Effects of bisphenol A treatment during pregnancy on kidney development in mice: a stereological and histopathological study

Abstract

Bisphenol A (BPA) is a chemical found in plastics that resembles oestrogen in organisms. Developmental exposure to endocrine-disrupting chemicals, such as BPA, increases the susceptibility to type 2 diabetes (T2DM) and cardiovascular diseases. Animal studies have reported a nephron deficit in offspring exposed to maternal diabetes. The aim of this study was to investigate the prenatal BPA exposure effects on nephrogenesis in a mouse model that was predisposed to T2DM. This study quantitatively evaluated the renal structural changes using stereology and histomorphometry methods. The OF1 pregnant mice were treated with a vehicle or BPA (10 or 100 μg/kg/day) during days 9-16 of gestation (early nephrogenesis). The 30-day-old offspring were sacrificed, and tissue samples were collected and prepared for histopathological and stereology studies. Glomerular abnormalities and reduced glomerular formation were observed in the BPA offspring. The kidneys of the BPA10 and BPA100 female offspring had a significantly lower glomerular number and density than those of the CONTROL female offspring. The glomerular histomorphometry revealed a significant difference between the female and male CONTROL offspring for the analysed glomerular parameters that disappeared in the BPA10 and BPA100 offspring. In addition, the kidney histopathological examination showed typical male cuboidal epithelial cells of the Bowman capsule in the female BPA offspring. Exposure to environmentally relevant doses of BPA during embryonic development altered nephrogenesis. These structural changes could be associated with an increased risk of developing cardiometabolic diseases later in life.

Cat Kidney Glomeruli and Tubules Evaluated by Design‐Based Stereology

“…Therefore, total glomerular volume (the multiple of glomerular number and mean volume) is proposed as a useful indicator of filtration surface area (Nyengaard and Bendtsen, ). It is important to note that the glomerular number and the glomerular volume have an inverse relationship within the same species (Nuñez et al, ). Physiologically, reduction in nephron number may cause glomerular hyperfiltration, which leads to increase in glomerular hydrostatic pressure and glomerular hypertrophy to maintain renal function (Puelles et al, ).…”

“…Most stereological investigations on the kidney have been carried out on laboratory animals such as mouse (Arsenault et al, ; Walton et al, ; Nuñez et al, ) and rat (Şimşek et al, ; Khoshvakhti et al, ; Melchioretto et al, ; Seravalli et al, ), and also on other mammals such as nonhuman primates (Gubhaju and Black, ; Batchelder et al, ). However, apart from investigations on the kidney in pig (Cibulskyte et al, ; Karstoft et al, ; Lødrup et al, ,b), sheep (Bains et al, ; Wintour et al, ) and horse (Bolat et al, ), the morphometric features of kidney of other domestic animals have not been thoroughly investigated using stereology.…”

A Stereological Study of the Toxic Effects of Cerium Oxide during Pregnancy on Kidney Tissues in Neonatal NMRI Mice

“…Previous findings have indicated that CeO 2 passes through the placenta [34]. In mice, the development of the metanephros is considered to begin at E10.5-11 and ends at 7-10 days after birth [49,50]. The kidney, like all other major organs of the body, is susceptible to exposure to a Regulated differentiation and proliferation of mesenchymal cells and urinary epidermal primordial cells cause nephrogenesis in the embryonic period [50].…”

“…The kidney, like all other major organs of the body, is susceptible to exposure to a wide range of chemicals during developmental periods. Regulated differentiation and proliferation of mesenchymal cells and urinary epidermal primordial cells cause nephrogenesis in the embryonic period [ 50 ]. Kidney development in mice is completed two weeks after birth [ 51 ].…”

Cell proliferation in kidney carcinoma is inhibited by lncRNA GASL1

“…Therefore, we need an effective method for diagnosis of kidney cancer, which may lay the foundation for its potential treatment and cure. 5 There are already many signs of progress in the next sequencing technologies from the past years. Some reports have shown that only 10% of the genes were transcribed into coding RNAs, while 90% of the RNAs can be named as the noncoding RNAs (ncR-NAs).…”