Effects of Blood Contamination on Microtensile Bond Strength to Dentin of Three Self-etch Adhesives

Abstract

This study evaluated the effects of blood contamination and decontamination methods during different steps of bonding procedures on the microtensile bond strength of two-step self-etch adhesives to dentin. Sixty extracted human molars were ground flat to expose occlusal dentin. The 60 molars were randomly assigned to three groups, each treated with a different two-step self-etch adhesive: Clearfil SE Bond, AdheSE and Tyrian SPE. In turn, these groups were subdivided into five subgroups (n = 20), each treated using different experimental conditions as follows: control group-no contamination; contamination group 1-CG1: primer application/ contamination/primer re-application; contamination group 2-CG2: primer application/contamination/wash/dry/primer re-application; contamination group 3-CG3: primer application/adhesive application/light curing/contamination/ adhesive re-application/light curing; contamina- tion group 4-CG4: primer application/adhesive application/light curing/contamination/wash/ dry/adhesive re-application/light curing. Composite buildup was performed using Z250. After 24 hours of storage in distilled water at 37 degrees C, the bonded specimens were trimmed to an hourglass shape and serially sectioned into slabs with 0.6 mm2 cross-sectional areas. Microtensile bond strengths (MTBS) were assessed for each specimen using a universal testing machine. The data were analyzed by two-way ANOVA followed by a post hoc LSD test. SEM evaluations of the fracture modes were also performed. The contaminated specimens showed lower bond strengths than specimens in the control group (p < 0.05), with the exception of CG1 in the Clearfil SE group and CG2 and CG3 in the Tyrian SPE group. Among the three self-etch adhesives, the Tyrian SPE group exhibited a significantly lower average MTBS compared to the Clearfil SE Bond and AdheSE (p < 0.05) groups. Based on the results of the current study, it was found that blood contamination reduced the MTBS of all three self-etch adhesives to dentin, and water-rinsing was unable to overcome the effects of blood contamination.

Influence of Ankaferd Blood Stopper on shear bond strength of bonding systems

“…Bond strength of bonding systems to dentin is negatively influenced by blood contamination 10,29,30) . The manufacturer produced many haemostatic agents to stop the bleeding.…”

Effect of saliva and blood contamination on the bond strength of self-etching adhesive system- An in vitro study

“…[1–3] Any contamination of the prepared surface by saliva, blood or gingival crevicular fluid should be avoided in order to achieve a successful and durable bond between the resin composite and the tooth. [45] Achieving good moisture control is a common problem encountered in restorative dentistry, especially when rubber dam isolation is not feasible. [26] Many carious lesions are found in areas where it is difficult to obtain appropriate isolation for the use of dentin bonding agents, especially when the site is near or at the gingival margin.…”

“…[47] Several recent studies suggested that self-etch adhesives may be more resistant to salivary contamination because hydrophilic adhesive solutions, specifically products with acetone or ethanol based, may displace or diffuse through the salivary film to reach the underlying hydroxyapatite or collagen. [248] However, the effect of blood contamination on the adhesive properties of 1-step self-etching adhesives has not been fully understood.…”

“…[47] Several recent studies suggested that self-etch adhesives may be more resistant to salivary contamination because hydrophilic adhesive solutions, specifically products with acetone or ethanol based, may displace or diffuse through the salivary film to reach the underlying hydroxyapatite or collagen. [248] However, the effect of blood contamination on the adhesive properties of 1-step self-etching adhesives has not been fully understood. [24910] The aims of this present in vitro study were to determine the effect of saliva and blood contamination on shear bond strength of self-etching adhesive to enamel and dentin, and to compare the difference in bond strengths due to contamination before and after application of self-etch adhesive.…”

Avaliação clínica retrospectiva de restaurações cervicais de resina composta

“…Entretanto, como já mencionado, restaurar essas lesões com resina composta apresenta uma série de dificuldades técnicas que podem afetar os resultados clínicos. Dificuldades no isolamento, na adesão com a margem da dentina e o estresse de contração de polimerização levam a que realizar esses trabalhos seja considerado um procedimento extremamente sensível [26][27] . O cimento de ionômero de vidro tem sido bem usado devido à sua adesão química com a estrutura dentária e liberação de flúor [28][29] , além de ser uma técnica relativamente simples comparada à da resina.…”