Effects of Ca2+ on in vitro pollen germination of three Acacia species

Zhan, Nan; LieJian, Huang

doi:10.1515/sg-2016-0012

Cited by 3 publications

(4 citation statements)

References 11 publications

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“…Studies have shown that when SI occurs, the increase in the Ca 2+ concentration leads to the inhibition of pollen tube growth [13,65], and the increase in Ca 2+ is related to the SI response [66,68]. A study of A. mangium showed that high concentrations of Ca 2+ exert a significant inhibitory effect on pollen germination [69]. Our study also identified 28 calcium-dependent protein kinase genes that were significantly differentially expressed.…”

Section: Transcriptomic Analysismentioning

confidence: 51%

“…Our previous research on the pollen vitality of A. mangium has proven the vitality of fresh pollen [44]. Less than 6.67% of polyad-pistil combinations resulted in the germination of the grains after self-pollination or hand-controlled cross-pollination (Table S1), and others (over 90% of the pollen grains) produced no pollen tube growth on the stigma/style (Table S1, Figure 1A).…”

Section: Microscopy Observation Of Pollen Germination and Stigma Cont...mentioning

confidence: 91%

“…Fresh pollen was collected and then stored in the refrigerator for later use [44,45]. After cutting the flowering branches that were strong and about to bloom, the flowering branch watering method (5 leaves, twigs, and 3 g/L potassium dihydrogen phosphate nutrient solution) established by our research group was used for cultivation [43].…”

Section: Site Description and Samplesmentioning

confidence: 99%

“…If we spend a lot of time peeling stamens, this will also strongly affect the experimental results. Moreover, our previous research showed that leaving the stamens unpeeled did not affect the final pollination results [46]. Therefore, the samples used for transcriptomic and metabolomic analyses were derived from intact flowers.…”

Section: Total Rna Extraction and Rna-seq Analysismentioning

confidence: 99%

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Comparative Physiological, Transcriptomic, and Metabolomic Analyses of Acacia mangium Provide New Insights into Its Molecular Mechanism of Self-Incompatibility

Zhang,

Huang,

Zeng

2023

Forests

Self Cite

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Acacia mangium is well known as a valuable commercial tree species in the Acacia genus. A. mangium was recently found to be self-incompatible (SI), but its SI mechanism is not clear, which has hindered the progress of genetic improvement of A. mangium with strong resistance. To confirm the SI type of A. mangium, pollen germination was observed via fluorescence microscopy at 0 h, 3 h, 6 h, 9 h, 12 h, and 24 h after self-pollination. We found over ninety percent of the pollen grains produced no pollen tube growth on the stigma/style. To further explore the SI molecular mechanism of A. mangium, tests of the transcriptome and metabolome were carried out after self-pollination. Observations of pollen germination after self-pollination using fluorescence microscopy suggested that the SI type of A. mangium is gametophytic self-incompatibility (GSI). A combined transcriptomic and metabolomic analysis showed that DEGs (differentially expressed genes) related to SI (6 S-glycoproteins, 93 F-box proteins, 69 26S proteasomes, 38 calcium-dependent protein kinases/calmodulin and 41 thioredoxin genes) were significantly enriched in six KEGG (sulfur metabolism, tyrosine metabolism, phenylalanine metabolism, butanoate metabolism, and valine, leucine, and isoleucine degradation). Further analysis of these six pathways revealed the enrichment of SI-related DEGs corresponding to succinate, methylmalonate, and 3-hydroxypropane. These three metabolites were significantly downregulated. The analysis of transcripts and metabolites suggested that transcripts of SI-related gene families (thioredoxin and F-box protein) were significantly upregulated under the regulation of transcription factors (TFs) after self-pollination, leading to a decrease in metabolites (such as succinate, methylmalonate, and 3-hydroxypropionate). We also further speculated that TFs (MYB, HB-HD-ZIP, AP2/ERF-ERF, and bZIP) and gene families (thioredoxin and F-box protein) were important factors related to the SI of A. mangium.

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