Effects of Bacillus subtilis iturin A on HepG2 cells in vitro and vivo

Zhao, Haobin; Lü, Yan; Guo, Ling Jun; Sun, Hui; Huang, Qingsheng; Shao, Dongyan; Jiang, Chunmei; Shi, Junling

doi:10.1186/s13568-021-01226-4

Cited by 20 publications

(13 citation statements)

References 39 publications

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“…Fengycin, a lipopeptide produced by various strains of B. subtilis, was reported to exert strong anticancer activity on colon cancer cell line HT29 and human lung cancer cell line 95D [162,163]. Similarly, Bacillus lipopeptide (iturin) was also reported to possess a broad spectrum of anticancer activity on several cell lines (e.g., HepG2, Caco-2, BT474, MDA-MB-231, MCF-7, HUVEC, BIU-87, BRL-3A, A549, and K562 cells [42,[164][165][166][167][168][169][170][171].…”

Section: Pharmacological Activitiesmentioning

confidence: 99%

Lipopeptide Biosurfactants from Bacillus spp.: Types, Production, Biological Activities, and Applications in Food

Ali

Pang

Wang

et al. 2022

Journal of Food Quality

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Biosurfactants are a functionally and structurally heterogeneous group of biomolecules produced by multiple filamentous fungi, yeast, and bacteria, and characterized by their distinct surface and emulsifying ability. The genus Bacillus is well studied for biosurfactant production as it produces various types of lipopeptides, for example, lichenysins, bacillomycin, fengycins, and surfactins. Bacillus lipopeptides possess a broad spectrum of biological activities such as antimicrobial, antitumor, immunosuppressant, and antidiabetic, in addition to their use in skincare. Moreover, Bacillus lipopeptides are also involved in various food products to increase the antimicrobial, surfactant, and emulsification impact. From the previously published articles, it can be concluded that biosurfactants have strong potential to be used in food, healthcare, and agriculture. In this review article, we discuss the versatile functions of lipopeptide Bacillus species with particular emphasis on the biological activities and their applications in food.

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Section: Pharmacological Activitiesmentioning

confidence: 99%

Lipopeptide Biosurfactants from Bacillus spp.: Types, Production, