Effects of cadmium on oxidative stress and cell apoptosis in Drosophila melanogaster larvae

Yang, Pingping; Yang, Xingran; Sun, Liran; Han, Xiaobing; Xu, Lu; Gu, Wei; Zhang, Min

doi:10.1038/s41598-022-08758-0

Cited by 22 publications

(15 citation statements)

References 41 publications

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“…It can also lead to changes in the collagen matrix, on which bone mineral is deposited, by stimulating osteoclast proliferation and activity. The latest studies indicate that the toxicity of cadmium may also result from its ability to induce oxidative stress [ 81 , 82 ]. Because this element is easily absorbed, it generally accumulates in bones in amounts that are dependent on the natural environment and diet [ 83 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Nano- and Microzinc Supplementation on the Mineral Composition of Bones of Rats with Induced Mammary Gland Cancer

Skrajnowska

Idkowiak

Szterk³

et al. 2023

Foods

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Background: The aim of this study was to determine changes in the mineral composition of the bones of rats with chemically induced mammary gland cancer and to attempt to establish whether a specific diet modification involving the inclusion of zinc ions in two forms—nano and micro—will affect the mineral composition of the bones. Methods: Female Sprague–Dawley rats were used for the research. The animals were randomly assigned to three experimental groups. All animals were fed a standard diet (Labofeed H), and selected groups additionally received zinc nanoparticles or microparticles in the amount of 4.6 mg/mL. To induce mammary cancer, the animals were given 7,12-dimethyl-1,2-benz[a]anthracene. The content of Ag, As, B, Ba, Cd, Cr, Cu, Mn, Ni, Pb, Rb, Se, Sr, Tl, U, and V was determined using ICP-MS, while that of Ca, Fe, K, Mg, Na, and Zn was determined using FAAS. Results: The use of a diet enriched with zinc nano- or microparticles significantly influenced the content of the elements tested. In the bones of rats fed a diet with zinc nanoparticles, changes were found in the content of Ca, Mg, Zn, Cd, U, V, and Tl, while in the case of the diet supplemented with zinc microparticles, there were differences in six elements—Ca, Mg, B, Cd, Ag, and Pb—compared to animals receiving an unsupplemented diet. Conclusions: The content of elements in the bone tissue of rats in the experimental model indicates disturbances of mineral metabolism in the tissue at an early stage of mammary cancer.

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Section: Discussionmentioning

confidence: 99%

Effect of Nano- and Microzinc Supplementation on the Mineral Composition of Bones of Rats with Induced Mammary Gland Cancer

Skrajnowska

Idkowiak

Szterk³

et al. 2023

Foods

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“…Exposure to heavy metals have been shown to induce oxidative stress in D. melanogaster, resulting in increased levels of malondialdehyde (MDA) as a marker of lipid peroxidation (Liu et al, 2020; Yang et al ., 2022). The level of MDA was significantly higher when compared to the control but significantly lower when compared with the resveratrol treated groups (p < 0.05).…”

Section: Discussionmentioning

confidence: 99%

CO-ADMINISTRATION OF RESVERATROL RESCUED LEAD-INDUCED TOXICITY INDROSOPHILA MELANOGASTER

Abdulazeez

Salisu²,

Onyawole

et al. 2023

Preprint

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Lead toxicity is a major environmental issue and has been associated with various health problems such as cognitive and behavioral impairments, reproductive defects and cellular oxidative stress. The study investigated the potential of resveratrol in mitigating the effects of lead-induced toxicity in Drosophila melanogaster. Three (3) days old Harwich strain adult D. melanogaster were orally exposed to Pb (60 mg/L), Succimer (10 mg/kg), and Resveratrol (50, 100 and 150 mg/kg). Biological and biochemical parameters (ALP, AST, TB, CB, and electrolytes levels), kidney function test (Ur and Cr), oxidative stress marker (MDA), and antioxidants (SOD, and CAT) were evaluated. Resveratrol significantly increased egg-laying, eclosion rate, filial generation output, locomotor activity, and life span of D. melanogaster in a dose-dependent manner up to 150 mg/kg of diet. Most biochemical parameters investigated were significantly rescued in the lead-poisoned fruit flies (p < 0.05) when co-treated with resveratrol. The results suggest that resveratrol has a protective effect against lead toxicity in Drosophila melanogaster and could potentially be used as a therapeutic agent for lead poisoning in humans.

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“…Cigarette smoke exposure was conducted in this study and evaluated through two tests: a trypan blue staining assay designed to detect cell damage in midgut larvae exposed to cigarette smoke (cells become discolored after being irritated by the smoke) and a crawling assay to compare the movement patterns of larvae in the control and exposure groups. Levels of trypan blue staining signal in the midgut served as an indicator of the extent of cell injury [ 24 ]. Larvae exposed to the trypan blue staining test exhibited cell death or successful exposure to cigarette smoke.…”

Section: Discussionmentioning

confidence: 99%

“…Observation of color changes in the larvae’s midgut (middle intestine) served as a reliable indicator of cellular injury. Dead tissue or cells were stained with trypan blue [ 23 ], and a darker midgut color in D. melanogaster larvae was associated with higher levels of cellular damage [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

Phenotypical and molecular assessments on the pharmacological effects of curcumin in Drosophila melanogaster

Rumata¹,

Purwaningsih²,

Asbah³

et al. 2023

Narra J

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Curcumin, a dietary polyphenol derived from turmeric's rhizome, exhibits a range of pharmacological activities, such as antioxidant, analgesic, antipyretic, and anti-inflammatory effects. It has been investigated for its therapeutic potential in chronic obstructive pulmonary disease (COPD) due to its ability to inhibit nuclear factor kappa B (NF-κB), modulate oxidative stress, impact cell viability, and regulate gene expression. However, most studies have been limited to in vitro conditions. To address this gap, we conducted in vivo experiments using the fruit fly Drosophila melanogaster to explore the antioxidant activities and biological significance of curcumin. Several parameters were assessed using different assays, including curcumin toxicity assay, fly survival, locomotor response to curcumin treatment (with or without cigarette smoke), trypan blue staining, larval crawling assays, and gene expression analysis. Our data revealed no significant differences in toxicity and locomotor tests across various curcumin concentrations. D. melanogaster tolerated curcumin at concentrations of 0.5 µM, 5 µM, 50 µM, and 500 µM, suggesting its safety without negatively impacting locomotor. Furthermore, curcumin at 5 µM extended the lifespan of D. melanogasterexposed to cigarette smoke, while reversing the negative effects of smoke exposure on gut cell viability and larval locomotor activity. In conclusion, curcumin administration appeared safe for D. melanogaster, with potential benefits for longevity and locomotory function. These findings support the idea that curcumin possesses in vivo antioxidant properties and may serve as a promising pharmacological agent. However, further study is needed to explore its potential applications in human health and disease management, particularly in the context of COPD.

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Effects of cadmium on oxidative stress and cell apoptosis in Drosophila melanogaster larvae

Cited by 22 publications

References 41 publications

Effect of Nano- and Microzinc Supplementation on the Mineral Composition of Bones of Rats with Induced Mammary Gland Cancer

Effect of Nano- and Microzinc Supplementation on the Mineral Composition of Bones of Rats with Induced Mammary Gland Cancer

CO-ADMINISTRATION OF RESVERATROL RESCUED LEAD-INDUCED TOXICITY INDROSOPHILA MELANOGASTER

Phenotypical and molecular assessments on the pharmacological effects of curcumin in Drosophila melanogaster

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