Effects of cadmium on intracellular cation homoeostasis in the yeast<i>Saccharomyces cerevisiae</i>

Kuang, Xin; Fang, Zhijia; Wang, Song; Shi, Ping; Huang, Zhiwei

doi:10.1080/02772248.2015.1074689

Cited by 14 publications

(9 citation statements)

References 31 publications

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“…Other studies suggest that the cytotoxicity of cadmium can be attributable to the interference of cadmium with intracellular cation homoeostasis. 39 40 Cadmium can upregulate the internal concentration of calcium in neurons, thereby affecting the synthesis and release of neurotransmitters, eventually leading to neuronal dysfunction. 40 …”

Section: Discussionmentioning

confidence: 99%

Associations between blood cadmium levels and cognitive function in a cross-sectional study of US adults aged 60 years or older

Wang

et al. 2018

BMJ Open

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ObjectivesThe relationship between cadmium exposure and cognition has been well studied in children. However, the association between environmental cadmium exposure and cognitive function has not been researched extensively in older adults. Our goal was to evaluate the association between cognitive function and blood cadmium levels in US adults aged 60 years or older.DesignA cross-sectional study.SettingThe US National Health and Nutrition Examination Survey (NHANES).ParticipantsA total of 2068 adults aged 60 years or older who completed four cognitive assessment tests and blood cadmium detection in two waves of NHANES (2011–2014).Main outcome measuresCognitive assessment was conducted by household interview or at a Mobile Examination Center (MEC) using the Consortium to Establish a Registry for Alzheimer’s Disease (CERAD) Word List Learning Test, the CERAD Word List Recall Test, the Animal Fluency Test and the Digit Symbol Substitution Test (DSST). We created a composite cognitive z-score to represent global cognitive function.ResultsThe median blood cadmium concentration in the study participants was 0.35 µg/L, and the IQR was 0.24–0.56 µg/L. In linear regression analyses, adjusting for demographics, behaviour and medical history, blood cadmium as a continuous variable was inversely associated with the composite z-score (μg/L, β=−0.11, 95% CI −0.20 to −0.03). Similarly, there was a significant association between quartiles of blood cadmium and composite z-score, with somewhat lower scores in the upper quartile of exposure (blood cadmium ≥0.63 µg/L) compared with those in the lower quartile of exposure (blood cadmium <0.25 µg/L) (μg/L, β=−0.14, 95% CI −0.25 to –0.03), and there was a trend by quartiles of blood cadmium (P<0.0001).ConclusionsOur findings suggest that increased blood cadmium is associated with worse cognitive function in adults aged 60 years or older in the USA.

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Section: Discussionmentioning

confidence: 99%

Associations between blood cadmium levels and cognitive function in a cross-sectional study of US adults aged 60 years or older

Wang

et al. 2018

BMJ Open

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“…In a previous study, we observed that yeast was sensitive to cadmium; 150 M was the approximate lethal dose (ALD) of cadmium for BY4741 cells, and cadmium exhibited strong inhibitory effects on the growth of yeast (10). In this study, 300 M cadmium (2 times the ALD) was used to treat yeast harboring the essential gene overexpression library, in order to screen for anti-cadmium-stress genes.…”

Section: Resultsmentioning

confidence: 99%

“…Several previous investigations reported that cadmium can exert toxic effects through inhibition of ATPase activity (28), interference with cation uptake (10), and induction of cellular apoptosis (29)(30)(31). Many cation uptake and transport genes were found to play important roles in cadmium resistance in plants or microorganisms (9).…”

Section: Discussionmentioning

confidence: 99%

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Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae

Fang

Chen

Wang

et al. 2017

Appl Environ Microbiol

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The heavy metal cadmium is widely used and released into the environment, posing a severe threat to crops and humans. Saccharomyces cerevisiae is one of the most commonly used organisms in the investigation of environmental metal toxicity. We investigated cadmium stress and the adaptive mechanisms of yeast by screening a genome-wide essential gene overexpression library. A candidate gene, OLE1, encodes a delta-9 desaturase and was associated with high anti-cadmiumstress activity. The results demonstrated that the expression of OLE1 was positively correlated with cadmium stress tolerance and induction was independent of Mga2p and Spt23p (important regulatory factors for OLE1). Moreover, in response to cadmium stress, cellular levels of monounsaturated fatty acids were increased. The addition of exogenous unsaturated fatty acids simulated overexpression of OLE1, leading to cadmium resistance. Such regulation of OLE1 in the synthesis of unsaturated fatty acids may serve as a positive feedback mechanism to help cells counter the lipid peroxidation and cytoplasmic membrane damage caused by cadmium. IMPORTANCE A S. cerevisiae gene encoding a delta-9 desaturase, OLE1, was associated with high anti-cadmium-stress activity. The data suggest that the regulation of OLE1 in the synthesis of unsaturated fatty acids may serve as a positive feedback mechanism to help yeast cells counter the lipid peroxidation and cytoplasmic membrane damage caused by cadmium. The discovery of OLE1 involvement in membrane stability may indicate a novel defense strategy against cadmium stress.

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“…Then, the exponential growing cells (OD 600 = 0.5) were exposed to 65 μM CdCl 2 at 30°C under constant shaking. At 0 and 16 h, the cells were harvested and prepared according to Kuang et al . Using ICP‐AES (the Leeman Prodigy inductively coupled plasma atomic emission spectroscopy, Lowel, MA), intracellular Cd concentrations were detected.…”

Section: Methodsmentioning

confidence: 99%

Effect of cadmium on mRNA mistranslation in Saccharomyces cerevisiae

et al. 2020

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Although highly accurate molecular processes and various messenger RNA (mRNA) quality control and ribosome proofreading mechanisms are used by organisms to transcribe their genes and maintain the fidelity of genetic information, errors are inherent in all biological systems. Low‐level translation errors caused by an imbalance of homologous and nonhomologous amino acids caused by stress conditions are particularly common. Paradoxically, advantageous phenotypic diversity can be generated by such errors in eukaryotes through unknown molecular processes. Here, we found that the significant cadmium‐resistant phenotype was correlated with an increased mistranslation rate of the mRNA in Saccharomyces cerevisiae. This phenotypic change was also related to endogenous sulfur amino acid starvation. Compared with the control, the mistranslation rate caused by cadmium was significantly increased (p < .01). With the increase of cysteine contents in medium, the mistranslation rate of WT(BY4742a) decreased significantly (p < .01). This demonstrates that cadmium treatment and sulfur amino acid starvation both can induce translation errors. Although cadmium uptake is independent of the Sul1 transporter, cadmium‐induced mRNA mistranslation is dependent on the sulfate uptake of the Sul1p transporter. Furthermore, cadmium‐induced translation errors depend on methionine biosynthesis. Taken together, cadmium causes endogenous sulfur starvation, leading to an increase in the mRNA mistranslation, which contributes to the resistance of yeast cells to cadmium. We provide a new pathway mediating the toxicity of cadmium, and we propose that altering mRNA mistranslation may portray a different form of environmental adaptation.

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Effects of cadmium on intracellular cation homoeostasis in the yeastSaccharomyces cerevisiae

Cited by 14 publications

References 31 publications

Associations between blood cadmium levels and cognitive function in a cross-sectional study of US adults aged 60 years or older

Associations between blood cadmium levels and cognitive function in a cross-sectional study of US adults aged 60 years or older

Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae

Effect of cadmium on mRNA mistranslation in Saccharomyces cerevisiae

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