Effects of bombesin and insulin on inositol (1,4,5) trisphosphate and inositol (1,3,4) trisphosphate formation in Swiss 3T3 cells

Heslop, John P.; Blakeley, D M; Brown, Kenneth D.; Irvine, Robin F.; Berridge, Michael J.

doi:10.1016/0092-8674(86)90513-1

Cited by 185 publications

(83 citation statements)

References 43 publications

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“…This observation is consistent with the lack of effect of insulin on PIP2 hydrolysis Heslop et al, 1986). In addition, although insulin synergizes with bombesin in stimulating DNA synthesis in Swiss 3T3 cells, insulin failed to potentiate the bombesin-mediated release of 45Ca2+ from the cells (results not shown).…”

Section: Bombesin-stimulated Release Of 45ca2+ From Swiss 3t3 Cellssupporting

confidence: 78%

“…Swiss 3T3 cells, which possess specific high-affinity receptors for radiolabelled gastrin-releasing peptide (Zachary & Rozengurt, 1985) and [Tyr4]bombesin (Brown & Laurie, 1986), should prove useful for this purpose. The binding of bombesin to its receptors on Swiss 3T3 cells results in a rapid stimulation of the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) Heslop et al, 1986), generating inositol 1,4,5-trisphosphate (1,4,5-4P3) and diacylglycerol, an endogenous activator of protein kinase C (see Nishizuka, 1984). Hydrolysis of PIP2 in 3T3 cells is also stimulated by vasopressin , prostaglandin F2, (MacPhee et al, 1984), and Vol.…”

Section: Introductionmentioning

confidence: 99%

“…platelet-derived growth factor Chu et al, 1985), but not by insulin Heslop et al, 1986), insulin-like growth factor 1 (K. D. Brown, unpublished work) or epidermal growth factor (Besterman et al, 1986). The PIP2-hydrolysing agonists induce an 1P3-mediated mobilization of intracellular Ca2+ from bound stores, which can be demonstrated either as an increased efflux of 45Ca2+ from pre-labelled cells Lopez-Rivas & Rozengurt, 1984) or as an elevation of intracellular free Ca2+ concentration measured with quin2 (Hesketh et al, 1985;Mendoza et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

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Protein kinase C-mediated negative-feedback inhibition of unstimulated and bombesin-stimulated polyphosphoinositide hydrolysis in Swiss-mouse 3T3 cells

Brown

Blakeley

Hamon

et al. 1987

Biochemical Journal

113

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Bombesin-related peptides stimulate a rapid increase in polyphosphoinositide hydrolysis in Swiss-mouse 3T3 cells. These peptides generate an increase in the efflux of 45Ca2+ from pre-labelled cells, a response consistent with an inositol trisphosphate-mediated mobilization of intracellular Ca2+. The bombesin-stimulated release of cellular 45Ca2+ is inhibited by tumour-promoting phorbol esters (e.g. 12-0-tetradecanoylphorbol 13-acetate, TPA). Although there are several possible sites of action at which this effect might occur, our results indicate that TPA induces an uncoupling of bombesin-stimulated hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) without decreasing cellular binding of bombesin. In cultured cells, protein kinase C can be down-modulated by a prolonged incubation of the cells with phorbol esters. Such pretreatment greatly decreased the inhibitory effect of TPA on bombesin-stimulated PIP2 hydrolysis, suggesting that this action of the phorbol ester is mediated via protein kinase C. Since diacylglycerol is an endogenous activator of protein kinase C and a direct product of PIP2 hydrolysis, these results suggest that protein kinase C inhibition of polyphosphoinositide hydrolysis may function as a negative-feedback pathway. Cells in which protein kinase C has been down-modulated show elevated basal and bombesin-stimulated production of inositol phosphates, providing evidence that such a feedback loop limits polyphosphoinositide turnover in both unstimulated and mitogen-stimulated cells.

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Section: Bombesin-stimulated Release Of 45ca2+ From Swiss 3t3 Cellssupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Protein kinase C-mediated negative-feedback inhibition of unstimulated and bombesin-stimulated polyphosphoinositide hydrolysis in Swiss-mouse 3T3 cells

Brown

Blakeley

Hamon

et al. 1987

Biochemical Journal

113

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“…Inositol tetrakisphosphates (InsP4) appear to mediate Ca2+ sequestration [4], Ca2+ influx [5,6] and membrane current activities [7,8]. Inositol pentakisphosphates (InsPs) exist in mammalian cell types [6,, but have been considered as a 'housekeeping' inositol phosphate rather than an acute second messenger [2].…”

Section: Introduction 22 Measurement Of Inositol Phosphate Accumulamentioning

confidence: 99%

Rapid increase in inositol pentakisphosphate accumulation by nicotine in cultured adrenal chromaffin cells

1990

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When pH]inositol-prelabeled cultured bovine adrenal chromallin cells were stimulated with nicotine (10 GM), a large and transient increase in PHIinositol pentakisphosphate (InsP,) accmnulation was observed. The accumulation reached the maximum level at 15 s, then declined to the basal level at 2 min. Nicotine also induced PH]inositol tetrakisphosphate (InsP,) and PH]inositol hexakisphosphate (InsP,) accumulation with a slower time course and a lesser magnitude than PH]InsP,. The peaks of PH]InsP,, PH]InsP, and PH]InsP, coincided with those of 3zP radioactivity, when cells were doubly labeled with PH]inositol and inorganic 3zP. These results suggest that inositol pentakisphosphate is rapidly increased by nicotine, a cholinergic agonist, in cultured adrenal chromaffin cells.

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“…The amphibian tetradecapeptide bombesin and structurally related mammalian peptides, including gastrin-releasing peptide (GRP), are potent mitogens for Swiss 3T3 cells (2,3). The peptides bind to high-affinity cell-surface receptors in these cells (3) and elicit a complex array of early biological responses (4), including enhanced inositol phospholipid breakdown and mobilization of Ca2+ from intracellular stores (5)(6)(7), stimulation of Na+/H+ antiport activity (6), activation of protein kinase C (8-10), inhibition of 125I-labeled epidermal growth factor (EGF) binding (8,9,11), and induction of the cellular oncogenes c-fos and c-myc (12)(13)(14). Zachary and Rozengurt (15) identified a surface protein in Swiss 3T3 cells with Mr 75,000-85,000 as a putative component of the bombesin/GRP receptor.…”

mentioning

confidence: 99%

[D-Arg1,D-Phe5,D-Trp7,9,Leu11]substance P, a potent bombesin antagonist in murine Swiss 3T3 cells, inhibits the growth of human small cell lung cancer cells in vitro

1995

Lung Cancer

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Effects of bombesin and insulin on inositol (1,4,5) trisphosphate and inositol (1,3,4) trisphosphate formation in Swiss 3T3 cells

Cited by 185 publications

References 43 publications

Protein kinase C-mediated negative-feedback inhibition of unstimulated and bombesin-stimulated polyphosphoinositide hydrolysis in Swiss-mouse 3T3 cells

Protein kinase C-mediated negative-feedback inhibition of unstimulated and bombesin-stimulated polyphosphoinositide hydrolysis in Swiss-mouse 3T3 cells

Rapid increase in inositol pentakisphosphate accumulation by nicotine in cultured adrenal chromaffin cells

[D-Arg1,D-Phe5,D-Trp7,9,Leu11]substance P, a potent bombesin antagonist in murine Swiss 3T3 cells, inhibits the growth of human small cell lung cancer cells in vitro

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