Effects of BMP-2 and vitamin D3 on the osteogenic differentiation of adipose stem cells

Mishra²,

et al. 2018

IJMS

Adipose-derived stromal/stem cells (ASCs) seems to be a promising regenerative therapeutic agent due to the minimally invasive approach of their harvest and multi-lineage differentiation potential. The harvested adipose tissues are further digested to extract stromal vascular fraction (SVF), which is cultured, and the anchorage-dependent cells are isolated in order to characterize their stemness, surface markers, and multi-differentiation potential. The differentiation potential of ASCs is directed through manipulating culture medium composition with an introduction of growth factors to obtain the desired cell type. ASCs have been widely studied for its regenerative therapeutic solution to neurologic, skin, wound, muscle, bone, and other disorders. These therapeutic outcomes of ASCs are achieved possibly via autocrine and paracrine effects of their secretome comprising of cytokines, extracellular proteins and RNAs. Therefore, secretome-derivatives might offer huge advantages over cells through their synthesis and storage for long-term use. When considering the therapeutic significance and future prospects of ASCs, this review summarizes the recent developments made in harvesting, isolation, and characterization. Furthermore, this article also provides a deeper insight into secretome of ASCs mediating regenerative efficacy.

Section: Characterization Of Ascsmentioning

confidence: 99%

Revisiting the Advances in Isolation, Characterization and Secretome of Adipose-Derived Stromal/Stem Cells

Mishra²,

et al. 2018

IJMS

“…Recently, several experiments have been performed to demonstrate the existence of adipose tissue-derived progenitors that give rise to bone (Liu et al, 2007;Arrigoni et al, 2009;Song et al, 2011;Çapkın, 2012). It was reported in these studies that various bioactive factors, including dexamethasone, ascorbic acid, and β-glycerophosphate, induced osteogenic differentiation in both human and rat ADMSCs.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the osteogenic differentiation capacity of adipose tissuederived mesenchymal stem cells from humans and rats

Tayhan¹,

Taşdemir²,

Gürhan³

et al. 2016

Turk J Biol

IntroductionMany people of all ages all over the world suffer from serious bone diseases such as bone fractures, bone tumors, and osteoporosis. The limited treatment options for these disorders indicate a need for new therapies. Cellular therapy is one of the promising methods in orthopedic clinical trials (Pytlik et al., 2009;Seong et al., 2010). To date, the most popular cells used in cellular therapies are mesenchymal stem cells (MSCs). They can be isolated from different adult tissues such as the bone marrow, placenta, liver, adipose, periosteum, and testes (Vater et al., 2011). Adipose tissue is one of the most preferred sources because it can be obtained by less invasive methods and in larger quantities than the other adult tissues (Liu et al., 2007). Adipose tissue-derived MSCs (ADMSCs) have a great plasticity for variable cell types. For the characterization of ADMSC plasticity, one of the most widely accepted methods is differentiating them into osteoblasts, chondrocytes, and adipocytes in vitro (Vater et al, 2011).Recently, several experiments have been performed to demonstrate the existence of adipose tissue-derived progenitors that give rise to bone (Liu et al., 2007;Arrigoni et al., 2009;Song et al., 2011;Çapkın, 2012). It was reported Materials and methodsAll cell culture materials and differentiation reagents were purchased from Greiner Bio-One (Germany) and Sigma Chemical Co. (USA) respectively, unless otherwise noted. Alpha minimum essential medium (MEM) (HyClone, SH30007.04), Dulbecco's modified Eagle's medium (DMEM) (high glucose) (FG0435), fetal bovine serum (FBS) (S0115), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (L1613), gentamicin (A2710), phosphate buffered saline (PBS) (L1835), Hank's balanced salt solution Abstract: Mesenchymal stem cells (MSCs) can be found in many types of adult tissues such as the bone marrow, adipose, placenta, liver, and periosteum. Recently, adipose tissue-derived MSCs (ADMSCs) have become one the most preferred MSC types because of their fast proliferation rate, abundance, and high plasticity for variable cell types. It is known that ADMSCs are able to differentiate into various cells, including osteoblasts, so they are quite promising for orthopedic clinical trials. The present study aimed to compare the osteogenic differentiation conditions of MSCs from human adipose tissue (hADMSC) and those of MSCs from rat adipose tissue (rADMSC). Therefore, differentiation experiments with five different media and two (human and rat) ADMSC types were performed and the mineralization responses of hADMSCs and rADMSCs were different.

“…Previous studies have shown that vitamin D is dose dependent in serum [17,21] but it should also be noted that vitamin D also has a bone resorbing effect, especially with high therapeutic doses [17]. Thus, the selection of the optimal concentration must be determined based on further evidence.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, Masuyama et al [16] showed that 1.25-(OH) 2 D 3 could regulate collagen modification and maturation in an osteoblastic cell culture, which has been proven to be important in early bone formation [19]. Numerous studies have also suggested that these intriguing osteogenic influences of 1.25-(OH) 2 D 3 are dose dependent [17,20,21]. …”

Section: Introductionmentioning

confidence: 99%

The Influence of 1α.25-Dihydroxyvitamin D3 Coating on Implant Osseointegration in the Rabbit Tibia

Naito¹,

Jimbo²,

Vandeweghe³

et al. 2014

JOMR

ObjectivesThis study aims to evaluate bone response to an implant surface modified by 1α,25-dihydroxyvitamin D3 [1.25-(OH)2D3] in vivo and the potential link between 1.25-(OH) 2D3 surface concentration and bone response.Material and MethodsTwenty-eight implants were divided into 4 groups (1 uncoated control, 3 groups coated with 1.25-(OH)2D3 in concentrations of 10-8, 10-7 and 10-6 M respectively), placed in the rabbit tibia for 6 weeks. Topographical analyses were carried out on coated and uncoated discs using interferometer and atomic-force-microscope (AFM). Twenty-eight implants were histologically observed (bone-to-implant-contact [BIC] and new-bone-area [NBA]).ResultsThe results showed that the 1.25-(OH)2D3 coated implants presented a tendency to osseointegrate better than the non-coated surfaces, the differences were not significant (P > 0.05).ConclusionsThe effect of 1.25-(OH)2D3 coating to implants suggested possible dose dependent effects, however no statistical differences could be found. It is thought that the base substrate topography (turned) could not sustain sufficient amount of 1.25-(OH)2D3 enough to present significant biologic responses. Thus, development a base substrate that can sustain 1.25-(OH)2D3 for a long period is necessary in future studies.