Effects of benzisoxazole and benzisothiazole on tomato plant regeneration in vitro

Branca, C.; Torelli, Anna; Bassi, Maria

doi:10.1007/bf00034486

Cited by 19 publications

(2 citation statements)

References 8 publications

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“…Twelve cotyledon explants, obtained from seedlings cultivated in vitro, were plated on MS medium (Murashige and Skoog 1962) containing the compounds instead of traditional cytokinins, at 1, 5 and 10 lM, both alone and in the presence of 20 lM 1,2-benzisoxazole-3-acetic acid (BOAA), as auxin (Branca et al 1990). Control was performed by cotyledon explants cultured in the presence of N-phenyl-N¢-1,2,3-thiadiazol-5-ylurea (thidiazuron, TDZ), as ureic cytokinin-like compound (Mok et al 1982), in the same culture conditions.…”

Section: Tomato Regeneration Testmentioning

confidence: 99%

Diheteroarylurea derivatives as adventitious rooting adjuvants in mung bean shoots and M26 apple rootstock

et al. 2006

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The present research investigates the biological profile of eight symmetrical diheteroarylureas and phenylheteroarylureas, testing their hypothetical cytokinin-like activity and rooting activity. Cytokinin-like activity was assayed by the betacyanin (so-called amaranthin) accumulation test and by the tomato regeneration test. The rooting activity was assessed using the mung bean rooting test, the apple stem slice test and the rooting of apple microcuttings. Three compounds, 1,3-di(pyrazin-2-yl)urea (3a), 1,3-di(benzo[d] oxazol-5-yl)urea (3b) and 1,3-di(benzo[d]oxazol-6-yl)urea (3c), enhanced adventitious root formation in apple stem slice test, but only 3b and 3c were active in the mung bean rooting test. Compound 3b, that showed the best rooting activity, was also able to enhance the adventitious root formation in apple microcuttings. None of the compounds showed cytokinin-like activity.

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Section: Tomato Regeneration Testmentioning

confidence: 99%

Diheteroarylurea derivatives as adventitious rooting adjuvants in mung bean shoots and M26 apple rootstock

et al. 2006

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“…Shoot determination was induced by in vitro culture in the presence of BOAA (a synthetic stereo-isomer of indole-acetic acid that has previously been proved to be particularly active in shoot induction) [5] plus ZR (BZ treatment) [6,7]. With the aim of distinguishing between genes specifically involved in shoot determination and genes mainly related to the stress resulting from explantation and in vitro culture, explants driven toward callus formation by a different hormonal treatment, 2,4-D plus zeatin riboside (DZ treatment), were utilized as 'non-organogenesis' references.…”

Section: Resultsmentioning

confidence: 99%

New potential markers of in vitro tomato morphogenesis identified by mRNA differential display

et al. 1996

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The identification of plant genes involved in early phases of in vitro morphogenesis can not only contribute to our understanding of the processes underlying growth regulator-controlled determination, but also provide novel markers for evaluating the outcome of in vitro regeneration experiments. To search for such genes and to monitor changes in gene expression accompanying in vitro regeneration, we have adapted the mRNA differential display technique to the comparative analysis of a model system of tomato cotyledons that can be driven selectively toward either shoot or callus formation by means of previously determined growth regulator supplementations. Hormone-independent transcriptional modulation (mainly down-regulation) has been found to be the most common event, indicating that a non-specific reprogramming of gene expression quantitatively predominates during the early phases of in vitro culture. However, cDNA fragments representative of genes that are either down-regulated or induced in a programme-specific manner could also be identified, and two of them (G35, G36) were further characterized. One of these cDNA fragments, G35, corresponds to an mRNA that is down-regulated much earlier in callus- (day 2) than in shoot-determined explants (day 6). The other, G36, identifies an mRNA that is transiently expressed in shoot-determined explants only, well before any macroscopic signs of differentiation become apparent, and thus exhibits typical features of a morphogenetic marker.

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1,2‐Benzisoxazoles

Gualtieri

Giannella

1999

Chemistry of Heterocyclic Compounds: A Series of Monographs

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Effects of benzisoxazole and benzisothiazole on tomato plant regeneration in vitro

Cited by 19 publications

References 8 publications

Diheteroarylurea derivatives as adventitious rooting adjuvants in mung bean shoots and M26 apple rootstock

Diheteroarylurea derivatives as adventitious rooting adjuvants in mung bean shoots and M26 apple rootstock

New potential markers of in vitro tomato morphogenesis identified by mRNA differential display

1,2‐Benzisoxazoles

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