Effects of bacterial endophytes from potato roots and tubers on potato cyst nematode (Globodera rostochiensis)

Abstract

Istifadah N, Pratama N, Taqwim S, Sunarto T. 2018. Effects of bacterial endophytes from potato roots and tubers on potato cyst nematode (Globodera rostochiensis). Biodiversitas 19: 47-51. Bacterial endophytes are bacteria that inhabit plant tissues without causing any diseases. The endophytes existence may have negative, neutral, or positive effects on the host plants. This paper discusses the effects of bacterial endophytes isolated from potato roots and tubers on potato growth and their abilities to suppress potato cyst nematode, Globodera rostochiensis. The bacterial endophytes were isolated from roots and tubers of potatoes obtained from six plantation areas in West Java. The endophyte isolates were examined for their effects on potato growth. The non-pathogenic isolates were tested for their abilities to suppress G. rostochiensis in vitro and in potato plants. The results showed that from 88 bacterial endophyte isolates obtained, 13 isolates caused rot in potato seed pieces, 22 isolates inhibited the potato growth, while, 2 isolates increased the growth, and as many as 51 isolates did not influence the growth. The in vitro test using the isolate culture filtrate revealed that there were seven isolates that caused mortality of G. rostochiensis juvenile-2 by 67.5-97.7%. These isolates, however, were not effective in damaging the nematode eggs. In the greenhouse experiment, the bacterial endophyte isolates suppressed the number of cysts by 51.7-65.4% and that of the juvenile-2 of G. rostochiensis by 48.6-76.4%.

The efficacy of compost, vermicompost and their combination with antagonistic microbes to reduce potato cyst nematode (Globodera rostochiensis)

“…and P. pseudoalcaligens effectively suppressed all kinds of G. rostochiensis propagules. This result confirmed previous studies that also found these microbes significantly reduced the numbers of second stage juveniles and cysts of G. rostochiensis in the soil (Istifadah et al, 2017;Istifadah et al, 2018). In the in vitro test, the culture filtrate of P. pseudoalcaligens also caused high mortality (97.9%) of second-stage of the nematode.…”

“…The filtrate of the bacteria caused lysis of the juvenile cell walls, indicating the presence of cell wall degrading enzyme (Istifadah et al 2018).…”

“…To support biological control of potato cyst nematode, Istifadah et al (2017) isolated endophytic fungi from potato roots and tubers and found one isolate, Paecilomyces sp., that significantly reduced G. rostochiensis inocula. Istifadah et al, (2018) also obtained one isolate of bacterial endophytes, Pseudomonas pseudoalcaligens, that effectively suppressed the numbers of G. rostochiensis cysts and the second-stage juveniles in the soil.…”

Fungal and Bacterial Endophytes as Microbial Control Agents for Plant-Parasitic Nematodes

“…Another study demonstrated significant control of M. incognita in potato from R. etli G12 application with 34% fewer galls than in control [ 64 ]. Bacillus carotarum , B. cereus , and Pseudomonas pseudoalcaligenes isolated from the roots and tubers of potato caused ≥80% mortality of Globodera rostochiensis J2s in vitro but did not limit the egg-hatch [ 66 ].…”

Sustainable Food Production Systems for Climate Change Mitigation: Indigenous Rhizobacteria for Potato Bio-fertilization in Tanzania

“…Contrary to the expectation, E. ludwigii BUMS resulted in average potato shoot length and weight that were less than those of the un-inoculated control by 3.94% and 5.94%, respectively. The failure of rhizobacterial inocula to produce the desired results during in planta investigations is probably due to the inabilities to establish themselves in the rhizosphere (Istifadah et al 2018).…”