Effects of blastomere biopsy on post-natal growth and behavior in mice

Sampino, Silvestre; Zacchini, Federica; Świergiel, Artur H.; Modlinski, A.J.; Loi, Pasqualino; Ptak, Grazyna

doi:10.1093/humrep/deu145

Cited by 36 publications

(34 citation statements)

References 38 publications

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“…body weight, blood parameters or gross morphology of major internal organs) (BB: Cui et al 1993Cui et al , 1994TB: Gentry & Critser 1995). However, in the last decade, different research groups have described increased body weight/size and impaired behavior in BB mice and have suggested reduced fertility in adult females (Yu et al 2009, Zeng et al 2013, Sampino et al 2014, Wu et al 2014). …”

Section: General Overviewmentioning

confidence: 99%

“…Studies to assess epigenetic, behavioral, reproductive and/or metabolic health in the pre-or postnatal life following TB have not been published so far, while few research groups have performed studies to assess BB risk(s) and safety (Yu et al 2009, 2013, Sugawara et al 2012, Yang et al 2012, Zeng et al 2013, Sampino et al 2014, Sato et al 2014, Wu et al 2014. Thus, in the following sections, we will focus our attention on BB, rather than TB.…”

Section: General Overviewmentioning

confidence: 99%

“…No effect of BB was described with regard to pregnancy rate and litter size when comparing BB mice vs control(s) at reproductive age (Sampino et al 2014. However, Yu and coworkers showed that aged (i.e.…”

Section: Reproductive Fitness In Mice Conceived Following Blastomere mentioning

confidence: 99%

“…In the last decade, a few research groups have investigated whether BB could be associated with an increased risk of neurodevelopmental disorders (Yu et al 2009, Zeng et al 2013, Sampino et al 2014, Wu et al 2014. Behavioral tests showed that mice born following BB had impaired memory and motor coordination, increased anxiety and altered responses to threatening or stress stimuli.…”

Section: Neurodevelopmental Disorders Induced By Blastomere Biopsymentioning

confidence: 99%

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Embryo biopsy and development: the known and the unknown

Zacchini¹,

Arena²,

Abramik³

et al. 2017

Reproduction

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Preimplantation genetic diagnosis (PGD) has been introduced in clinical practice as a tool for selecting 'healthy' embryos before their transfer in utero. PGD protocols include biopsy of cleaving embryos (blastomere biopsy (BB)) or blastocysts (trophectoderm biopsy (TB)), followed by genetic analysis to select 'healthy' embryos for transfer in utero. Currently, TB is replacing the use of BB in the clinical practice. However, based on the European Society of Human Reproduction and Embryology Preimplantation Genetic Diagnosis Consortium reports, BB has been used in >87% of PGD cycles for more than 10 years. An exhaustive evaluation of embryo biopsy (both BB and TB) risks and safety is still missing. The few epidemiological studies available are quite controversial and/or are limited to normalcy at birth or early childhood. On the other hand, studies on animals have shown that BB can be a risk factor for impaired development, during both pre-and postnatal life, while little is known on TB. Thus, there is an urgent need of focused researches on BB, as it has contributed to give birth to children for more than 10 years, and on TB, as its application is significantly growing in clinical practice. In this context, the aim of this review is to provide a complete overview of the current knowledge on the short-, medium-and long-term effects of embryo biopsy in the mouse model. Reproduction (2017) 154 R143-R148

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Section: General Overviewmentioning

confidence: 99%

Section: General Overviewmentioning

confidence: 99%

Section: Reproductive Fitness In Mice Conceived Following Blastomere mentioning

confidence: 99%

Section: Neurodevelopmental Disorders Induced By Blastomere Biopsymentioning

confidence: 99%

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Embryo biopsy and development: the known and the unknown

Zacchini¹,

Arena²,

Abramik³

et al. 2017

Reproduction

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“…In some cases the embryo is frozen at -180° C and subsequently eliminated. In relation to the safety of the technique, effects on the growth and behavior of animals born after PGD have been shown (40). Nevertheless, such effects on humans have not been observed in preliminary studies (22,(41)(42)(43).…”

mentioning

confidence: 99%

Ethical Problems with the Preimplantation Genetic Diagnosis of Human Embryos

Marambio

Alcantara

2018

Acta bioeth.

