Effects of butyric acid, a bacterial metabolite, on the migration of ameloblastoma mediated by laminin 332

Abstract

Ameloblastoma is a benign tumor that develops in the jawbone. Occasionally, however, it may become malignant and metastasize to other tissues. Although it has been suggested that various cytokines and several adhesion factors may play a role in its malignant transformation, the details have not been elucidated. In this context, it has been reported that butyric acid produced by periodontopathic bacteria causes progression of malignant tumors occurring in the mouth via podoplanin. However, the influence of butyric acid on ameloblastoma has not been clarified. In the present study, therefore, the expression of various cytokines and adhesion factors in ameloblastoma upon stimulation with butyric acid or cytokines was investigated using real-time reverse-transcription polymerase chain reaction. Three cell lines (HAM1, HAM2 and HAM3) established from the same ameloblastoma were used in the experiments. It was found that the expression of mRNAs for epidermal growth factor (EGF) and transforming growth factor beta 1 (TGFβ1) was increased in HAM2 and HAM3, respectively, upon stimulation with butyric acid. In addition, stimulation with EGF and TGFβ1 led to an increase in the expression of laminin β-3 mRNA in the respective cell lines. These results suggest that butyric acid may be involved in ameloblastoma exacerbation through the expression of laminin 332 (LM332) via EGF and TGFβ1 produced by ameloblastoma itself.

Uptake of Nanotitania by Gingival Epithelial Cells Promotes Inflammatory Response and Is Accelerated by Porphyromonas gingivalis Lipopolysaccharide: An In Vitro Study

“…qPCR was performed as previously described [ 48 ]. Briefly, we used the StepOne Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.…”

Oral Microbiota-Driven Cell Migration in Carcinogenesis and Metastasis

“…Large amounts of BA have been detected in the oral cavities of patients with periodontal disease. BA from periodontopathic bacteria does not directly affect the migration of ameloblastomas but has an indirect influence through the expression of epidermal growth factor (EGF) and transforming growth factor b1 (TGF-b1) (Ishikawa et al, 2020). Other oral bacteria are also related to cell migration.…”

The Multifaceted Roles of Diet, Microbes, and Metabolites in Cancer

“…Valproic acid, a histone deacetylase inhibitor, has recently been demonstrated to inhibit growth of glioma cells in models of high-grade gliomas [113]. In contrast to the highlighted positive effects of SCFAs, the bacterial metabolite butyric acid plays a role in exacerbating ameloblastoma, a benign tumour of the jawbone, through interactions with epidermal growth factor and transforming growth factor β1 secreted by the tumour cells [114].…”