Effects of C-Myc Antisense Transcripts on Differentiation of K562 Cells

Yang, Yw; Yang, MC

doi:10.3892/ijo.6.2.419

Cited by 2 publications

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“…Hereinto, the part of the dissolved organics was further oxidized to small molecular substances . The concentrations of protein and polysaccharide were used as an index to measure the degree of EPS degradation . The Fenton reaction system generated hydroxyl radicals (•OH) with the existence of H 2 O 2 and the catalysis of Fe 2+ under acidic conditions, and the amount of Fe 2+ and H 2 O 2 directly influenced the production of •OH .…”

Section: Resultsmentioning

confidence: 99%

Effective sludge dewatering technique using the combination of Fenton's reagent and CPAM

et al. 2017

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In this study, the combination of Fenton's reagent and cationic polyacrylamide (CPAM) was used in sludge conditioning for the enhancement of sludge dewatering performance. The effects of CPAM, H2O2, and Fe2+ dosages and pH on the moisture content (MC) of the filter cake, the specific resistance of filtration (SRF), and the residual turbidity of the supernatant (RT) were investigated. To observe influencing mechanisms of sludge dewatering further, optical microscopy and scanning electron microscopy (SEM) were employed. Results demonstrated that the sludge dewatering performance obtained by the combination of Fenton's reagent and CPAM was significantly better than that obtained using Fenton's reagent or CPAM alone. The optimum conditions of sludge conditioning were as follows: 400 mg · L−1 Fe2+, 4 g · L−1 H2O2, 40 mg · L−1 CPAM, and pH 4. The SRF, MC, and RT at optimum conditions were reduced to minimum values of 1.06 × 1012 m/kg, 58.9 %, and 3.7, respectively. The optical microscopy and SEM analyses of sludge flocs confirmed that they were more conducive to sludge dewatering after the combination of Fenton oxidation and flocculation. The sludge dewatering results demonstrated that the combination of Fenton and flocculation process exhibited excellent performance in enhancing sludge dewatering and is a promising pretreatment approach to sludge disposal.

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Section: Resultsmentioning

confidence: 99%

Effective sludge dewatering technique using the combination of Fenton's reagent and CPAM

et al. 2017

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“…During erythroid and myelomonocytic differentiation, c-MYC plays a critical role. The overexpression of c-MYC leads to the partial inhibition of erythroid differentiation in K562 cells (18)(19)(20)(21). In addition, c-MYC suppresses the differentiation induced by imatinib in chronic myeloid leukemia cells (22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

ITR‑284 modulates cell differentiation in human chronic myelogenous leukemia K562 cells

et al. 2017

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ITR‑284 is a carboxamide analog that can inhibit proliferation in human promyelocytic leukemia HL-60 cells. To understand the effects and molecular mechanisms of ITR‑284 in human erythromyeloblastoid leukemia, we treated K562 cells with different concentrations of ITR‑284 (0, 2, 4, 6, 8 and 10 nM) and all-trans retinoic acid (ATRA) (0, 0.1, 0.5, 1, 5 and 10 µM) for 24 h. The IC50 of ITR‑284 was ~10 nM in K562 cells treated for 24 h as determined by MTT assay. May-Grünwald-Giemsa staining and nitro blue tetrazolium (NBT) assays were used to determine cell morphology changes and differentiation after ITR‑284 and ATRA treatment. In addition, mRNA expression levels of hematopoietic factors, including GATA‑1, NF-E2 and GATA‑2, were elevated, while expression levels of BCR‑ABL were downregulated in K562 cells after 24 h of treatment with ITR‑284 as determined by quantitative reverse transcription polymerase chain reaction. In addition, western blot analyses showed that FOXM1, GLI 1 and c-MYC protein levels were decreased by ITR‑284. Taken together, our data show that ITR‑284 induced K562 cell differentiation, which led to decreased tumorigenesis. Our findings suggest that ITR‑284 could be a potential candidate for treating chronic myelogenous leukemia.

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Effects of C-Myc Antisense Transcripts on Differentiation of K562 Cells

Cited by 2 publications

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Effective sludge dewatering technique using the combination of Fenton's reagent and CPAM

Effective sludge dewatering technique using the combination of Fenton's reagent and CPAM

ITR‑284 modulates cell differentiation in human chronic myelogenous leukemia K562 cells

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