Effects of Benzo(a)pyrene on the Expression of Heat Shock Proteins, Pro-inflammatory Cytokines and Antioxidant Enzymes in Hepatic Tumors Induced by Rat Hepatoma N1-S1 Cells

Zheng, Zhi; Park, So Young; Lee, Min; Phark, Sohee; Won, Nam Hee; Kang, Hyung Sik; Sul, Donggeun

doi:10.3346/jkms.2011.26.2.222

Cited by 5 publications

(4 citation statements)

References 28 publications

(26 reference statements)

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“…There was a high correlation between the overexpression of the potential prognostic factor, β-catenin, and the progression of colorectal cancer from normal epithelial tissue, polyps, and adenomas to carcinomas in colorectal cancer patients 12 13 and levels of SOD and IL-6 were closely related to cancer risk. 14 15 The results confirmed that oral intake of low doses-BaP caused harmful effects by inducing DNA adducts by increasing the inflammation (i.e., IL-6) and oxidative stress (i.e., SOD1) in the stomach and colon.…”

Section: Discussionsupporting

confidence: 58%

The Risk of Gastrointestinal Cancer on Daily Intake of Low-Dose BaP in C57BL/6 for 60 Days

Zheng

Park

Kwon³

et al. 2022

J Korean Med Sci

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Background Benzo(a)pyrene (BaP) is a carcinogenic compound in contaminated foodstuffs. The effect of oral intake of the environmental carcinogen BaP under low doses and frequent exposure on a digestive system has not been thoroughly verified. Methods In this regard, this study was conducted to prove the toxicity effects of BaP on the stomach and colon tissue after exposure to C57BL/6 mouse (3 and 6 µg/kg) following daily oral administration for 60 days. This study investigated acute gastric mucosal injury, severe gastric edema, cell infiltration, and mononuclear cells, multifocal cells, and tumoral inflammatory cells. Results The results of ELISA showed that the expression of serum interleukin (IL)-6 and tumor necrosis factor-α in the BaP exposure group were significantly increased, and a high level of DNA adduct distribution in their stomach and colon. Moreover, this study has confirmed the expression of early carcinogenesis markers: nuclear factor (NF)-κB, p53, IL-6, superoxide dismutase 1 (SOD1), mucin (MUC1 and MUC2), and β-catenin in the stomach and colon, and showed that there was a significant increase in IL-6, NF-κB, SOD1, β-catenin, and MUC1 ( P < 0.05). At the same time, there was a significant decrease in MUC2 and p53 ( P < 0.05). Thus, even in low doses, oral intake of BaP can induce DNA damage, increasing the potential risk of gastrointestinal cancer. Conclusion This study will provide a scientific basis for researching environmental contaminated food and intestinal health following daily oral administration of BaP.

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Section: Discussionsupporting

confidence: 58%

The Risk of Gastrointestinal Cancer on Daily Intake of Low-Dose BaP in C57BL/6 for 60 Days

Zheng

Park

Kwon³

et al. 2022

J Korean Med Sci

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“…The inflammation might be occurred when the production of ROS is excessive or sustained increasing and antioxidant enzymes cannot balance it . Zheng et al found that hepatic tumors induced by BaP expressed a higher protein expression of TNF‐α . A study also showed that diesel exhaust particles exposure increased the protein expression of brain capillary TNF‐α .…”

Section: Resultsmentioning

confidence: 99%

Effects of phenanthrene on oxidative stress and inflammation in lung and liver of female rats

