Effects of bacteriophage on inhibition and removal of mixed biofilm of enterohemorrhagic Escherichia coli O157:H7 and O91:H-

Shigemura, Kumiko; Duc, Hoang Minh; Shen, Chang; Huang, Hung Hsin; Sato, Jun; Masuda, Yoshito; Honjoh, Ken Ichi; Miyamoto, Takahisa

doi:10.1016/j.lwt.2020.109945

Cited by 11 publications

(6 citation statements)

References 27 publications

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“…Hence, the impact on human health and the economic losses have promoted the development of different approaches to control or remove the biofilms. In recent years, bacteriophages were applied as a tool to reduce biofilm formation in various pathogenic bacteria, such as Pseudomonas aeruginosa [ 27 , 28 , 29 , 30 , 31 ], Klebsiella pneumoniae [ 32 ], E. coli [ 33 , 34 , 35 , 36 , 37 ], and Staphylococcus epidermidis [ 38 ]. Thus, bacteriophages were tested in this study to treat biofilms produced by STEC strains.…”

Section: Discussionmentioning

confidence: 99%

Application of Bacteriophages on Shiga Toxin-Producing Escherichia coli (STEC) Biofilm

2021

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Shiga toxin-producing Escherichia coli are pathogenic bacteria able to form biofilms both on abiotic surfaces and on food, thus increasing risks for food consumers. Moreover, biofilms are difficult to remove and more resistant to antimicrobial agents compared to planktonic cells. Bacteriophages, natural predators of bacteria, can be used as an alternative to prevent biofilm formation or to remove pre-formed biofilm. In this work, four STEC able to produce biofilm were selected among 31 different strains and tested against single bacteriophages and two-phage cocktails. Results showed that our phages were able to reduce biofilm formation by 43.46% both when used as single phage preparation and as a cocktail formulation. Since one of the two cocktails had a slightly better performance, it was used to remove pre-existing biofilms. In this case, the phages were unable to destroy the biofilms and reduce the number of bacterial cells. Our data confirm that preventing biofilm formation in a food plant is better than trying to remove a preformed biofilm and the continuous presence of bacteriophages in the process environment could reduce the number of bacteria able to form biofilms and therefore improve the food safety.

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Section: Discussionmentioning

confidence: 99%

Application of Bacteriophages on Shiga Toxin-Producing Escherichia coli (STEC) Biofilm

2021

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“…The treatment with phage AZO145A significantly inhibited the biofilm formation of STEC on the surface of stainless-steel plate, and significantly inhibited their migration at 24 °C in the biofilm formed in beef, which was reduced to 3.1 log 10 CFU, thereby reducing the risk of food cross infection [ 45 ]. At the same time, with the in-depth study of bacteriophages, increasingly more bacteriophages were found to have a good inhibitory effect on E. coli biofilm, such as bacteriophage FP43, Daica, and135 [ 27 , 46 ]. In the bacteriostatic effect of phage, phage cocktails have a better inhibition effect on biofilm than that of single phage.…”

Section: Control Measures For the Prevention Of E Coli ...mentioning

confidence: 99%

Control Measurements of Escherichia coli Biofilm: A Review

Zhou

Wang

et al. 2022

Foods

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Escherichia coli (E. coli) is a common pathogen that causes diarrhea in humans and animals. In particular, E. coli can easily form biofilm on the surface of living or non-living carriers, which can lead to the cross-contamination of food. This review mainly summarizes the formation process of E. coli biofilm, the prevalence of biofilm in the food industry, and inhibition methods of E. coli biofilm, including chemical and physical methods, and inhibition by bioactive extracts from plants and animals. This review aims to provide a basis for the prevention and control of E. coli biofilm in the food industry.

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“…Previous research found that phage FP43 was active against planktonic bacterial cells and inhibited and degraded a biofilm of E. coli O157:H7 and O19:H-. This phage decreased the formation of biofilms up to 82.4% 17 . DW-EC is also known to inhibit the growth of bacterial cells so that they cannot reach the required density to form biofilms.…”

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confidence: 92%

“…These results were higher than the phage DW-EC with a titer of 10 7 PFU/mL which had the highest inhibitory and destruction activities at 44.06% and 48.13%, respectively 20 . Furthermore, a recent report showed phage FP43 with a titer of 10 10 PFU/mL inhibited the formation of biofilm E. coli O157:H7 and O19:H-up to 82.4% 17 . The concentration of phage titer may affect the effectiveness of phage www.nature.com/scientificreports/ in reducing their activity to form and destroy biofilms.…”

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confidence: 95%

Bacteriophage DW-EC with the capability to destruct and inhibit biofilm formed by several pathogenic bacteria

Wiguna

Waturangi

Yogiara

2022

Sci Rep

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Biofilm formation by pathogenic bacteria is a major challenge in the food industry. Once a biofilm is established, such as on food processing equipment, it becomes more difficult to eradicate. Although physical and chemical treatments are often used to control biofilm formation, these treatments can have significant drawbacks. Alternative biofilm treatments are needed. Phage DW-EC was isolated from dawet, an Indonesian traditional Ready-To-Eat food, which has high specificity for Enterohaemorrhagic Escherichia coli (EHEC), Enteropathogenic E. coli (EPEC), and Enterotoxigenic E. coli (ETEC). Phage DW-EC produces several enzymes that can prevent the development of biofilm and biofilm eradication. Depolymerase enzymes break down the polysaccharides layer on the biofilms can lead to biofilm damage. On the other hand, endolysin and putative like-T4 lysozyme will lyse and kill a bacterial cell, thereby preventing biofilm growth. This research aims to determine the capability of previously identified phage DW-EC to inhibit and destroy biofilms produced by several foodborne pathogens. Phage DW-EC formed plaques on the bacterial lawns of EHEC, EPEC, and ETEC. The efficiency of plating (EOP) values for EHEC, EPEC, ETEC, and Bacillus cereus were 1.06, 0.78. 0.70, and 0.00, demonstrating that DW-EC was effective in controlling pathogenic E. coli populations. Furthermore, phage DW-EC showed anti-biofilm activity against foodborne pathogenic bacteria on polystyrene and stainless-steel substrates. DW-EC biofilm inhibition and destruction activities against pathogenic E. coli were significantly higher than against B. cereus biofilms, which was indicated by a lower density of the biofilm than B. cereus. Microscopic visualization verified that bacteriophage DW-EC effectively controlled EHEC, EPEC, and ETEC biofilms. The results showed that DW-EC could inhibit and destroy biofilm, making it promising to be used as an anti-biofilm candidate for polystyrene and stainless steel equipment in the food industry.

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Effects of bacteriophage on inhibition and removal of mixed biofilm of enterohemorrhagic Escherichia coli O157:H7 and O91:H-

Cited by 11 publications

References 27 publications

Application of Bacteriophages on Shiga Toxin-Producing Escherichia coli (STEC) Biofilm

Application of Bacteriophages on Shiga Toxin-Producing Escherichia coli (STEC) Biofilm

Control Measurements of Escherichia coli Biofilm: A Review

Bacteriophage DW-EC with the capability to destruct and inhibit biofilm formed by several pathogenic bacteria

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