Effects of borneol on the level of DNA damage induced in primary rat hepatocytes and testicular cells by hydrogen peroxide

Horváthová, Eva; Slameñová, D; Maršálková, Lenka; Šrámková, Monika; Wsólová, Ladislava

doi:10.1016/j.fct.2009.03.002

Cited by 43 publications

(37 citation statements)

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“…It is commonly accepted that due to oxidative stress, reactive oxygen species (ROS) such as superoxid, hydroxyl and peroxyl radicals are generated [1]. Recently much attention has been given to the natural phytochemicals with antioxidant, antimutagenic and anticarcinogenic activities [2][3][4][5][6][7][8]. These compounds are of great importance for chemoprevention and potential cancer biotherapy.…”

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confidence: 99%

Biological activity of plant extract isolated from Papaver rhoeas on human lymfoblastoid cell line

Hašplová¹,

Hudecová²,

Miadoková³

et al. 2011

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Varied medicinal plants are known as a source of natural phytochemicals with antioxidant activities that can protect organisms from oxidative stress and from various chronic diseases. Papaver rhoeas has a long history of medicinal usage, especially for ailments in adults and children. The possible cytotoxicity, genotoxicity and potential antioxidant effect of plant extract isolated from flowers of Papaver rhoeas was investigated in human lymfoblastoid cell line (TK6). Antioxidant activity of this extract was determined using the DPPH assay. The plant extract exhibited dose dependent free radical scavenging ability. The growth activity assay was used for determination of cytotoxicity. To assess potential genotoxicity the comet assay was used. The lower extract concentrations (0.25 and 0.5 mg/ml) neither exerted cytotoxic, nor genotoxic effects in TK6 cells but they stimulated cell proliferation. The concentration 25 mg/ml scavenged almost 85% of DPPH free radical. On the other hand, this concentration had strong cytotoxic and genotoxic effect on TK6 cells.The balance between beneficial and harmful effects should be always considered when choosing the effective dose. Plants are universally recognized as a vital part of the world's natural heritage and up to 80% of the population relies on plants for their contribution to a primary healthcare. The use of plants in traditional medicine is widespread. Many plants still represent a large source of natural antioxidants that might serve as leads for the development of novel drugs. It is commonly accepted that due to oxidative stress, reactive oxygen species (ROS) such as superoxid, hydroxyl and peroxyl radicals are generated [1]. Recently much attention has been given to the natural phytochemicals with antioxidant, antimutagenic and anticarcinogenic activities [2][3][4][5][6][7][8]. These compounds are of great importance for chemoprevention and potential cancer biotherapy.Papaveraceae is a family of flowering plants. The flowers of poppy are chiefly employed as a mild pain reliever and as a treatment for irritable coughs; it also helps to reduce nervous over-activity [9]. The flowers and petals are anodyne, expectorant, hypnotic, slightly narcotic and sedative. An infusion is taken internally in the treatment of bronchial complaints and coughs, insomnia, poor digestion, nervous digestive disorders and minor painful conditions. The flowers are also used in the treatment of jaundice and -in very small quantities-as a sleep-inducing drug [9,10].Papaver rhoeas plant extract contains a lot of flavonoids: kaempferol, quercetin, luteolin and hypolaetin andAntioxidant activity of the kaempferol [12][13], quercetin [14][15] and luteolin [16] was already demonstrated. Total phenolic compounds (9.73 -19.91 mg GAE/g) and total flavonoids (7.904-11.45 mg QE/g) were determined in fresh petals [17].Besides flavonoids and their glycosides Papaveraceae family are known for the presence of alkaloids as morphine, codeine, narcotine, thebaine and papaverine [8,18,19].The aim of this study...

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confidence: 99%

Biological activity of plant extract isolated from Papaver rhoeas on human lymfoblastoid cell line

Hašplová¹,

Hudecová²,

Miadoková³

et al. 2011

neo

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“…Based on this aspect, our results are not contradictory to the results obtained from this study. Horváthová et al (2009) reported that high concentrations of borneol (34.28 mg/kg/day) showed a cytotoxic effect in primary rat hepatocytes due to the increased levels of DNA damage and that this effect resulted probably from the induction of apoptotic and necrotic DNA fragmentation. Furthermore, Bakkali et al (2008) reported that the main mechanisms of cytotoxic effects of essential oils in mammalian cells were apoptosis and necrosis.…”

Section: Discussionmentioning

confidence: 99%

The effects of 4-thujanol on chromosome aberrations, sister chromatid exchanges and micronucleus in human peripheral blood lymphocytes

Kocaman¹,

Rencüzoğulları²,

Topaktaş³

et al. 2011

Cytotechnology

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4-Thujanol, a bicyclic monoterpene alcohol, is present in the essential oils of many medicinal and aromatic plants. It is commonly used as a fragrance and flavouring ingredient in a lot of different products. The potential genotoxic effects of 4-thujanol on human peripheral blood lymphocytes (PBLs) were investigated in vitro by the chromosome aberrations (CAs), sister chromatid exchanges (SCEs), and micronucleus (MN) tests. The cells were treated with 13, 26 and 52 lg/mL 4-thujanol in the presence and absence of a metabolic activator (S9 mix). 4-Thujanol induced CA (P \ 0.001) and MN formation (P \ 0.05) at all concentrations (13, 26 and 52 lg/mL) in the presence and absence of the S9 mix without a concentration-dependent manner. However, the treatment of peripheral lymphocytes with 4-thujanol did not produce a statistical difference in the frequency of SCEs when compared with control group. Furthermore, this monoterpene did not significantly decrease the mitotic index (MI), proliferation index (PI), and nuclear division index (NDI). In conclusion, 4-thujanol had a significant clastogenic effect at the tested concentrations (13, 26 and 52 lg/mL) for human PBLs. In addition, no cytotoxic and/or cytostatic effects were observed regardless of the concentrations used. This work presents the first report on genotoxic properties of 4-thujanol.

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“…Antioxidant activity was thought to be one mode of action of Ficus bengalensis extract regarding its neuroprotective effect. A well-built association between vegetable or fruit consumption and protection against oxidative DNA-damage was found in recent comet experiments 16,17 .Some authors demonstrated with supplementation of standard antioxidants like quercetin or borneol and observed significant reduction in oxidative DNA damage against H 2 O 2 challenge 18,19 .…”

Section: Neuronal Protection Of Fb Extracts Against H 2 O 2 -Induced mentioning

confidence: 97%

Protective effect of Ficus bengalensis L. extract against H2O2 induced DNA damage and repair in neuroblastoma cells

et al. 2014

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Effects of borneol on the level of DNA damage induced in primary rat hepatocytes and testicular cells by hydrogen peroxide

Cited by 43 publications

References 40 publications

Biological activity of plant extract isolated from Papaver rhoeas on human lymfoblastoid cell line

Biological activity of plant extract isolated from Papaver rhoeas on human lymfoblastoid cell line

The effects of 4-thujanol on chromosome aberrations, sister chromatid exchanges and micronucleus in human peripheral blood lymphocytes

Protective effect of Ficus bengalensis L. extract against H2O2 induced DNA damage and repair in neuroblastoma cells

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