Effects of Bavistin and Cefotaxime on in vitro Contaminant free Shoot Regeneration of Ruellia tuberosa L.

Abstract

In general, antimicrobial agents are often used in micropropagation techniques to obtain contaminant free clones. The objective of the present study was to evaluate the effects of bavistin and cefotaxime on producing contaminant free plants of Ruellia tuberosa cultured on MS supplemented with phytohormones. Field grown nodal explants of Ruellia tuberosa was used to regenerate entire plants via direct organogenesis. Among the decontaminants tested, the fungicide bavistin along with higher concentration of BAP (2.0 mg/l) and lower concentration of NAA (1.0 mg/l) was the most effective in regeneration and producing contaminant free shoots from cultured explants. This fungicide at 300 mg/l minimised fungal contamination with survival rate of 54%. While the addition of decontaminant cefotaxime at low concentration (200 mg/l) along with same concentration of BAP and NAA stimulated the bud formation and controlled the bacterial contamination. However, its increasing concentration adversely affected the survival rate of Ruellia tuberosa. These findings clearly showed that low concentrations of bavistin and cefotaxime were not only non-toxic but also facilitated bud regeneration. The results achieved showed the decisive role not only of the use of successful fungicides and antibiotics, but also of their sufficient doses were very important in reducing contamination and helping multiple shoot proliferation.
 Plant Tissue Cult. & Biotech. 31(1): 1-12, 2021 (June)

Studies on In vitro Surface Sterilisation and Antioxidants on Pomegranate (Punica granatum L.) cv. Bhagwa

“…Here, Carbendazim inhibits fungal contamination by reducing mycelial growth, germ tissue development, and appressoria formation and even interferes with fungal metabolism [28]. Similarly, fungal contamination was overcome in the tissue culture of pomegranate [16] and sugarcane variety Co 0118 [29]. Lakshmi et al [29] used a higher concentration of carbendazim (300 mg/L) to curb fungal contamination Finally, it inhibits the reproduction and further growth of the fungus [30].…”

“…Similarly, fungal contamination was overcome in the tissue culture of pomegranate [16] and sugarcane variety Co 0118 [29]. Lakshmi et al [29] used a higher concentration of carbendazim (300 mg/L) to curb fungal contamination Finally, it inhibits the reproduction and further growth of the fungus [30]. The use of tebuconazole as an effective systemic fungicide in explant surface sterilisation is rising [31;32].…”

Impact of Gibberellic Acid and Naphthalene Acetic Acid on Axillary Shoot Multiplication in Hygrophila polysperma (Roxb.) T. Anderson

“…(Dogan, 2018), Bacopa monnieri L. Pennell (Dogan & Emsen, 2018), Glycine max L. Merr. (Mangena, 2021), Ruellia tuberosa L. (Lakshmi et al, 2021), Paulownia tomentosa (Thunb.) Steud (Amirova et al, 2022), Vanilla borneensis Rolfe (Hasnu & Tanti, 2022) and Vaccinium oldhamii Miq.…”