Effects of cadmium and cobalt mixtures on growth and photosynthesis of Raphidocelis subcapitata (Chlorophyceae)

Reis, Larissa Luiza dos; Alho, Lays de Oliveira Gonçalves; Abreu, Cínthia Bruno de; Gebara, Renan Castelhano; Mansano, Adrislaine da Silva; Melão, Maria da Graça Gama

doi:10.1016/j.aquatox.2022.106077

Cited by 12 publications

(8 citation statements)

References 58 publications

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“…An analysis of dissolved metals in the solution indicated that phytoplankton had a notable demand for five trace elements in the following order: Fe > Mn > Cu > Ni > Co. The addition of cobalt at high concentrations resulted in the suppression of algal biomass [ 51 , 52 ], presumably because of the combined effects of cobalt and other metals, such as iron, manganese, and copper. The addition of cobalt at high concentrations resulted in the suppression of the algal biomass.…”

Section: Discussionmentioning

confidence: 99%

Ecotoxicology of Polymetallic Nodule Seabed Mining: The Effects of Cobalt and Nickel on Phytoplankton Growth and Pigment Concentration

Ou,

Huang,

et al. 2023

Toxics

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In order to improve the understanding of the environmental impacts of polymetallic nodule mining, ecotoxicological studies were conducted on the growth of model phytoplankton species Skeletonema costatum and Prorocentrum donghaiense using cobalt and nickel. This study evaluated various physiological and ecological indicators, such as cell proliferation, chlorophyll a, pigments, total protein, and antioxidant enzyme markers. The results show that the introduction of low amounts of cobalt or nickel increased the growth rate of phytoplankton. The phytoplankton benefited from low concentrations of cobalt and nickel stress. The increased protein levels and decreased activity of antioxidant enzymes considerably impacted physiological responses during the promotion of cell abundance. High concentrations of cobalt or nickel resulted in decreased light-absorbing pigments, increased photoprotective pigments, an inactive chlorophyll content, decreased total proteins, and maximal antioxidant enzyme activity in phytoplankton. Throughout the experiment, both the phytoplankton protein and enzyme activity declined with prolonged stress, and the cells underwent age-induced damage. Thus, seabed mining’s repercussions on phytoplankton could result in both short-term growth promotion and long-term damage. These consequences depend on the impurity concentrations infiltrating the water, their duration, and the organism’s physiological responses.

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Section: Discussionmentioning

confidence: 99%

Ecotoxicology of Polymetallic Nodule Seabed Mining: The Effects of Cobalt and Nickel on Phytoplankton Growth and Pigment Concentration

Ou,

Huang,

et al. 2023

Toxics

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“…For this purpose, different cellular and sub-cellular targets/endpoints have been considered (Fig. 4 ), such as (1) size/biovolume and shape (Machado and Soares 2014 , 2020 ; Ostovich et al 2023 ; McKeel et al 2024 ); (2) membrane integrity (viability) (Nagai et al 2011 ; Machado and Soares 2012a , 2015b ); (3) metabolic (esterase) activity (Debenest et al 2012 ; Machado and Soares 2013 ; Peng et al 2019 ; Ciccia et al 2023 ); (4) macromolecules content and distribution: proteins, carbohydrates, and lipids (Moreira et al 2020 ; Ciccia et al 2023 ; Ostovich et al 2023 ); (5) photosynthetic performance and pigment content (chl a , chl b , and carotenoids) (Debenest et al 2012 ; Peng et al 2019 ; Almeida et al 2021 ; dos Reis et al 2022 ); (6) cell cycle and DNA content (Machado and Soares 2014 ; Sousa et al 2018 ; Almeida et al 2019 , 2021 ); (7) mitochondrial membrane potential (Machado and Soares 2015a ; Almeida et al 2019 ; Ciccia et al 2023 ); (8) oxidative stress assessment: ROS production, enzymatic (superoxide dismutase and catalase activity) and non-enzymatic (reduced glutathione) defenses and lipid peroxidation (Machado and Soares 2012b , 2021b ; Almeida et al 2019 ; Filová et al 2021 ); and (9) molecular (transcriptomic and metabolomics) analysis (Guo et al 2021 ; Mizukami-Murata et al 2021 ; Gómez-Martínez et al 2023 ).…”