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Abstract:The purpose of preimplantation genetic diagnosis by embryonary biopsy is to identify genetic alterations prior to the implantation of embryos produced by in vitro fertilization. The most important aim is the selection of genetically healthy embryos due to their genetic indemnity, but it can also be used to select the sex or, eventually, other detectable traits accrding to the wishes of the parents. This procedure has been the subject of scientific debates, in relation to the harm that it can cause to healthy embryos that are going to be implanted, and in relation to the interpretation of the genetic tests made. Ethical debates have also focused on the production of and respect for the life and the integrity of developing human beings. In this work, it is argued that most of the uses of PGD are morally reprehensible, because they are done with disregard to the dignity that should be granted to embryos as human persons.Key words: preimplantation genetic diagnosis, human embryos, ethical status of embryos, persons, dignity Problemas éticos con el diagnóstico genético preimplantacional de embriones humanosResumen: El diagnóstico genético preimplantacional (DGP) mediante biopsia embrionaria tiene como objeto la detección de alteraciones genéticas previamente a la implantación de embriones producidos por fertilización in vitro (FIV). Su finalidad más significativa es la selección de embriones por su indemnidad genética. También se puede emplear para seleccionar el sexo o eventualmente otras características detectadas según el deseo de los padres. Este procedimiento ha sido objeto de debates en el ámbito científico, por el eventual daño que puede ocasionar la técnica en embriones sanos que serán implantados y por las interpretaciones de los exámenes genéticos realizados. También ha sido objeto de debates en el ámbito ético-antropológico, en cuanto a la producción y al respeto a la vida e integridad de los seres humanos en desarrollo. En este trabajo se argumenta que los usos que se hacen del DGP son, en su gran mayoría, moralmente reprochables, por hacerse con desprecio de la dignidad que debe darse al embrión como persona humana. Palabras clave: diagnóstico genético preimplantacional, embriones humanos, estatus ético de los embriones, personas, dignidad Problemas éticos com diagnóstico pré-implantacional de embriões humanosResumo: O Diagnóstico genético pré-implantacional (PGD) por meio de biópsia embrionária visa a identificação de alterações genéticas prévias à implantação de embriões produzidos por fertilização in vitro (FIV). Seu propósito mais significativo é a seleção de embriões por sua característica genética. Ele também pode ser usado para selecionar o sexo ou, eventualmente, outras características identificadas de acordo com os desejos dos pais. Este procedimento tem sido tema de debate em âmbito científico, por eventual dano que pode ocaciosionar a técnica em embriões saudáveis que serão implantados e pela interpretações dos exames genéricos realizados. Ele também tem sido objeto de debate na área ...

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A potential high risk for fatty liver disease was found in mice generated after assisted reproductive techniques

Zhang

Zheng

et al. 2017

J of Cellular Biochemistry

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Abnormal gametogenesis and embryonic development may lead to poor health status of the offspring. The operations involved in the assisted reproductive technologies (ARTs) occur during the key stage of gametogenesis and early embryonic development. To assess the potential risk of abnormal lipid metabolism in the liver of adult ARTs offspring, two ARTs mice models derived from preimplantation genetic diagnosis (PGD group) and in vitro cultured embryos without biopsy (IVEM group) were constructed. And control mice were from in vivo naturally conceived (Normal group). The results showed that ARTs offspring had increased body weight and body fat content comparing to normal group. An increasing volume and amount of lipid droplets as well as lipid droplet fusion were found in the hepatocytes of ARTs mice, and a significantly increased liver TG content was also shown in the ARTs mice, which due to the increased TG synthesis and decreased TG transport in the liver. All the results indicated that the manipulations involved in ARTs might play an important role in the lipid accumulation of adult offspring. By analyzing the DNA methylation profiles of 7.5dpc embryos, we proposed that methylation deregulation of the genes related to liver development in ARTs embryos might contribute to the abnormal phenotype in the offspring. The study demonstrated that ARTs procedures have adverse effect on liver development which resulted in abnormal lipid metabolism and induced the potential high risk of fatty liver in adulthood.

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Effects of blastomere biopsy on post-natal growth and behavior in mice

Cited by 36 publications

References 38 publications

Embryo biopsy and development: the known and the unknown

Embryo biopsy and development: the known and the unknown

Ethical Problems with the Preimplantation Genetic Diagnosis of Human Embryos

A potential high risk for fatty liver disease was found in mice generated after assisted reproductive techniques

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