Effects of Betaine and Kaempferol On some criteria related to glucose homeostasis in Methionine Over Load Rats -part 2

Abstract

This study was designed to evaluate the role of Kaempferol(KPF) and Betaine (BET) in attenuating deleterious effects of methionine overload on some parameters related to diabetes in rats. Thirty (30) adult rats were randomly selected and divided equally into five experimental groups and treated for 45 days as the follows: G1:Rats in this group served as control, Rats in all other groups (G2,G3,G4 and G5 were received 100mg/kg B.W of D-L methionine in drinking water, and received orally as follows: Rats in group G3 were given 150 mg/kg b.w. of KPF, rats in group G4were given 250 mg/kg b.w. BET, rats in group G5 were given 150 mg/kg b.w. of KPF , 250 mg/kg b.w. BET. Fasting blood sample were collected by cardiac puncture technique at the end of the experiments and serum were collected for estimation glycemic indices,. C-peptide and B12 concentration. The results of the experiment that showed given D-L methionine for 45 days(G2group) caused disturbance in glycemic indices as detected by elevation in serum glucose and decrease in serum insulin hormone concentration) and decrease in serum C-peptide and B12 concentration. While oral intubation of KPF and BET or their combination caused correction of all recorded parameters.