Section: Use Of R Subcapitata In Aquatic Toxicologymentioning

confidence: 99%

Features of the microalga Raphidocelis subcapitata: physiology and applications

Machado,

Soares

2024

Appl Microbiol Biotechnol

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The microalga Raphidocelis subcapitata was isolated from the Nitelva River (Norway) and subsequently deposited in the collection of the Norwegian Institute of Water Research as “Selenastrum capricornutum Printz”. This freshwater microalga, also known as Pseudokirchneriella subcapitata, acquired much of its notoriety due to its high sensitivity to different chemical species, which makes it recommended by different international organizations for the assessment of ecotoxicity. However, outside this scope, R. subcapitata continues to be little explored. This review aims to shed light on a microalga that, despite its popularity, continues to be an “illustrious” unknown in many ways. Therefore, R. subcapitata taxonomy, phylogeny, shape, size/biovolume, cell ultra-structure, and reproduction are reviewed. The nutritional and cultural conditions, chronological aging, and maintenance and preservation of the alga are summarized and critically discussed. Applications of R. subcapitata, such as its use in aquatic toxicology (ecotoxicity assessment and elucidation of adverse toxic outcome pathways) are presented. Furthermore, the latest advances in the use of this alga in biotechnology, namely in the bioremediation of effluents and the production of value-added biomolecules and biofuels, are highlighted. To end, a perspective regarding the future exploitation of R. subcapitata potentialities, in a modern concept of biorefinery, is outlined. Graphical Abstract Key points • An overview of alga phylogeny and physiology is critically reviewed. • Advances in alga nutrition, cultural conditions, and chronological aging are presented. • Its use in aquatic toxicology and biotechnology is highlighted.

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“…Al + Zn [49,50] As + Se [16] Cu + Cr [74] Cd + Co [52] Cd + Ca [70] Cd + Co [77]-low Cd and high Co Zn + P [75] Cd + Cu + Fe + Mn [52] Cd + Co [53] Cd + Cr [54] Zn + Pb [65] Cd + Cu + Ni + Zn [80] Cd + Co [77]-high Cd and low Co Cd + Cu [29,33,53,54,57,58,60,61] Cu + Ni [69] Cd + Cu [31,56,59] Cd + Pb [65] Cu + Ni + Zn [80] Cd + Zn [24,29,52,66] Cd + Zn [65] Cu + Ti + Zn (nanoparticles) [80] Cd + Co + Cu [53] Cd + Cu + Ni + Zn [78] Cd + Cr + Cu [78] Cu + Co [53] Cd + Ni + Zn [78] Cu + Zn [19] Cd + Co + Fe + Zn + P [83] Cu + Cr + Ni [51] Cu + Fe [68] Cu + Pb + Zn [81]-for growth Cu + Pb [71] Cu + Zn [29,33] Cu + Pb + Zn …”

Section: Synergism Additive Effect Non-interactivementioning

confidence: 99%

“…The presence of Hg/Cu/Zn in a sensitive strain of Chlorella sorokiniana represents competition in the synthesis of metallothionein, even detecting a greater increase in them [114]. Cd and Co induced growth and photosynthetic inhibition in Raphidocelis subcapitata [77]. Antagonism occurs with Cd and Co because they probably compete for the same transport sites on the membrane, since they are bivalent metals [77].…”

Section: Synergism Additive Effect Non-interactivementioning

confidence: 99%

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Reciprocal Effects of Metal Mixtures on Phytoplankton

Nawaz,

Šotek,

Molnárová

2024

Phycology

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Several types of contaminants are anthropogenically introduced into natural aquatic ecosystems and interact with other chemicals and/or with living organisms. Although metal toxicity alone has been relatively well studied, the toxic metal ion effects in the mixture have been thoroughly studied only during the last decades. This review focuses on the published reciprocal effects of different metals on different species of algae, together with describing their toxic effects on studied parameters. Phytoplankton as a bioindicator can help to estimate the reciprocal metal risk factor. Many methodologies have been developed and explored, such as the biotic ligand model (BLM), concentration addition (CA), independent action (IA), sensitivity distribution of EC50 species sensitivity distribution (SSD curves), and others, to study reciprocal metal toxicity and provide promising results, which are briefly mentioned too. From our review, we can commonly conclude the following: Zn acted antagonistically with most heavy metals (Al, Cu, Cd, and Ni). The Cu interaction with Cd, Fe, and Pb was mostly antagonistic. Cd showed synergistic behaviour with Hg, Cu, Zn, and Pb and antagonistic behaviour with Co and Fe in many cases. Methods and techniques need to be developed and optimised to determine reciprocal metal toxicity so that the ecotoxicological predictions made by using phytoplankton can be more accurate and related to real-time toxic metals risks to the aquatic ecosystem. This is the main objective of ecotoxicological tests for risk assessment. Understanding how metals enter algal cells and organelles can help to solve this challenge and was one of the main parts of the review.

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Effects of cadmium and cobalt mixtures on growth and photosynthesis of Raphidocelis subcapitata (Chlorophyceae)

Cited by 12 publications

References 58 publications

Ecotoxicology of Polymetallic Nodule Seabed Mining: The Effects of Cobalt and Nickel on Phytoplankton Growth and Pigment Concentration

Ecotoxicology of Polymetallic Nodule Seabed Mining: The Effects of Cobalt and Nickel on Phytoplankton Growth and Pigment Concentration

Features of the microalga Raphidocelis subcapitata: physiology and applications

Reciprocal Effects of Metal Mixtures on Phytoplankton

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