Effects of Ca(OH)2 treatments (“overliming”) on the composition and toxicity of bagasse hemicellulose hydrolysates

Martı́nez, Alfredo; Rodríguez, Martha Eugenia; York, Sean W.; Preston, James F.; Ingram, L. O.

doi:10.1002/1097-0290(20000905)69:5<526::aid-bit7>3.0.co;2-e

Cited by 271 publications

(161 citation statements)

References 37 publications

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“…The concentration of xylose and glucose was decreased at pH11, especially when higher temperature (60 ℃) was applied. Xylose was more destroyed than glucose; this agreed with Martinez's results [18] in which pentose sugars were less stable than hexose sugars when pH was increased from 9 to 11. The concentration of furfural was reduced by treatment with Ca(OH) 2 .…”

Section: Overliming Of the Acid Hydrolyzatesupporting

confidence: 90%

“…However, more researchers investigated detoxification methods to detoxify the hydrolyzate to improve fermentation efficiency. Detoxification methods include neutralization [17], overliming [18], steam stripping [19], ion-exchange [20], treatment with activated carbon [20], wood charcoal [21], and laccase and peroxidase [22]. In many instances, the most economical and widely used method of detoxification involves treatment of hydrolyzates with solid calcium hydroxide [23].…”

Section: Introductionmentioning

confidence: 99%

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Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Zhu¹,

Yong²,

Xu³

et al. 2009

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Yeast Pichia stipitis CBS 5776 was developed through adaptation fermentation step by step in steam exploded corn stover prehydrolyzate because high concentration of weak acids and other inhibitors present in the prehydrolyzate could degrade the fermentability. However, the adaptability of Pichia stipitis CBS 5776 in the prehydrolyzate was so limited that steam stripping and overliming were applied to remove these inhibitors from it. Corn stover was steam exploded; the filtrate of steam exploded corn stover was hydrolyzed with dilute sulfuric acid, and then the acid hydrolyzate was detoxified and fermented by Pichia stipitis CBS 5776. Steam stripping could remove volatile compounds from the acid hydrolyzate and the filtrate. At a steam stripping time of 120min, 81% acetic acid and 59% formic acid were removed from the acid hydrolyzate, 77% acetic acid and 45% formic acid were removed from the filtrate, while furfural was stripped off completely from the acid hydrolyzate and the filtrate. Overliming could reduce the contents of furfural and phenolics present in the acid hydrolyzate, however, sugars, especially pentoses, were also removed partially. It was necessary to detoxify the acid hydrolyzate in order to ferment the sugars to ethanol. Acid hydrolyzate detoxified with a combination of steam stripping for 120 min and overliming at pH11 and 60℃ for 90 min, its fermentability was significantly improved. Xylose was consumed nearly completely in 24h with an ethanol yield of 15.92g/l, 80.34% of theoretical.

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Section: Overliming Of the Acid Hydrolyzatesupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Zhu¹,

Yong²,

Xu³

et al. 2009

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“…The pH of the concentrated hydrolysate was slightly decreased. These results are in well accordance with the studies of Martiniano et al [17] and Milessi et al [18]. Vacuum concentration process is essential to increase the concentration of sugars in the lignocellulosic hydrolysates [19].…”

Section: Discussionsupporting

confidence: 92%

Screening of Yeasts for Selection of Potential Strains and Their Utilization for In Situ Microbial Detoxification (ISMD) of Sugarcane Bagasse Hemicellulosic Hydrolysate

et al. 2016

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Many toxic compounds are produced and released in the hemicellulosic hydrolyzates during the acid pretreatment step, which are required for the disruption of the lignocelluloses matrix and sugars release. The conventional methods of detoxification i.e. overliming, activated charcoal, ion exchange or even membrane-based separations have the limitations in removal of these toxic inhibitors in fermentation process. Hence, it is imperative to explore biological methods to overcome the inhibitors by minimizing the filtration steps, sugar loss and chemical additions. In the present study we screened sixty-four strains of yeasts to select potential strains for detoxification of furfural, acetic acid, ferulic acid, 5-hydroxymethyl furfural (5-HMF) as carbon and energy source. Among these strains Pichia occidentalis M1, Y1 0 a, Y1 0 b and Y3 0 showed a significant decrease in the toxic compounds but we selected two best yeast strains i.e. P. occidentalis Y1 0 a and P. occidentalis M1 for the further experiments with an aim to remove the fermentation inhibitors. The yeasts P. occidentalis Y1 0 a and P. occidentalis M1 were grown aerobically in sugarcane bagasse hemicellulose hydrolysate under submerged cultivation. For each yeast, a 2 2 full factorial design was performed considering the variables-pH (4.0 or 5.0) and agitation rate (100 or 300 rpm), and the percentage removal of HMF, furfural, acetic acid and phenols from hemicellulosic hydrolysates were responsive variables. After 96 h of biological treatment, P. occidentalis M1 and P. occidentalis Y1 0 a showed 42.89 and 46.04 % cumulative removal of inhibitors, respectively.

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“…1, 3, and 4). Efficient detoxification methods are therefore required to improve the utility of lignocellulosic hydrolysate as a fermentation feedstock (1,5,6).…”

mentioning

confidence: 99%

Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14

Koopman

Wierckx

Winde

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

221

240

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The toxic fermentation inhibitors in lignocellulosic hydrolysates pose significant problems for the production of second-generation biofuels and biochemicals. Among these inhibitors, 5-(hydroxymethyl)furfural (HMF) and furfural are specifically notorious. In this study, we describe the complete molecular identification and characterization of the pathway by which Cupriavidus basilensis HMF14 metabolizes HMF and furfural. The identification of this pathway enabled the construction of an HMF and furfural-metabolizing Pseudomonas putida. The genetic information obtained furthermore enabled us to predict the HMF and furfural degrading capabilities of sequenced bacterial species that had not previously been connected to furanic aldehyde metabolism. These results pave the way for in situ detoxification of lignocellulosic hydrolysates, which is a major step toward improved efficiency of utilization of lignocellulosic feedstock.

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Effects of Ca(OH)2 treatments (“overliming”) on the composition and toxicity of bagasse hemicellulose hydrolysates

Cited by 271 publications

References 37 publications

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Screening of Yeasts for Selection of Potential Strains and Their Utilization for In Situ Microbial Detoxification (ISMD) of Sugarcane Bagasse Hemicellulosic Hydrolysate

Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14

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