ABSTRACT:Data from 662 litters and 221 samples (two replicates per each sample) of rabbit milk were collected from three different genetic groups [Gabali (G), V-line (V) and G×V (F 1 )] to study the effect of line and of some non-genetic factors affecting production and milk composition traits. The studied traits were: number born alive (LSBA); litter size at weaning (LSW); litter weight born alive (LWBA); litter weight at weaning (LWW); total milk yield (TMY) and protein, fat, total solids (TS), ash, phosphorus (P), potassium (K), sodium (Na), calcium (Ca) and magnesium (Mg) milk content. The genetic group effect was significant for LSBA [V (7.3) and F 1 (7.3) were superior to G (6.6)); LWBA (higher values for V (416 g) and F 1 (405 g) than for G (382 g)]; TMY [G (3497 g) and F 1 (3486 g) higher than V (3042 g)]; milk fat [F 1 (26%) and G (25%) superior to V (23%)] and milkTS [F 1 (42%) superior to G (40%) and V (39%)]. The estimates of the heterosis effect ranged between 1.6% and 12.8% for production and milk gross chemical traits, being significant only for milk fat content (9.1%). The positive values of the heterosis estimates for those traits and the complementarity between the G breed and the V line, the G superior in milk related traits but the V superior in prolificacy, show the interest of their cross and of the synthetic lines derived from them. The heterosis was non-significant for milk mineral content traits. The parity effects were significant for LSBA, fat, TS, ash and K (the maxima were reached at 6 th or 5 th parity). The effect of week of lactation was significant for fat, ash and TS, corresponding the maximum values of fat and TS to the 3 rd and 4 th week. The year-season or the year-month effects were significant for all traits showing the importance of including these time-period factors into the statistical models proposed for the traits.