Effects of Butyrate and Dibutyryl Cyclic AMP on hCG-secreting Trophoblastic and Non-trophoblastic Cells*

Hussa, Robert O.; Pattillo, Roland A.; Rückert, A; Scheuermann, Keith W.

doi:10.1210/jcem-46-1-69

Cited by 91 publications

(25 citation statements)

References 12 publications

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“…Further, cell-specific responses of the endogenous at gene to cAMP and butyrate parallel to those obtained with transfected a sequences have been reported at the protein level in eutopic cells (Fig. 3) (7, 8, 21-23, 36) and ectopic cells (7,10,12,23) and at the transcriptional level in ectopic cells (10). Thus, regulation of the transfected paCAT constructs appears to be a valid reflection of regulation of the endogenous a gene.…”

supporting

confidence: 66%

“…In ectopic and eutopic cell lines, a expression appears to respond differently to cyclic AMP (cAMP) and sodium butyrate. For example, cAMP induces a protein synthesis in eutopic cell lines (7,(21)(22)(23), but has little or no effect on a protein levels in * Corresponding author. ectopic cells (21; unpublished data).…”

mentioning

confidence: 99%

“…ectopic cells (21; unpublished data). Conversely, butyrate stimulates a transcription in HeLa cells but has no apparent effect on a protein levels in eutopic cells (8,10,23,36).…”

mentioning

confidence: 99%

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Differential expression of the human gonadotropin alpha gene in ectopic and eutopic cells.

Darnell¹,

Boime²

1985

Mol. Cell. Biol.

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supporting

confidence: 66%

mentioning

confidence: 99%

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Differential expression of the human gonadotropin alpha gene in ectopic and eutopic cells.

Darnell¹,

Boime²

1985

Mol. Cell. Biol.

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“…Others were derived from secondary deposits. These include CaSki, ME-180, MS751, HT-3, EC50 and DoT (Sykes et al, 1970;Fogh & Trempe, 1975;Patillo et al, 1977;Hussa et al, 1978;Porter et al, 1978). The W12 cell line was established from a CIN 1 cervical lesion (Stanley et al, 1989).…”

Section: Discussionmentioning

confidence: 99%

XH1 – a new cervical carcinoma cell line and xenograft model of tumour invasion, 'metastasis' and regression

Han

Lyle²,

Eustace³

et al. 1991

Br J Cancer

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“…JAr, JEG-3) and nontrophoblastic (e.g. HeLa, ChaGo, CBT) tumors (16,18). In the latter instance, they are considered to be ectopic proteins, i.e.…”

mentioning

confidence: 99%

Mechanism of Gonadotropin Gene Expression

Xiong

Tapprich

Cox

2002

Journal of Biological Chemistry

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Regulation of the glycoprotein hormone ␣-subunit (GPH␣) gene has been studied extensively in pituitary and placental cell lines, but little is known of the transcriptional regulators important for its ectopic expression. To investigate the molecular basis for ectopic expression, it was critical to define cis-regulatory elements and their cognate trans-acting factors that modulate promoter activity in epithelial cell types that do not normally express GPH. DNA-mediated transient expression of promoter-reporter constructs was used to identify a novel negative regulatory element located at the GPH␣ gene transcription start site. Truncation or site-directed mutagenesis of this element produced up to a 10-fold increase in promoter activity. Electrophoretic mobility shift analysis detected a protein that binds specifically to a DNA motif encompassing the cap site. Based on competitive DNA binding studies with mutated oligonucleotides, it was determined that bases from ؊5 to ؊2 and ؉4 to ؉11 are critical for protein binding. The DNA sequence flanking the transcription start site from ؊9 to ؉11 is an imperfect palindrome; consequently, this motif is referred to as the cap site diad element (CSDE) and the cognate factor as the cap site-binding protein (CSBP). CSBP activity was present at different levels in nuclear extracts prepared from a variety of cell types. Significantly, the ratio of activities exhibited by the GPH␣ promoter with a mutated CSDE compared with the promoter with a wild-type CSDE was dependent on the transfected cell line and its content of CSBP. These results indicate that a negative regulatory element centered at the GPH␣ gene cap site and its cognate DNA-binding protein make a significant contribution to the production of ␣-subunit in a variety of tumor tissues. A detailed understanding of this cis/trans pair may further suggest a mechanism to explain, at least in part, how this gene becomes activated in nonendocrine tumors.

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Effects of Butyrate and Dibutyryl Cyclic AMP on hCG-secreting Trophoblastic and Non-trophoblastic Cells*

Cited by 91 publications

References 12 publications

Differential expression of the human gonadotropin alpha gene in ectopic and eutopic cells.

Differential expression of the human gonadotropin alpha gene in ectopic and eutopic cells.

XH1 – a new cervical carcinoma cell line and xenograft model of tumour invasion, 'metastasis' and regression

Mechanism of Gonadotropin Gene Expression

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