Effects of Bioactive Pulp-capping Materials on Cell Viability, Differentiation, and Mineralization Behaviors of Human Dental Pulp Stem Cells <i>In Vitro</i>

Güngör, Ayça Sarıalioğlu; Durmuş, Ezgi; Kurt, Belma Zengin; Kocyigit, A; Dalkilic, EE; Arısu, Hacer Deniz

doi:10.2341/22-023-l

Cited by 4 publications

(4 citation statements)

References 49 publications

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“…Likewise, similar behavior showed the alginate microgel with low-CaP (Type M1) with high ALP activity in comparison with the control group at 14 days of culture. This level of ALP activity showed the modulation of the DPSCs cells under the presence of microgels composite as filler for the regeneration of the dentin-pulp complex, and these results are in agreement with recent experimental evidence about the promotive role of bioceramics on the DPSCs activities regarding osteogenic behavior [57][58][59][60]. In perspective, further investigations should be carried out to detect selected chemical targets involved in the osteogenic differentiation mechanisms of DPSCs, i.e., growth factors such as BMP-2 or signaling pathways such as Wnt/β-catenin, cytokines.…”

Section: Resultssupporting

confidence: 92%

Mineralized Microgels via Electrohydrodynamic Atomization: Optimization and In Vitro Model for Dentin–Pulp Complex

Cruz-Maya,

Altobelli,

Alvarez-Perez

et al. 2023

Gels

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There is growing interest in the use of micro-sized hydrogels, including bioactive signals, as efficient platforms for tissue regeneration because they are able to mimic cell niche structure and selected functionalities. Herein, it is proposed to optimize bioactive composite microgels via electrohydrodynamic atomization (EHDA) to regenerate the dentin–pulp complex. The addition of disodium phosphate (Na2HPO4) salts as mineral precursors triggered an in situ reaction with divalent ions in solution, thus promoting the encapsulation of different amounts of apatite-like phases. Morphological analysis via image analysis of optical images confirmed a narrow distribution of perfectly rounded particles, with an average diameter ranging from 223 ± 18 μm to 502 ± 64 μm as a function of mineral content and process parameters used. FTIR, TEM, and EDAX analyses confirmed the formation of calcium phosphates with a characteristic Ca/P ratio close to 1.67 and a needle-like crystal shape. In vitro studies—using dental pulp stem cells (DPSCs) in crown sections of natural teeth slices—showed an increase in cell viability until 14 days, recording a decay of proliferation at 21 days, independent on the mineral amount, suggesting that differentiation is started, as confirmed by the increase of ALP activity at 14 days. In this view, mineralized microgels could be successfully used to support in vitro osteogenesis, working as an interesting model to study dental tissue regeneration.

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Section: Resultssupporting

confidence: 92%

Mineralized Microgels via Electrohydrodynamic Atomization: Optimization and In Vitro Model for Dentin–Pulp Complex

Cruz-Maya,

Altobelli,

Alvarez-Perez

et al. 2023

Gels

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“…Güngör et al 56 in their in vitro studies, they evaluated the effect of bioactive pulp capping materials on human dental pulp stem cells (hDPSC) in terms of cell viability and bioactivity through mineralization potential. In their study, primary hDPSCs were cultured with experimental nBG, Biodentine, TheraCal LC, and ProRoot mineral trioxide aggregate (MTA) extracts.…”

Section: Cytotoxicity Studies On Resin-containing Materialsmentioning

confidence: 99%

An overview to biocompatibility of resin based restorative materials

Avcılar,

Bakır,

Bakır

2023

JDSE

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Nowadays, resin based materials find a wide range of use in dentistry due to their aesthetic properties, mechanical durability and cost advantages. Dentistry materials; They can have an effect because they are in direct contact with various tissues such as gums, tongue, lips and cheeks, in addition to periodontium, pulp, dentin and enamel. It is important that resin materials produced with new technologies to be used in restorative dentistry not only have mechanical, physical, functional and aesthetic properties, but also be carefully evaluated in terms of biological compatibility. The purpose of this review is to review the basic concepts and methods related to biocompatibility, to present data from studies on the cytotoxicity of resin-based materials, and finally to make recommendations for clinical applications.

