Effects of bio-mulching on wheat soil microbial community and carbon utilization efficiency in southwest China

Liu, Bangyan; Cheng, Xin; He, Xian; Bei, Qicheng; Dai, Yisha; Wang, Yifan; Zhu, Bo; Zhang, Kangping; Tian, Xiaoqin; Duan, Meichun; Xie, Xun; Wang, Longchang

doi:10.1016/j.catena.2022.106260

Cited by 10 publications

(14 citation statements)

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“…This study was performed on the experiment farm of Southwest University in Chongqing, China (29°81′N, 106°41′E; elevation 244 m). The soil type was classi ed as purple soil (dystric regosols) on dry land (Liu et al, 2022). The soil had a pH of 6.0, a moisture content of 23.73%, an organic matter content of 18.62 g•kg − 1 , total nitrogen content of 1.07 g•kg − 1 , and available nitrogen content of 99.35 mg•kg − 1 before the investigation.…”

Section: The Experiments Design and Samples Collectionmentioning

confidence: 99%

“…The extracted DNA quality was measured by a NanoDrop 2000 UV-vis spectrophotometer (Thermo Scienti c, Wilmington, USA) (OD260/280 = 1.86 ~ 1.93, and the total DNA concentration range was 29.3 ~ 54.0 ng•ul − 1 ). A thermocycler PCR system (GeneAmp® 9700, ABI, USA) was used to amplify the hypervariable regions of bacteria (V3-V4, primers were 338F and 806R) and fungi (ITS1, primers were ITS1F and ITS2R) (Adams et al, 2013;Xu et al, 2016;Liu et al, 2022). The PCR (ABI GeneAmp® type 9700) ampli cation conditions were as follows: pre-denaturation at 95°C for 3 minutes, denaturation at 95°C for 30 seconds, annealing at 55°C for 30 seconds, and elongation at 72°C for 45 seconds as a cycle (bacteria and fungi performed 28 cycles and 36 cycles, respectively), extension 10 minutes at 72°C.…”

Section: Dna Extraction Pcr Ampli Cation Sequencing and Data Processingmentioning

confidence: 99%

“…The α-diversity of the microbial community was calculated through the Tukey test of ANOVA analysis to compare the differences within samples, including the indexes of Chao 1, Shannon, Simpson, and others. Based on the distance matrix of Bray-Curtis, the analysis of Adonis and NMDS (non-metric multidimensional scaling) were generated by the 'vegan' package to evaluate the variation in microbial community structure (Oksanen et al, 2016;Liu et al, 2022). The microbial relative abundance was calculated based on the OTUs tables, and the 'pheatmap' package was used to build the heatmap of microbial community relative abundance and carbon sources metabolism (Kolde, 2018).…”

Section: Date Analysismentioning

confidence: 99%

“…The bubble chart of soil factors was created by 'ggpubr' packages. The heatmap between bacterial biogeochemical functions and family lever communities was generated by R packages 'reshape', 'psych', 'ggtree', 'dplyr', 'tidyverse', and 'aplot' (Yu et al, 2018;Revelle, 2022;Liu et al 2022). The Mantel test was used to investigate the relationship between phylum-level microbial communities and soil carbon and nitrogen fractions, soil organic acid content, and soil enzyme activity in the presence of straw mulch.…”

Section: Date Analysismentioning

confidence: 99%

“…Thereby, this research was conducted by using wheat straw mulch patterns with varying amounts (0 kg/ha, 2250 kg/ha, and 4500 kg/ha), applying the High-throughput sequencing technology to classify the communities of bacteria and fungi and predict their functions, respectively. Additionally, the methods provided in our earlier study were used to assess the rates of carbon metabolism in microbial communities (Liu et al, 2022). Simultaneously, the measurements of soil organic acids, enzyme activity, and C and N fractions were made using HPLC (High-Performance Liquid Chromatography), classic enzymology methods, and a TOC analyzer, among others.…”

Section: Introductionmentioning

confidence: 99%

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Straw mulch improves soil carbon and nitrogen cycle by mediating soil microbial community structure in maize field

Liu¹,

Dai²,

Cheng³

et al. 2022

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Aims This study was conducted to investigate the capability of the microbial community networks and soil variables to promote carbon and nitrogen cycles in maize fields under straw mulch. Methods We covered the surface soil of the maize field with different amounts of wheat straw (0 kg/ha, 2250 kg/ha, and 4500 kg/ha), then used High-throughput sequencing, Biology ECO-plate, traditional enzymology, TOC analyzer, and HPLC to measure soil microbial community composition and functions, characteristics of microbial carbon source metabolism, carbon and nitrogen fraction, enzyme activity, and organic acid content in maize rhizosphere and non-rhizosphere. Results Our studies indicated that short-term straw mulch significantly influenced microbial β-diversity. The function predicts results revealed that straw mulch significantly increased the relative abundances of bacteria belonging to chemoheterotrophy, aerobic chemoheterotrophy, ureolysis, and nitrogen fixation while inhibiting fermentation and nitrate reduction in maize rhizosphere soil. The Biology ECO-plate results illustrated that straw mulch weakened the metabolism capacity of microbial labile carbon resources, resulting the soil labile C and N fractions raised. Straw mulch primarily regulated the community structure of Firmicutes, Ascomycota, and Basidiomycota. Those microbial communities direct effected on readily oxidizable organic carbon, microbial biomass carbon, available nitrogen, ammonium nitrogen, nitrate nitrogen, and dissolved organic carbon, and indirect effected on these C and N fractions by directly influencing malic acid content and activity of cellulase, protease, and amylase. Conclusions Our findings imply that straw mulch accelerate soil C and N cycle in maize field by mediating microbial community structuresto boost labile C and N components.

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Section: The Experiments Design and Samples Collectionmentioning

confidence: 99%

Section: Dna Extraction Pcr Ampli Cation Sequencing and Data Processingmentioning

confidence: 99%

Section: Date Analysismentioning

confidence: 99%

Section: Date Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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