Effects of caffeic acid phenethyl ester application on dentin MMP-2, stability of bond strength and failure mode of total-etch and self-etch adhesive systems

Pedrosa, Vivianne Oliveira; França, Fabiana Mantovani Gomes; Turssi, Cecília Pedroso; Amaral, Flávia Lucisano Botelho do; Teixeira, Lucas Novaes; Martinez, Elizabeth Ferreira; Basting, Roberta Tarkany

doi:10.1016/j.archoralbio.2018.06.012

Cited by 7 publications

(10 citation statements)

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“…CAPE was procured in pro-analysis (PA) form and solubilized in ethanol following the manufacturer's instructions. Specifically, 10 mg of CAPE was diluted in 35.17 mL of ethanol under stirring for 60 seconds, resulting in a stock solution with a concentration of 9.29% CAPE 19,20 . This "stock" solution was then fractionated and further diluted in ethanol to achieve concentrations of 0.05%, 0.1%, 0.5%, and 2.5%.…”

Section: Preparation Of Collagen Matricesmentioning

confidence: 99%

“…Among the polyphenolic compounds, caffeic acid phenethyl ester (CAPE) is a biologically active ingredient found in bee propolis, exhibiting antioxidant and anti-inflammatory actions 16 . It is an outstanding inhibitor of MMP-2 and MMP-9 17,18 in other tissues, such as in the accelerated healing of alveolar bone defects 19 , and has exhibited anti-enzyme activity in dentin 20,21 . Additionally, CAPE has shown anti-enzyme activity in dentin.…”

Section: Introductionmentioning

confidence: 99%

“…Studies have suggested that the mechanism of action of CAPE is related to the inactivation of the proenzyme, a precursor of proteolysis of MMPs, in addition to stimulating the activity of tissue inhibitors of metalloproteinases (TIMPs) 6 . Due to the inhibitory effect on MMPs, studies have suggested that its use may slow down the degradation of the hybrid layer 14,19,20 , but the optimal concentration of CAPE for this effect has not yet been established. When solubilized in dimethyl sulfoxide (DMSO), 5% CAPE reduced nanoleakage in the hybrid layer 14 and also significantly reduced dentin MMP-2 concentration 19 and gelatinolytic activity 20 when using a conventional three-step adhesive, especially when applied prior to acid etching at a 0.1% concentration.…”

Section: Introductionmentioning

confidence: 99%

“…Due to the inhibitory effect on MMPs, studies have suggested that its use may slow down the degradation of the hybrid layer 14,19,20 , but the optimal concentration of CAPE for this effect has not yet been established. When solubilized in dimethyl sulfoxide (DMSO), 5% CAPE reduced nanoleakage in the hybrid layer 14 and also significantly reduced dentin MMP-2 concentration 19 and gelatinolytic activity 20 when using a conventional three-step adhesive, especially when applied prior to acid etching at a 0.1% concentration. However, considering that DMSO is not only a synthetic inhibitor of MMPs 22 but also an apoptotic and polar solvent, its volatilization from the dentin substrate can hinder the process of volatilizing solvents (water and ethanol) present in adhesive systems 22 .…”

Section: Introductionmentioning

confidence: 99%

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Stability of dentin matrix treated with caffeic acid phenethyl ester at different concentrations

Damázio,

Basting,

Bridi

et al. 2024

Braz. J. Oral Sci.

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Aim: The aim of this study was to investigate the impact of pretreatment with ethanolic solutions of caffeic acid phenethyl ester (CAPE) at varying concentrations on the dentin collagen matrix, specifically focusing on its biomodification potential. This was assessed through evaluations of the modulus of elasticity and changes in mass. Methods: Seventy dentin collagen matrices (demineralized sticks) were prepared to receive treatments with ethanolic solutions of CAPE at concentrations of 0.05%, 0.1%, 0.5%, or 2.5%, or with control treatment solutions (distilled water or ethanol) for one hour. The dentin matrices were evaluated for modulus of elasticity and mass before (baseline), immediately after treatment (immediately), and after storage in Simulated Body Fluid (SBF) for time intervals of 1 and 3 months. Results: Generalized linear models for repeated measures over time indicated no significant differences between groups (p=0.7530) or between different time points (p=0.4780) in terms of the modulus of elasticity. Regarding mass variation, no differences were observed in the time interval between 1 month and the immediate time (p=0.0935). However, at the 3-month mark compared to the immediate time, the 0.1% CAPE group exhibited less mass loss compared to the water group (p=0.0134). Conclusion: This study concludes that various concentrations of CAPE in an ethanolic solution did not affect the modulus of elasticity of dentin, suggesting that CAPE lacks biomodifying potential in this context. However, it was observed that 0.1% CAPE positively influenced the variation in mass over different evaluation time intervals.

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Section: Preparation Of Collagen Matricesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Stability of dentin matrix treated with caffeic acid phenethyl ester at different concentrations

Damázio,

Basting,

Bridi

et al. 2024

Braz. J. Oral Sci.

