Effects of benzophenone-3 on the green alga Chlamydomonas reinhardtii and the cyanobacterium Microcystis aeruginosa

Mao, Feijian; He, Yiliang; Kushmaro, Ariel; Gin, Karina Yew-Hoong

doi:10.1016/j.aquatox.2017.09.029

Cited by 66 publications

(40 citation statements)

References 46 publications

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“…The 7-day EC 50 value for BP3 was 143 µg/L. BP3 EC 50 values on several microalgae species have been reported previously to be roughly in the 100 µg/L to 20 mg/L range [17,21,41,42]. Seawater BP3 concentrations in the µg/L range have been frequently reported in the literature [5,10].…”

Section: Growth Rate and Ec 50 Valuesmentioning

confidence: 64%

“…Several studies have demonstrated that some of these compounds can disrupt survival [12][13][14]. behavior [15,16], growth [14,17,18], development [19,20], metabolism [21][22][23], gene expression [24,25] and reproduction [15,26,27] in various species. It should be noted that most toxicological studies on organic UV filters were conducted on BP3, EMC and 4-MBC [7].…”

Section: Introductionmentioning

confidence: 99%

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Effect of 10 UV Filters on the Brine Shrimp Artemia salina and the Marine Microalga Tetraselmis sp.

Thorel

Clergeaud

Jaugeon

et al. 2020

Toxics

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The presence of pharmaceutical and personal care product (PPCP) residues in the aquatic environment is an emerging issue due to their uncontrolled release through gray water, and accumulation in the environment that may affect living organisms, ecosystems and public health. The aim of this study is to assess the toxicity of benzophenone-3 (BP-3), bis-ethylhexyloxyphenol methoxyphenyl triazine (BEMT), butyl methoxydibenzoylmethane (BM), methylene bis-benzotriazolyl tetramethylbutylphenol (MBBT), 2-ethylhexyl salicylate (ES), diethylaminohydroxybenzoyl hexyl benzoate (DHHB), diethylhexyl butamido triazone (DBT), ethylhexyl triazone (ET), homosalate (HS) and octocrylene (OC) on marine organisms from two major trophic levels, including autotrophs (Tetraselmis sp.) and heterotrophs (Artemia salina). In general, results showed that both HS and OC were the most toxic UV filters for our tested species, followed by a significant effect of BM on Artemia salina due to BM—but only at high concentrations (1 mg/L). ES, BP3 and DHHB affected the metabolic activity of the microalgae at 100 µg/L. BEMT, DBT, ET, MBBT had no effect on the tested organisms, even at high concentrations (2 mg/L). OC toxicity represents a risk for those species, since concentrations used in this study are 15–90 times greater than those reported in occurrence studies for aquatic environments. For the first time in the literature, we report HS toxicity on a microalgae species at concentrations complementing those found in aquatic environments. These preliminary results could represent a risk in the future if concentrations of OC and HS continue to increase.

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Section: Growth Rate and Ec 50 Valuesmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Effect of 10 UV Filters on the Brine Shrimp Artemia salina and the Marine Microalga Tetraselmis sp.

Thorel

Clergeaud

Jaugeon

et al. 2020

Toxics

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“…This compound also causes photobleaching of corals and damage of aquatic ecosystem (Downs et al 2016). Mao et al (2017) showed that algae could absorb OBZ in culture solution, resulting in a decrease in chlorophyll (Chl) content and the inhibition of growth. Recently, the toxic effects of OBZ on organisms have attracted attention.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of photosynthesis in cucumber leaves by oxybenzone

Zhong¹,

Li²,

Zhang³

et al. 2020

Photosynt.

