Effects of basic fibroblast growth factor on the development of GABAergic neurons in culture

Deloulme, Jean-Christophe; Gensburger, C.; Sarhan, S.; Seiler, Nikolaus; Sensenbrenner, M.

doi:10.1016/0306-4522(91)90398-8

Cited by 21 publications

(6 citation statements)

References 27 publications

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“…A recent report from our laboratory has described that 6-day-old cul tures derived from 14-day-old fetal rat cerebral hemi spheres are highly enriched in GABAergic neurons and that a part of these neuronal cells are enhanced to prolif erate under the influence of bFGF [10]. Here, we found that another specific neuronal population, namely AChE-positive cells had ceased to proliferate under our culture conditions.…”

supporting

confidence: 50%

“…It has also been reported that bFGF influences dopaminergic and GABAergic neurons from rat mesencephalon [1]. We found that bFGF affects GABAergic neurons in cul tures of fetal rat cerebral hemispheres [10], In the present work we investigated in this culture system the effect of bFGF on the in vitro development of acetylcholinester ase (AChE)-containing neurons. The bFGF was added to the culture medium either at a time when ncuroblasts still proliferate (between 4 h and 2 days after culture initiation) or at a time when neuronal multiplication has ceased (between 2 and 4 days after culture initia tion) [11].…”

Section: Introductionmentioning

confidence: 98%

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Influence of Basic Fibroblast Growth Factor on the Development of Cholinoceptive Neurons from Fetal Rat Cerebrum in Culture

Gensburger¹,

Capo

Deloulme³

et al. 1992

Dev Neurosci

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Neuronal cells from cerebral hemispheres of 14-day-old rats were grown for 6 days in a serum-free, chemically defined medium. About 95–98 and 3% of these cells were neurofilament and acetylcholinesterase (AChE)-positive, respectively. The addition of basic fibroblast growth factor (bFGF) at three developmental stages, i.e. at 4 h, 2 and 4 days resulted in an increase (about 2-fold) of the number of AChE-positive neurons. The enzyme reaction was present in the cell body as well as in the fibers, which often ramified extensively under the influence of bFGF. Treatment with bFGF after the 2nd day of culture had no or only a low stimulatory effect. Our findings indicate that bFGF affects the development of AChE-containing neurons, i.e. cholinoceptive neurons from rat cerebrum.

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confidence: 50%

Section: Introductionmentioning

confidence: 98%

Influence of Basic Fibroblast Growth Factor on the Development of Cholinoceptive Neurons from Fetal Rat Cerebrum in Culture

Gensburger¹,

Capo

Deloulme³

et al. 1992

Dev Neurosci

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“…In the folded parts of the tectum, the laminae are consistently smooth and devoid of volcanoes. Discussion FGF2 is a secreted growth factor that in vitro prolongs proliferation and delays differentiation for various neuronal and glial precursors (25)(26)(27)(28). It also delays neurogenesis in vivo.…”

Section: Resultsmentioning

confidence: 99%

Expansion, folding, and abnormal lamination of the chick optic tectum after intraventricular injections of FGF2

