Effects of bifidobacterial cytoplasm, cell wall and exopolysaccharide on mouse lymphocyte proliferation and cytokine production

Effects of bifidobacterial cytoplasm peptide and protein fractions on mouse lymphocyte proliferation and cytokine production

“…Iliev et al (2005) demonstrated that chromosomal DNA motifs from Lactobacillus rhamnosus GG are active in both murine and human immune cells. We obtained a similar pattern in our previous study (Amrouche et al 2006), which is consistent with results recently reported by Lee et al (2004), who mentioned that administration of fractions of Lactobacillus casei and Bifidobacterium longum cytoplasm to mice for four weeks enhanced the number of total T cells, NK cells and MHC class II'/ cells, and CD 4 ( CD 8 ' T cells. Bacterial peptides and proteins, notably enzymes, have been reported to influence both specific and non-specific immune responses (Dobrotina et al 1992, Houba et al 1992, Panaro & Mitolo 1999.…”

“…In our previous work (Amrouche et al 2006), we demonstrated that cell wall and cytoplasm from bifidobacteria stimulated in vitro both lymphocyte proliferation and cytokine production. In this study, we aimed to characterize peptide or protein fractions from Bifidobacterium lactis Bb12 cytoplasm that may be involved in immunomodulation.…”

Genomic Insights into Bifidobacteria

“…These inflammatory and immune defense responses are proposed to be triggered by recognition of commensal bifidobacteria via Toll-like receptors (TLRs) of the host innate immune system (242). Furthermore, cell wall constituents and unmethylated CpG DNA motifs of bifidobacteria are also believed to be involved in modulation of innate immune responses (8,166).…”

“…While this may be one function of EPS production by bifidobacteria, another appears to be as a fermentable carbohydrate source for other gut bacteria, including bifidobacteria (264). While some have speculated that bifidobacterial EPS may have immunomodulatory effects on the host, it was not found to stimulate lymphocyte proliferation or cytokine secretion, unlike cell wall extracts (8).…”

Effects of Bifidobacterium Strains Treated with Gastrointestinal Enzymes on Cytokine Induction in RAW 264.7 Macrophage Cells

“…It has been reported previously that these probiotic bacteria are capable of enhancing a variety of host immunological functions via activation of macrophages and lymphocytes (Hatcher and Lambrecht, 1993;Kirjavainen et al, 1999;Sekine et al, 1994), antibody synthesis (Fukushima et al, 1999; Link-Amster et al, 1994), T-and B-cell proliferation (Kang et al, 1994;Takahashi et al, 1993), and antitumor activity (Rafter, 1995;Sekine et al, 1995). Many prior studies have focused principally on the ability of viable cells, heat-killed cells, peptidoglycan, teichoic acid, and/or cell-free extract to induce several macrophage mediators, including nitric oxide, hydrogen peroxide, and cytokines in macrophages (Amrouche et al, 2006;Ouwehand et al, 1999). It has been reported previously that immune network systems are modulated through the variety of soluble molecules secreted by immune cells (Arai et al, 1990).…”