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“…13 Caffeic acid phenethyl ester (CAPE) is an active derivative of propolis, which is a potent antioxidant, and its mechanism is based Iİ˙ on the inhibition of metalloproteinases. 15 A study on dentin has shown that CAPE can be used as an antioxidant before the restorative procedure. 16 It also possesses several biological activities such as antioxidant, antiviral, anti-inflammatory, and carcinostatic activities.…”

Section: Introductionmentioning

confidence: 99%

Does Caffeic Acid Phenethyl Ester as an Irrigation Solution Increase the Adhesive Quality of Root Canal Sealer?

Tosun

Karataşlıoğlu

2020

Journal of Advanced Oral Research

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Aim: To evaluate the effect of the caffeic acid phenethyl ester (CAPE) as a root canal irrigation agent on the push-out bond strength of the AH-Plus sealer. Materials and Methods: A total of 75 single-rooted teeth were decoronated and were randomly divided into 5 groups of 15 roots for irrigation protocols: Group NaOCl: 5.25 percent NaOCl; Group CAPE: 0.5 percent CAPE; Group NaOCl + ethylenediaminetetraacetic acid (EDTA): 5.25 percent NaOCl-17 percent EDTA; Group NaOCl + CAPE: 5.25 percent NaOCl-0.5 percent CAPE; and Group CAPE + EDTA: 0.5 percent CAPE-17 percent EDTA (for 3 min each group). All root canals were then obturated and 1-mm-thick horizontal slices were obtained from different root thirds of the root canal (coronal, middle, and apical, respectively). The groups were challenged with push-out tests. Modes of failure were determined under a stereomicroscope. Results: The CAPE-EDTA-treated group presented the highest mean bond strength in the coronal region of root dentin ( P < .05). The CAPE-treated group had a higher mean bond strength than the NaOCl-treated group ( P < .05). The mixed mode of failure was most predominant in all groups. Conclusion: Under the presented in vitro conditions, CAPE alone or in combination with EDTA or NaOCl demonstrated a positive effect that increased the push-out bond strength of the AH-Plus sealer to root dentin.

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Impact of Dentine Pretreatment with Matrix Metalloproteinase Inhibitors on Bond Strength of Coronal Composite Restorations: A Systematic Review and Meta-analysis of In Vitro Studies

et al. 2022

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Objectives Matrix metalloproteinase (MMP) enzymes participate in collagen matrix degradation, including in dentine, potentially compromising bond strength. Therefore, MMP inhibitors have been hypothesized to improve restoration bond strength and stability. This systematic review aimed to evaluate the influence of different MMP inhibitors applied as dentine surface pretreatments on the immediate (24 hours) and longer term (months) bond strength of direct coronal composite restorations. Materials and Methods This systematic literature review followed the Preferred Reporting Items for Systematic Review and Meta-analyses (PRISMA) statement. A systematic literature search of three databases (Ovid MEDLINE, Ovid Embase, and Google Scholar) was conducted independently by two reviewers from inception to April 2022. An adapted quality assessment tool was independently applied by two reviewers for risk of bias assessment. Statistical Analysis RevMan v5.4 software was used for meta-analyses. A random-effects model was used to generate mean differences with 95% confidence intervals for treatment and control comparisons. The Q-test and I2 -test were used to test for heterogeneity. The proportion of total variance across studies attributable to heterogeneity rather than chance was calculated. Overall effects were tested using the Z-test, while subgroup differences were tested using Chi-squared tests. Results Of 934 studies, 64 studies were included in the systematic review and 42 in the meta-analysis. Thirty-one MMP inhibitors were reported, three of which were included in the meta-analysis: 2% chlorhexidine (CHX), 0.3 M carbodiimide (EDC), and 0.1% riboflavin (RIBO). Pretreatment with 2% CHX for 30 and 60 seconds did not significantly improve bond strength compared with controls either immediately or after long-term ageing. However, pretreatment with 0.3 M EDC and 0.1% RIBO (but not CHX) significantly improved bond strength compared with control groups both immediately and over time. Most studies showed a medium risk of bias. Conclusions These in vitro findings pave the way for rationale clinical trialing of dentine surface pretreatment with MMP inhibitors to improve clinical outcomes.

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Effects of caffeic acid phenethyl ester application on dentin MMP-2, stability of bond strength and failure mode of total-etch and self-etch adhesive systems

Cited by 7 publications

References 50 publications

Stability of dentin matrix treated with caffeic acid phenethyl ester at different concentrations

Stability of dentin matrix treated with caffeic acid phenethyl ester at different concentrations

Does Caffeic Acid Phenethyl Ester as an Irrigation Solution Increase the Adhesive Quality of Root Canal Sealer?

Impact of Dentine Pretreatment with Matrix Metalloproteinase Inhibitors on Bond Strength of Coronal Composite Restorations: A Systematic Review and Meta-analysis of In Vitro Studies

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