McGowan

Alaama

Freise

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Comparative research has shown that evolutionary increases in brain region volumes often involve delays in neurogenesis. However, little is known about the influence of such changes on subsequent development. To get at this question, we injected FGF2-which delays cell cycle exit in mammalian neocortex-into the cerebral ventricles of chicks at embryonic day (ED) 4. This manipulation alters the development of the optic tectum dramatically. By ED7, the tectum of FGF2-treated birds is abnormally thin and has a reduced postmitotic layer, consistent with a delay in neurogenesis. FGF2 treatment also increases tectal volume and ventricular surface area, disturbs tectal lamination, and creates small discontinuities in the pia mater overlying the tectum. On ED12, the tectum is still larger in FGF2-treated embryos than in controls. However, lateral portions of the FGF2-treated tectum now exhibit volcano-like laminar disturbances that coincide with holes in the pia, and the caudomedial tectum exhibits prominent folds. To explain these observations, we propose that the tangential expansion of the ventricular surface in FGF2-treated tecta outpaces the expansion of the pial surface, creating abnormal mechanical stresses. Two alternative means of alleviating these stresses are tectal foliation and the formation of pial holes. The latter probably alter signaling gradients required for normal cell migration and may generate abnormal patterns of cerebrospinal fluid flow; both abnormalities would generate disturbances in tectal lamination. Overall, our findings suggest that evolutionary expansion of sheet-like, laminated brain regions requires a concomitant expansion of the pia mater.brain evolution | evolutionary developmental biology | proliferation | meninges | cortical folding E volutionary increases in brain region volumes are common (1).For example, the neocortex is disproportionately enlarged in primates relative to other mammals, and the telencephalon is disproportionately enlarged in parrots and songbirds relative to other birds (1-4). Recent work in evolutionary developmental neurobiology has shown that these evolutionary increases in brain region volumes are often caused by delays in cell cycle exit of neuronal precursors (5, 6). Among birds, for example, parrots and songbirds exhibit delayed telencephalic neurogenesis relative to chicken-like birds (7-9). Among mammals, cell cycle exit in the neocortex is similarly delayed in primates, which have a disproportionately enlarged neocortex (5, 10-12).Unfortunately, the downstream effects of delayed cell cycle exit on subsequent developmental processes and adult morphology remain poorly understood. One way to fill this gap in our knowledge is to experimentally recreate the key species differences in the laboratory by means of carefully selected developmental manipulations. A good example of this phenocopy approach was the creation of transgenic mice with a constitutively active form of β-catenin that prolongs proliferation, increases neocortical volume, and generates cortica...

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“…It is known that the cells destined to become the GABA neurons of the substantia nigra pars reticulata have the same birthdates and originate from the same area of the neuroepithelium as the dopamine neurons of the pars compacta (Hanaway et al, 1972;Altman and Bayer, I98 I). Therefore, it is likely that both types of precursors are present in our cultures and are dividing in response to bFGF, since there is evidence that bFGF is mitogenic to GABA precursors (Deloulme et al, 1991) and that bFGF increases the survival and neurite outgrowth of GABA neurons in cultures from E I4 rat mesencephalon (Ferrari et al, 1989, I99 I ).…”

Section: Discussionmentioning

confidence: 99%

Basic fibroblast growth factor increases division and delays differentiation of dopamine precursors in vitro

Bouvier

Mytilineou

1995

J. Neurosci.

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Growth factors have been shown to be involved in the regulation of division and differentiation of neuroepithelial cells. In the present study we examined the ability of various factors to influence the development of dopamine precursors. Primary neuronal cultures were prepared from the embryonic day 12 (E12) rat ventral mesencephalon, a time and place which coincides with the beginning of the birth of the dopamine neurons of the substantia nigra pars compacta. At low plating density in serum-free medium, the dopamine precursors divide for approximately 1 d in vitro. We report here that basic fibroblast growth factor (bFGF) can expand the period of dopamine precursor division at least until day 8 in culture, which is well beyond the normal division of these cells. This increase in cell division was accompanied by a delay in differentiation as compared to untreated control cultures. Upon differentiation, the high-affinity dopamine uptake values in bFGF-treated cultures were 20 times maximal control values. Mature dopamine neurons appeared at the same time as astrocytes, which may be playing a role in inducing dopamine neuron differentiation. IGF-I, GDNF, and EGF were unable to mimic the effect of bFGF on division and differentiation of dopamine precursors. Expanding in vitro the number of dopamine precursors provides tissue that may be suitable for transplantation in patients with Parkinson's disease.

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Effects of basic fibroblast growth factor on the development of GABAergic neurons in culture

Cited by 21 publications

References 27 publications

Influence of Basic Fibroblast Growth Factor on the Development of Cholinoceptive Neurons from Fetal Rat Cerebrum in Culture

Influence of Basic Fibroblast Growth Factor on the Development of Cholinoceptive Neurons from Fetal Rat Cerebrum in Culture

Expansion, folding, and abnormal lamination of the chick optic tectum after intraventricular injections of FGF2

Basic fibroblast growth factor increases division and delays differentiation of dopamine precursors in vitro

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