Effects of blended microbial feed additives on performance, meat quality, gut microbiota and metabolism of broilers

Abstract

The present study investigated the effects of blend microbial feed additive (BMFA) in diet on performance, meat quality, gut microbiota and metabolism of broilers. In this study 240 seventy-day-old female Wenchang broilers were randomly allocated into four groups with five replicates of 12 broilers each. Broilers in the control group was fed only basal diet (S0), and the other three groups were fed the same basal diet supplemented with 0.2% (S1), 0.4% (S2), or 0.6% (S3) of BMFA, respectively. The trial continued for 54 days. The results showed that broilers in S2 and S3 had lower average daily feed intake (ADFI) compared with S0 and S1 (P < 0.05). However, diet supplementation with BMFA had no significantly influence on the average daily gain (ADG) and the ratio of ADFI to ADG (F/G) (P > 0.05). The highest thigh muscle percentage was observed in S2 (P < 0.05) among all groups. Diet supplementation with BMFA reduced the shear force in both breast and thigh muscles (P < 0.05) of broilers. An increase (P < 0.05) in the total unsaturated fatty acid (USFA), monounsaturated fatty acids (MUFA), and ratio of unsaturated fatty acids to saturated fatty acid (USFA/SFA) in breast muscles was observed in S3 compared with S0. It was found that the S3 had a relatively higher abundance of Lactobacillus (P < 0.001), as well as a lower abundance of the Bacteroides, Rikenellaceae RC9 gut group, Olsenella, Prevotellaceae UCG-001 and Prevotella (P < 0.05) than the S0. Correlation analysis indicated that a total of 17 differential metabolites between the S3 and S0 were significantly correlated with the 7 differential genera microflora. Overall, diet supplementation with 0.6% of BMFA can significantly improve the meat quality of broilers by decreasing the concentration of SFA and enhancing the levels of the total USFA, MUFA and USFA/SFA in breast muscles. Those findings were tightly bound to the higher proportion of Lactobacillus genus in the intestinal tract of broilers influenced by BMFA.

Dietary novel alkaline protease from Bacillus licheniformis improves broiler meat nutritional value and modulates intestinal microbiota and metabolites

“…The gut microbiota and its metabolites are intimately linked to the quality of broiler meat and the profiles of amino acids and fatty acids within it [ 51 – 54 ]. However, the effects of the novel protease on gut microbiota and metabolites and the potential correlation between differential microbiota, amino acids, and fatty acids in meat remain unclear.…”

Prickly ash seeds can promote healthy production of sheep by regulating the rumen microbial community

“…Bacteroidetes and Firmicutes are often the “core microbiome” of rumen in ruminants, and this study was no exception in substantiating the abovementioned finding (Weimer, 2015 ). These two dominant phyla work together to promote the degradation of fibrous and non-fibrous materials in the rumen to produce acetic acid and propionic acid for absorption and utilization by the body (Ye et al, 2022 ; Zhou et al, 2022b ). As a dominant phylum in the rumen, the main role of Bacteroidota is to promote the degradation of complex organic matter in ruminants, such as the degradation of carbohydrates, which can help the host to absorb and utilize polysaccharides efficiently (An et al, 2023 ; Wang et al, 2023c ).…”

Effects of mixed extract from two tropical plants on gut microbiome and metabolome in piglets

“…16S rRNA gene sequencing analysis of was accomplished using previously published approach (Deng et al, 2023; Zhou et al, 2022). After total bacterial community DNA was extracted and their quality and quantity were detected and estimated, the V3–V4 region of 16S rRNA was amplified used specific primers.…”