Effects of bisphenol A on ovarian follicular development and female germline stem cells

Zhu, Xiaodong; Tian, Geng; B, Yu; Yang, Yanzhou; Wu, Ji

doi:10.1007/s00204-018-2167-2

Cited by 44 publications

(28 citation statements)

References 42 publications

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“…Many studies have provided convincing evidence that small molecular compounds affect the physiological functions of mammalian cells, such as proliferation, differentiation, autophagy, and apoptosis [13,14,29,45]. In this study, we found that C28 induced significant autophagy, and H3K27ac and ER stress might play roles in C28-induced autophagy of FGSCs in vitro.…”

Section: Discussionsupporting

confidence: 56%

C28-induced autophagy of female germline stem cells in vitro and its potential mechanisms

Chen¹,

Zhao²,

Tian³

et al. 2019

Preprint

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BackgroundThere are few studies indicating that small molecular compounds affect the proliferation, differentiation, apoptosis, and autophagy of female germline stem cells (FGSCs). However, the epigenetic regulatory mechanism of small molecular compounds that induce autophagy in FGSCs remains unknown.ResultsIn this study, we found that C28 reduced the viability and proliferation of FGSCs, respectively. Additionally, western blotting showed that the expression of autophagy marker light chain 3 beta II (LC3B-II) was significantly increased and expression of sequestosome-1 (SQSTM1) was significantly reduced in C28-treated groups. Immunofluorescence showed that, in C28-treated groups, the number of LC3B-II-positive puncta was increased significantly. These results indicated that C28 induced autophagy of FGSCs in vitro. ChIP-seq data showed that autophagy-related biological processes such as regulation of mitochondrial membrane potential, Golgi vesicle transport, and cellular response to reactive oxygen species were enriched. In addition, RNA-Seq showed that the expression of genes (Trib3, DDIT3, and ATF4) related to endoplasmic reticulum (ER) stress was enhanced by C28.ConclusionC28 could induce FGSC autophagy in vitro leading to a decrease in the number of FGSCs. H3K27ac and ER stress might play roles in C28-induced autophagy of FGSCs in vitro.

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Section: Discussionsupporting

confidence: 56%

C28-induced autophagy of female germline stem cells in vitro and its potential mechanisms

Chen¹,

Zhao²,

Tian³

et al. 2019

Preprint

Self Cite

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“…Nonetheless, against a backdrop of continued debate and scattered reports claiming from negative data to refute the existence of OSCs and the ability of adult mouse ovaries to generate new oocytes ([61,62,63,64,65]; see also [66,67,68,69]), studies of OSCs in rodent models continued to populate the scientific literature, with nearly 30 published primary studies now available reporting on the characteristic features and functional properties of these cells in the context of postnatal oogenesis in mice and rats [37,41,49,53,66,67,68,70,71,72,73,74,75,76,77,78,79,80,81,82,83,84,85,86,87,88,89,90,91,92]. These efforts have recently been paralleled by similar studies of OSCs in adult ovaries of cows [93,94], non-human primates [64,69] and pigs [95,96], underscoring the evolutionary conservation of the findings across diverse mammalian species.…”

Section: Germline Stem Cells In Adult Female Flies—so Why Not Femmentioning

confidence: 99%

Implications and Current Limitations of Oogenesis from Female Germline or Oogonial Stem Cells in Adult Mammalian Ovaries

Martin

Woods

Tilly

2019

Cells

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A now large body of evidence supports the existence of mitotically active germ cells in postnatal ovaries of diverse mammalian species, including humans. This opens the possibility that adult stem cells naturally committed to a germline fate could be leveraged for the production of female gametes outside of the body. The functional properties of these cells, referred to as female germline or oogonial stem cells (OSCs), in ovaries of women have recently been tested in various ways, including a very recent investigation of the differentiation capacity of human OSCs at a single cell level. The exciting insights gained from these experiments, coupled with other data derived from intraovarian transplantation and genetic tracing analyses in animal models that have established the capacity of OSCs to generate healthy eggs, embryos and offspring, should drive constructive discussions in this relatively new field to further exploring the value of these cells to the study, and potential management, of human female fertility. Here, we provide a brief history of the discovery and characterization of OSCs in mammals, as well as of the in-vivo significance of postnatal oogenesis to adult ovarian function. We then highlight several key observations made recently on the biology of OSCs, and integrate this information into a broader discussion of the potential value and limitations of these adult stem cells to achieving a greater understanding of human female gametogenesis in vivo and in vitro.

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“…As the number of chemicals classified as EDCs is increasing, EDCs are being linked with long-term health consequences. Animal models and human cohorts have associated increasing exposure to EDCs with decreased fertility, altered thyroid hormone levels, and increased risk of cancer in people directly exposed, as well as increased risk of adverse health outcomes in the children of those that were directly exposed [5][6][7][8][9][10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Exposure to polybrominated biphenyl (PBB) associates with genome-wide DNA methylation differences in peripheral blood

et al. 2019

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In 1973, Michigan residents were exposed to polybrominated biphenyl (PBB) when it was accidentally added to farm animal feed. Highly exposed individuals and their children have experienced endocrine-related health problems, though the underlying mechanism behind these remains unknown. We investigated whether PBB exposure is associated with variation in DNA methylation in peripheral blood samples from 658 participants of the Michigan PBB registry using the MethylationEPIC BeadChip, as well as investigated what the potential function of the affected regions are and whether these epigenetic marks are known to associate with endocrine system pathways. After multiple test correction (FDR <0.05), 1890 CpG sites associated with total PBB levels. These CpGs were not enriched in any particular biological pathway, but were enriched in enhancer and insulator regions, and depleted in regions near the transcription start site or in CpG islands (p < 0.05). They were also more likely to be in ARNT and ESR2 transcription factor binding sites (p = 3.27e-23 and p = 1.62e-6, respectively), and there was significant overlap between CpGs associated with PBB and CpGs associated with estrogen (p < 2.2e-16). PBBassociated CpGs were also enriched for CpGs known to be associated with gene expression in blood (eQTMs) (p < 0.05). These eQTMs were enriched for pathways related to immune function and endocrine-related autoimmune disease (FDR <0.05). These results indicate that exposure to PBB is associated with differences in epigenetic marks that suggest that it is acting similarly to estrogen and is associated with dysregulated immune system pathways. ARTICLE HISTORY

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Effects of bisphenol A on ovarian follicular development and female germline stem cells

Cited by 44 publications

References 42 publications

C28-induced autophagy of female germline stem cells in vitro and its potential mechanisms

C28-induced autophagy of female germline stem cells in vitro and its potential mechanisms

Implications and Current Limitations of Oogenesis from Female Germline or Oogonial Stem Cells in Adult Mammalian Ovaries

Exposure to polybrominated biphenyl (PBB) associates with genome-wide DNA methylation differences in peripheral blood

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