1980
DOI: 10.1042/bj1880321
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Effects of bile salts on the plasma membranes of isolated rat hepatocytes

Abstract: The conjugated trihydroxy bile salts glycocholate and taurocholate removed approx. 20--30% of the plasma-membrane enzymes 5'-nucleotidase, alkaline phosphatase and alkaline phosphodiesterase I from isolated hepatocytes before the onset of lysis, as judged by release of the cytosolic enzyme lactate dehydrogenase. The conjugated dihydroxy bile salt glycodeoxycholate similarly removed 10--20% of the 5'-nucleotidase and alkaline phosphatase activities, but not alkaline phosphodiesterase activity; this bile salt ca… Show more

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Cited by 131 publications
(62 citation statements)
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“…In a normal liver, parts of the hepatocytic membrane are continuously solubilized and repaired by regeneration [138]. This process is altered in cholestasis.…”
Section: Systemmentioning
confidence: 99%
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“…In a normal liver, parts of the hepatocytic membrane are continuously solubilized and repaired by regeneration [138]. This process is altered in cholestasis.…”
Section: Systemmentioning
confidence: 99%
“…Membrane-damaging properties of bile acids in acute cholestasis or in liver disease accompanied by cholestasis are related to the detergent effect of bile salts [138]. Also im portant are the number and orientation of hydroxyl groups in the bile acid molecule and the presence of conjugation with taurine or glycine [149], A trihydroxy bile acid (cholate) is less damaging than the dihydroxy-or even the monohydroxy bile acid lithocholate.…”
Section: Systemmentioning
confidence: 99%
See 1 more Smart Citation
“…Recent data demonstrating that ursodeoxycholate (the 7-beta epimer of the primary bile salt chenodeoxycholate [CDC]') ameliorates human cholestatic liver disease have stimulated renewed interest in the cellular mechanisms of lethal bile salt injury (6-1 1 ). Because bile salts are detergents and form micelles, bile acid cytotoxicity has been attributed to their detergent properties (1,7,8). However, changes in cytosolic free calcium (Ca,2") and ATP have also been proposed as nondetergent mechanisms for bile salt-associated cytotoxicity.…”
Section: Introductionmentioning
confidence: 99%
“…All of these are found in mixed micelles with phosphatidyl choline and cholesterol. 65 The concentration of bile salts is approximately 20 mM. b5 The activity of alkaline phosphatase may vary from tens to thousands of units per litre.…”
Section: Alkaline Phosphatase In Cholestasis 407mentioning
confidence: 99%