Effects of Bioremediation Agents on Oil Degradation in Mineral and Sandy Salt Marsh Sediments

Lin, Qinhao; Mendelssohn, I. A.; Henry, Charles B.; Roberts, Paulene O.; Walsh, Maud M.; Overton, Edward B.; Portier, Ralph J.

doi:10.1080/09593332008616878

Cited by 39 publications

(22 citation statements)

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“…Most of these studies have examined terrestrial plants and agricultural chemicals (1,2,27); few have looked at the influence of plant-associated microorganisms on the fate of PCBs (15,16) and PAHs (34,39). There have been a limited number of studies on PAH degradation involving wetland or salt marsh ecosystems, but none have studied the diversity of PAH-degrading microorganisms present (28,30,49).…”

Section: Discussionmentioning

confidence: 99%

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Isolation and Characterization of Polycyclic Aromatic Hydrocarbon-Degrading Bacteria Associated with the Rhizosphere of Salt Marsh Plants

Daane¹,

Harjono²,

Zylstra

et al. 2001

Appl Environ Microbiol

268

122

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Polycyclic aromatic hydrocarbon (PAH)-degrading bacteria were isolated from contaminated estuarine sediment and salt marsh rhizosphere by enrichment using either naphthalene, phenanthrene, or biphenyl as the sole source of carbon and energy. Pasteurization of samples prior to enrichment resulted in isolation of gram-positive, spore-forming bacteria. The isolates were characterized using a variety of phenotypic, morphologic, and molecular properties. Identification of the isolates based on their fatty acid profiles and partial 16S rRNA gene sequences assigned them to three main bacterial groups: gram-negative pseudomonads; grampositive, non-spore-forming nocardioforms; and the gram-positive, spore-forming group, Paenibacillus. Genomic digest patterns of all isolates were used to determine unique isolates, and representatives from each bacterial group were chosen for further investigation. Southern hybridization was performed using genes for PAH degradation from Pseudomonas putida NCIB 9816-4, Comamonas testosteroni GZ42, Sphingomonas yanoikuyae B1, and Mycobacterium sp. strain PY01. None of the isolates from the three groups showed homology to the B1 genes, only two nocardioform isolates showed homology to the PY01 genes, and only members of the pseudomonad group showed homology to the NCIB 9816-4 or GZ42 probes. The Paenibacillus isolates showed no homology to any of the tested gene probes, indicating the possibility of novel genes for PAH degradation. Pure culture substrate utilization experiments using several selected isolates from each of the three groups showed that the phenanthrene-enriched isolates are able to utilize a greater number of PAHs than are the naphthalene-enriched isolates. Inoculating two of the gram-positive isolates to a marine sediment slurry spiked with a mixture of PAHs (naphthalene, fluorene, phenanthrene, and pyrene) and biphenyl resulted in rapid transformation of pyrene, in addition to the two-and three-ringed PAHs and biphenyl. This study indicates that the rhizosphere of salt marsh plants contains a diverse population of PAH-degrading bacteria, and the use of plant-associated microorganisms has the potential for bioremediation of contaminated sediments.

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Section: Discussionmentioning

confidence: 99%

“…Wetlands have a higher biological activity than many other ecosystems, and they support enhanced biotransformation of toxic chemicals (25). Experiments with Spartina salt marsh mesocosms indicated that biodegradation of oil was influenced by flooding and fertilization conditions (28,49).…”

mentioning

confidence: 99%

Isolation and Characterization of Polycyclic Aromatic Hydrocarbon-Degrading Bacteria Associated with the Rhizosphere of Salt Marsh Plants

Daane¹,

Harjono²,

Zylstra

et al. 2001

Appl Environ Microbiol

268

122

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“…Some of the hydrocarbons such as PAHs, can be adsorbed to humic substances. The sorption characteristics of hydrocarbons to soil can further depend on the soil matrix; in a marsh environment with multiple soil types, a greater reduction of hydrocarbons was observed in sandy soils than in mineral soils (Lin et al 1999). …”

Section: Introductionmentioning

confidence: 99%

Characterizing Microbial Activity and Diversity of Hydrocarbon-Contaminated Sites

Ding¹,

Sun²,

Dou³

et al. 2013

Hydrocarbon

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“…Assessments of nutrient effects on oil degradation in situ have been confounded by the difficulty of randomly interspersing treatments and replicating adequately (Venosa et al 1996). Mesocosm studies, allowing for controlled manipulation of key variables, have found that nutrient addition generally stimulates oil breakdown but also interacts with other factors such as soil type (Lin et al 1999), plant growth (Lin and Mendelssohn 1998) and soil aeration (Dowty et al 2001). It is therefore important to explore the role that these other interacting factors together may have on the degradation of oil.…”

Section: Introductionmentioning

confidence: 99%

Marsh plants mediate the influence of nitrogen fertilization on degradation of oil from the Deepwater Horizon spill

et al. 2015

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Abstract. Coastal wetlands are commonly exposed to hydrocarbon pollutants derived from extraction disasters like the Deepwater Horizon oil spill. Naturally occurring microbes can degrade oil, but the rate of oil degradation depends heavily on the key chemical and biological factors. The goal of this study was to determine the influence of interactions between marsh plants and nitrogen (N) on the degradation of oil from the Deepwater Horizon oil spill. Oil disappearance was measured with gas chromatography (GC) focusing on the change in C 18 n-alkane-to-phytane ratio of oil, and instantaneous oil degradation rates were measured using an instantaneous carbon isotopic partitioning method. N addition often stimulates oil decomposition in soil slurries, but the effect of N in our mesocosms depended on plant species. N addition accelerated oil degradation in Spartina alterniflora mesocosms but slowed oil degradation in Spartina patens mesocosms. Across all plant and N treatments, oil degradation related to plant root growth. In many ecosystems including marshes, N addition has been shown to diminish root growth by reducing the need for nutrient foraging. Where N addition reduces root growth, N may ultimately exacerbate oxygen scarcity in marsh soils possibly negating or reversing the positive, direct effects that N has on oil degradation. Based on these findings, fertilization strategies that maximize marsh plant root growth will be the most effective at increasing the microbial degradation of oil and will have the greatest potential to mitigate the impacts of oil in marsh ecosystems.

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Effects of Bioremediation Agents on Oil Degradation in Mineral and Sandy Salt Marsh Sediments

Cited by 39 publications

References 0 publications

Isolation and Characterization of Polycyclic Aromatic Hydrocarbon-Degrading Bacteria Associated with the Rhizosphere of Salt Marsh Plants

Isolation and Characterization of Polycyclic Aromatic Hydrocarbon-Degrading Bacteria Associated with the Rhizosphere of Salt Marsh Plants

Characterizing Microbial Activity and Diversity of Hydrocarbon-Contaminated Sites

Marsh plants mediate the influence of nitrogen fertilization on degradation of oil from the Deepwater Horizon spill

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