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“…Nonetheless, it was also reported that an inflammatory microenvironment could alter the hallmarks of SCAPs, leading to an inhibitory or increasing effect on osteogenic differentiation ( 11 , 12 ). Effective osteogenic differentiation of MSCs is regulated by numerous factors, including physical, chemical, and biological factors, which may stimulate different signaling pathways, transcription factors, and microRNAs (miRNAs), to direct MSCs differentiated toward osteoblast lineage ( 13 – 16 ). However, the exact role of miRNAs in promoting osteogenic differentiation has yet to be fully understood.…”

Section: Introductionmentioning

confidence: 99%

MiR-199a-5P promotes osteogenic differentiation of human stem cells from apical papilla via targeting IFIT2 in apical periodontitis

Huang

Zheng

et al. 2023

Front. Immunol.

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IntroductionPeriapical alveolar bone loss is the common consequence of apical periodontitis (AP) caused by persistent local inflammation around the apical area. Human stem cells from apical papilla (hSCAPs) play a crucial role in the restoration of bone lesions during AP. Studies have recently identified the critical role of microRNAs (miRNAs) involved in AP pathogenesis, but little is known about their function and potential molecular mechanism, especially in the osteogenesis of hSCAPs during AP. Here, we investigated the role of clinical sample-based specific miRNAs in the osteogenesis of hSCAPs.MethodsDifferential expression of miRNAs were detected in the periapical tissues of normal and patients with AP via transcriptomic analysis, and the expression of miR-199a-5p was confirmed by qRT-PCR. Treatment of hSCAPs with miR-199a-5p mimics while loaded onto beta-tricalcium phosphate (β-TCP) ceramic particle scaffold to explore its effect on osteogenesis in vivo. RNA binding protein immunoprecipitation (RIP) and Luciferase reporter assay were conducted to identify the target gene of miR-199a-5p.ResultsThe expression of miR-199a-5p was decreased in the periapical tissues of AP patients, and miR-199a-5p mimics markedly enhanced cell proliferation and osteogenic differentiation of hSCAPs, while miR-199a-5p antagomir dramatically attenuated hSCAPs osteogenesis. Moreover, we identified and confirmed Interferon Induced Protein with Tetratricopeptide Repeats 2 (IFIT2) as a specific target of miR-199a-5p, and silencing endogenous IFIT2 expression alleviated the inhibitory effect of miR-199a-5p antagomir on the osteogenic differentiation of hSCAPs. Furthermore, miR-199a-5p mimics transfected hSCAPs loaded onto beta-tricalcium phosphate (β-TCP) scaffolds induced robust subcutaneous ectopic bone formation in vivo.DiscussionThese results strengthen our understanding of predictors and facilitators of the key AP miRNAs (miR-199a-5p) in bone lesion repair under periapical inflammatory conditions. And the regulatory networks will be instrumental in exploring the underlying mechanisms of AP and lay the foundation for future regenerative medicine based on dental mesenchymal stem cells.

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Effects of Bioactive Pulp-capping Materials on Cell Viability, Differentiation, and Mineralization Behaviors of Human Dental Pulp Stem Cells In Vitro

Cited by 4 publications

References 49 publications

Mineralized Microgels via Electrohydrodynamic Atomization: Optimization and In Vitro Model for Dentin–Pulp Complex

Mineralized Microgels via Electrohydrodynamic Atomization: Optimization and In Vitro Model for Dentin–Pulp Complex

An overview to biocompatibility of resin based restorative materials

MiR-199a-5P promotes osteogenic differentiation of human stem cells from apical papilla via targeting IFIT2 in apical periodontitis

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