Effects of bone-seeking hormones on DNA synthesis, cyclic AMP level, and alkaline phosphatase activity in cultured cells from human posterior longitudinal ligament of the spine

Ishida, Yoichiro; Kawai, Shinya

doi:10.1002/jbmr.5650081104

Cited by 30 publications

(5 citation statements)

References 44 publications

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“…6 Thus, recent research on OPLL has involved undertaking association and/or genome-wide linkage analyses 9,12,23 to determine the candidate genes, as well as proteomics analysis for detecting causative peptides in ossifying plaque. Histologically, abnormal enchondral ossification 2,8 may play a role in OPLL, but because of the paucity of histological studies 11,14,16 the underlying mechanisms of calcification and ossification processes remain obscure.…”

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confidence: 99%

Ossification of the posterior longitudinal ligament of the cervical spine: histopathological findings around the calcification and ossification front

Sato

Uchida

Kobayashi

et al. 2007

SPI

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Object. The authors studied the histological and immunohistochemical features of ossified posterior longitudinal ligament (PLL) of the cervical spine, especially in the calcification and ossification front. Methods. Samples of en bloc ossified PLL plaque obtained in 31 patients were stained with H & E and immuno-histochemically prepared for collagens (types I and II), vascular endothelial growth factor (VEGF), transforming growth factor (TGF)–β, and bone morphogenetic protein (BMP)–2, and by terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick-end labeling method for apoptosis. Results. Enchondral ossification was evident between the ligamentous enthesis and deep layer of the ligament, with irregularly disorganized arrangement of elastic fibers in association with advancement of the degenerative process. In the ossification front, many hypertrophic metaplastic chondrocytes were noted in the ossifying plaque immediately contiguous to the ligament fibers, together with a considerable degree of neovascularization. Both TGFβ and BMP-2 were highly expressed in metaplastic hypertrophic chondrocytes in the ossification front, and BMP-2 was also expressed in fibroblastic cells near the ossified PLL plaque. Expression of type I collagen was significant in the matrix of the ossified PLL lesion, whereas that of type II was marked in metaplastic chondrocytes in the ossification front. Apoptotic hypertrophic chondrocytes were observed mainly in the fibrocartilaginous area near the calcification front. Conclusions. The enchondral ossification process in the ossified PLL was closely associated with degenerative changes of elastic fibers and cartilaginous cartilage formation, together with the appearance of metaplastic hypertrophic cartilage cells and neovascularization. The authors also found that VEGF-positive metaplastic chondrocytes in the ossification front and different expression patterns of collagens probably play some role in the extension of the ossified PLL from the ossification front.

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confidence: 99%

Ossification of the posterior longitudinal ligament of the cervical spine: histopathological findings around the calcification and ossification front

Sato

Uchida

Kobayashi

et al. 2007

SPI

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“…Disorders such as hypoparathyroidism, X-linked hypophosphatemia, Paget's disease, vitamin Dresistant rickets, non-insulin-dependent diabetes mellitus, and calcium and fluoride derangements, as well as other spinal ligamentous ossifications. 4,5,20,[34][35][36][37][38][39][40][41][42][43][44][45][46] Finally, it has been suggested that the genetic background may explain the increased prevalence of OLF in certain ethnic and racial groups. [10][11][12] Ossification of the ligamentum flavum is a major cause of myelopathy in Japan.…”

Section: Discussionmentioning

confidence: 99%

Compressive Myelopathy Due to Ossification of the Ligamentum Flavum: Case Report and Review of the Literature

et al. 2017

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Ossification of the ligamentum flavum (OLF) is a rare condition in which the ligamentum flavum, due to mechanical, biological and genetic factors, becomes ossified. Due to its nature and anatomic location, OLF produces symptoms characteristic of spinal cord compression. The diagnostic confirmation is based primarily on imaging tests such as computed tomography (CT) and magnetic resonance imaging (MRI). Ossification of the ligamentum flavum most often affects Asian populations, rarely occurring in black people. The authors report a case of a 61-year-old black man with progressive paraparesis due to OLF, and review the literature regarding the pathology's prevalence, pathogenesis, clinical features, diagnosis, treatment and prognosis.

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Section: Pathophysiology and Risk Factorsmentioning

confidence: 99%

“…5 At the cellular level, the abnormal PLL features bone matrix cells with an osteoblast-like phenotype, including increased alkaline phosphatase activity, parathyroid hormone responsiveness, calcitonin levels, and 1,25(OH) 2 vitamin D levels relative to control subjects. 6 Several comorbidities have also been associated with OPLL. Patients with OPLL have elevated rates of diabetes, cardiac disease, and vascular disease.…”

Section: Pathophysiology and Risk Factorsmentioning

confidence: 99%

Ossification of the Posterior Longitudinal Ligament: Pathophysiology, Diagnosis, and Management

Wick

Van

et al. 2022

J Am Acad Orthop Surg

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Ossification of the posterior longitudinal ligament (OPLL) occurs as heterotopic bone forms in the posterior longitudinal ligament, resulting in neural compression, myelopathy, and radiculopathy. OPLL is most commonly observed in East Asian populations, with prevalence rates of 1.9% to 4.3% reported in Japan. OPLL rates are lower in North American and European patients, with reported prevalence of 0.1% to 1.7%. Patients typically develop symptoms due to OPLL in their cervical spines. The etiology of OPLL is multifactorial, including genetic, metabolic, and anatomic factors. Asymptomatic or symptomatic patients with OPLL can be managed nonsurgically, whereas patients with neurologic symptoms may require surgical decompression from an anterior, posterior, or combined approach. Surgical treatment can provide notable improvement in neurologic function. Surgical decision making accounts for multiple factors, including patient comorbidities, neurologic status, disease morphology, radiographic findings, and procedure complication profiles. In this study, we review OPLL epidemiology and pathophysiology, clinical features, radiographic evaluation, management, and complications. O ssification of the posterior longitudinal ligament (OPLL) occurs as the heterotopic bone replaces the posterior longitudinal ligament (PLL), resulting in varying degrees of spinal stenosis and neurologic dysfunction. OPLL occurs in a 2:1 male-to-female ratio, with variable geographic prevalence. The condition was first described in Japanese and Eastern Asian patients, with reported prevalence in Japan ranging from 1.9% to 4.3% and approaching 3.0% in other Asian countries. 1 Rates are lower in North America and Europe, where prevalence is reported from 0.1% to 1.7%. 2 True prevalence rates may be higher because CT studies in Japan have found cervical OPLL in 6.3% of asymptomatic, healthy subjects, 3 and up to 71% of patients noted to have OPLL were found to remain asymptomatic over a 30year follow-up. 1 When symptoms develop, OPLL patients may experience radiculopathy and myelopathy and sustain spinal cord injury (SCI), typically around the age of 40 to 60 years. Given the risk of neurologic injury associated with OPLL, it

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Effects of bone-seeking hormones on DNA synthesis, cyclic AMP level, and alkaline phosphatase activity in cultured cells from human posterior longitudinal ligament of the spine

Cited by 30 publications

References 44 publications

Ossification of the posterior longitudinal ligament of the cervical spine: histopathological findings around the calcification and ossification front

Ossification of the posterior longitudinal ligament of the cervical spine: histopathological findings around the calcification and ossification front

Compressive Myelopathy Due to Ossification of the Ligamentum Flavum: Case Report and Review of the Literature

Ossification of the Posterior Longitudinal Ligament: Pathophysiology, Diagnosis, and Management

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