Wang

Zhang

et al. 2019

Environmental Toxicology

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Phenanthrene (Phe) female rat model was established to explore the effects of Phe on oxidative stress and inflammation. The rats were randomly divided into three groups including control (C), low (L), and high (H) group. Phe was supplied to L and H groups at the dosage of 180 mg/kg and 900 mg/kg orally at first day, and with the dose 90 mg/kg and 450 mg/kg by intraperitoneal injection at the last 2 days. The C group was enriched with the same volume of corn oil. The blood, lung, and liver tissues were collected. The superoxide dismutase (SOD), malonaldehyde (MDA), and 8-hydroxy-2-deoxyguanosine (8-OHdG) were detected to evaluate oxidative stress.The protein and mRNA expressions of interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), and interleukin 10 (IL-10) were detected to evaluate inflammation. Further, the forkhead box transcription factor 3 (Foxp3) was analyzed to hint the injury mechanism of inflammation. The results showed SOD and MDA in lung and liver, and serum 8-OHdG elevated significantly in H groups (P < .05). Meanwhile, there were significant increases in the protein and mRNA expression of TNF-α and IL-6 in lung and liver of H groups (P < .05). In addition, the protein and mRNA expressions of TGF-β and Foxp3 were all decreased significantly in both lung and liver of H groups (P < .05). Results demonstrated that an obvious change of Phe exposure could induce oxidative stress and inflammation in female rats. This is a first pilot study to explore the association between Phe exposure and oxidative stress and inflammation using a female rat model. K E Y W O R D Sinflammatory cytokines, liver, lung, oxidative stress, phenanthrene

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“…These data reveal that the decreased DNA damage after BaP exposed-cells co-treated with curcumin or VE is not a result of the induction/inhibition of CYP genes involved in BaP metabolism. The Phase II enzyme, SOD, is essential for the elimination of ROS and studies have observed that in rats, a correlation between increased SOD expression and BaP exposure exists [53] and similarly, COMT has been known to detoxify B[a]P-7,8-dione [54] . The unchanged SOD-1 and COMT protein levels after BaP exposure observed in the present study suggest that other Phase II enzymes might be involved in the detoxification of BaP-induced damage, which requires further study.…”

Section: Discussionmentioning

confidence: 99%

Curcumin and Vitamin E Protect against Adverse Effects of Benzo[a]pyrene in Lung Epithelial Cells

et al. 2014

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Benzo[a]pyrene (BaP), a well-known environmental carcinogen, promotes oxidative stress and DNA damage. Curcumin and vitamin E (VE) have potent antioxidative activity that protects cells from oxidative stress and cellular damage. The objectives of the present study were to investigate the adverse effects of BaP on normal human lung epithelial cells (BEAS-2B), the potential protective effects of curcumin and VE against BaP-induced cellular damage, and the molecular mechanisms of action. MTT assay, flow cytometry, fluorescence microplate assay, HPLC, qRT-PCR, and western blot were performed to analyze cytotoxicity, cell cycle, reactive oxygen species (ROS), BaP diol-epoxidation (BPDE)-DNA adducts, gene expression, and protein expression, respectively. Curcumin or VE prevented cells from BaP-induced cell cycle arrest and growth inhibition, significantly suppressed BaP-induced ROS levels, and decreased BPDE-DNA adducts. While CYP1A1 and 1B1 were induced by BaP, these inductions were not significantly reduced by curcumin or VE. Moreover, the level of activated p53 and PARP-1 were significantly induced by BaP, whereas this induction was markedly reduced after curcumin and VE co-treatment. Survivin was significantly down-regulated by BaP, and curcumin significantly restored survivin expression in BaP-exposed cells. The ratio of Bax/Bcl-2 was also significantly increased in cells exposed to BaP and this increase was reversed by VE co-treatment. Taken together, BaP-induced cytotoxicity occurs through DNA damage, cell cycle arrest, ROS production, modulation of metabolizing enzymes, and the expression/activation of p53, PARP-1, survivin, and Bax/Bcl-2. Curcumin and VE could reverse some of these BaP-mediated alterations and therefore be effective natural compounds against the adverse effects of BaP in lung cells.

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Effects of Benzo(a)pyrene on the Expression of Heat Shock Proteins, Pro-inflammatory Cytokines and Antioxidant Enzymes in Hepatic Tumors Induced by Rat Hepatoma N1-S1 Cells

Cited by 5 publications

References 28 publications

The Risk of Gastrointestinal Cancer on Daily Intake of Low-Dose BaP in C57BL/6 for 60 Days

The Risk of Gastrointestinal Cancer on Daily Intake of Low-Dose BaP in C57BL/6 for 60 Days

Effects of phenanthrene on oxidative stress and inflammation in lung and liver of female rats

Curcumin and Vitamin E Protect against Adverse Effects of Benzo[a]pyrene in Lung Epithelial Cells

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