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Oxybenzone (OBZ), a widely used sunscreen additive, has damaging effects on corals and plants. This study demonstrated that OBZ significantly inhibited photosynthesis and growth of cucumber (Cucumis sativus L.) seedlings. Combined analyses of chlorophyll fluorescence and 820-nm reflection suggested that OBZ severely inhibited photosynthetic electron transport (PET) from QA to cytochrome b6f (Cyt b6f) complex, restricting the reduction of the reaction center in PSI. Separate measurements of PSI and PSII PET in chloroplasts indicated that OBZ inhibited the PET of neither PSII nor PSI, but it severely inhibited the whole linear PET. We thus confirmed that the inhibition of PET by OBZ was due to the inhibition of plastoquinones (PQs) and/or the Cyt b6f complex between PSI and PSII. PET is essential for producing ATP and NADPH used in CO2 assimilation. Therefore, limiting the use of sunscreens containing OBZ is significant in protection of aquatic and terrestrial ecosystems and ensuring the safety of agricultural production.

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“…Following the Organization of Economic Co-operation and Development (OECD) test guidelines [ 31 ], the strain was maintained in about 400 mL sterilized McBride Listeria agar (MLA) medium at an initial inoculation of 10% concentration ( V inoculum / V medium ) in several TPP tissue culture flasks (growth surface: 300 cm 2 ; with filter; TPP Techno Plastic Products AG, Trasadingen, Switzerland) [ 32 ]. The strain was kept in a constant temperature room (28 ± 1 °C) under cool white fluorescent lamps with a light intensity of 25 ± 2 μmol photons m −2 s −1 (12/12 h light/dark photoperiod) [ 21 ]. To ensure homogenous cell growth, the flasks were shaken and randomly relocated three times a day to eliminate the variations in light intensity at different positions.…”

Section: Methodsmentioning

confidence: 99%

“…In fact, various studies have tested the effects of BP-type UV filters on these organisms. From these studies, half maximal effective concentration (EC 50 ) values of BP-3 for green algae were estimated to be 0.25 mg L −1 for Skeletonema pseudocostatum , 0.96 mg L −1 for Desmodesmus subspicatus , 1.85 mg L −1 for Chlamydomonas reinhardtii and 22.4 mg L −1 for Chlorella vulgaris [ 18 , 19 , 20 , 21 ]. The EC 50 (72 h) of BP-1 was reported to be 10.5 mg L −1 for green alga, Raphidocelis subcapitata [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Joint Toxicity of Two Benzophenone-Type UV Filters on the Green Alga Chlamydomonas reinhardtii with Response Surface Methodology

Mao

Gin

2018

Toxics

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The widespread occurrence of benzophenone-type ultraviolet (UV) filter has raised the public concerns over the ecotoxicological effects of these chemicals. The present study assessed the joint toxicity of two representative benzophenones, benzophenone-1 (BP-1) and benzophenone-3 (BP-3), on the green alga Chlamydomonas reinhardtii using response surface methodologies (RSM). Specific growth rate and photosynthetic pigments were used as endpoints to evaluate the toxic effects. Generally, exposure to the combined BP-1 and BP-3 negatively affected cell growth and pigments production, with higher inhibitions at higher exposure concentrations. The simultaneous reduction in growth rate and pigments contents indicated that BP-1 and BP-3 regulated the growth of the tested alga by affecting the photosynthesis process. Results also showed that second order polynomial regression models fitted well with experimental results for all endpoints. The obtained regression models further indicated that the effects of the combination stemmed significantly from the linear concentration of BP-1 and BP-3. The overall results demonstrated that RSM could be a useful tool in ecotoxicological studies.

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Effects of benzophenone-3 on the green alga Chlamydomonas reinhardtii and the cyanobacterium Microcystis aeruginosa

Cited by 66 publications

References 46 publications

Effect of 10 UV Filters on the Brine Shrimp Artemia salina and the Marine Microalga Tetraselmis sp.

Effect of 10 UV Filters on the Brine Shrimp Artemia salina and the Marine Microalga Tetraselmis sp.

Inhibition of photosynthesis in cucumber leaves by oxybenzone

Evaluating the Joint Toxicity of Two Benzophenone-Type UV Filters on the Green Alga Chlamydomonas reinhardtii with Response Surface Methodology

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