- and anti-inflammatory cytokines (il-1ß, il-8, tnf-α, and il-10, respectively). However, the association of CuSO4 with EE significantly decreased the expression of cox-2, pla2, c3a, mpo, il-8, and il-1ß. Taken together, the results suggest that EE has potent antioxidant and anti-inflammatory activities and may be useful in the treatment of various inflammatory diseases.

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Section: Protective Activity Of the Ethanol Extract Of C Cyrtophyllumentioning

confidence: 95%

Anti–Inflammatory and Antioxidant Properties of the Ethanol Extract of Clerodendrum Cyrtophyllum Turcz in Copper Sulfate-Induced Inflammation in Zebrafish

Nguyen

Kim

et al. 2020

Antioxidants

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“…This lesion is probably related to the production of reactive oxygen species (ROS), generated through the copper reduction process, which is also responsible for promoting an inflammatory process (Brown and Borutaite, 2001;Oyinloye et al, 2015). Recently, some studies have shown the involvement of the purinergic system in this model of inflammation (Cruz et al, 2017;Leite et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

P2X7R and PANX-1 channel relevance in a zebrafish larvae copper-induced inflammation model

Cruz

Menezes

Kist

et al. 2019

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…The effect of caffeine on physiology and behaviour of animals is widely studied in invertebrates as well as vertebrates (Capiotti et al, 2011;Chen et al, 2008;Cruz et al, 2017;Mustard, 2014;Wu et al, 2009). In Apis mellifera caffeine modulates learning and memory; it specifically affects performance during acquisition but not the processes involved in the formation of long term memory (Mustard et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…This latter effect was due to the misalignment of muscle fibres and motor neurone defects in treated embryos. Zebra fish embryos also showed reduced mobility when exposed to caffeine possibly as a result of decreased expression of adenosine receptors (Cruz et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Caffeine administration alters the behaviour and development of Galleria mellonella larvae

Maguire

Kunc

Hyršl

et al. 2017

Neurotoxicology and Teratology

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The effect of feeding caffeine on the behaviour and neural proteome of Galleria mellonella larvae was assessed. Caffeine was administered to larvae by force feeding and the metabolites theobromine and theophylline were subsequently detected by RP-HPLC analysis. Administration of caffeine to larvae resulted in reduced movement and a reduction in the formation of pupae. The production of the muscle relaxant theophylline may contribute to the reduction in larval movement. Analysis of the changes in proteome of the brain and surrounding tissues of caffeine fed larvae revealed an increase in the abundance of immune related proteins such as immune-related Hdd1 (6.28 fold increase) and hemolin (1.68 fold increase), ATPase associated proteins such as H+ transporting ATP synthase O subunit isoform 1 (1.87 fold increase) and H+ transporting ATP synthase delta subunit (1.53 fold increase) and proteins indicative of brain trauma such as troponin T transcript variant B, partial (1.55 fold increase). Proteins involved in development and protein degradation such as SUMO-activating enzyme subunit 1 (3.08 fold decrease) and chitin deacetylase, partial (3.67 fold decrease) were decreased in abundance. The results presented here indicate that caffeine is metabolised in a similar way in G. mellonella larvae to that in mammals and results in a variety of behavioural and developmental alterations. Utilisation of insects for studying the effects of caffeine and other neuroactive compounds may offer new insights into their mode of action and reduce the need to use mammals for this type of analysis.

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Effects of caffeine on behavioral and inflammatory changes elicited by copper in zebrafish larvae: Role of adenosine receptors

Cited by 18 publications

References 49 publications

Anti–Inflammatory and Antioxidant Properties of the Ethanol Extract of Clerodendrum Cyrtophyllum Turcz in Copper Sulfate-Induced Inflammation in Zebrafish

Anti–Inflammatory and Antioxidant Properties of the Ethanol Extract of Clerodendrum Cyrtophyllum Turcz in Copper Sulfate-Induced Inflammation in Zebrafish

P2X7R and PANX-1 channel relevance in a zebrafish larvae copper-induced inflammation model

Caffeine administration alters the behaviour and development of Galleria mellonella larvae

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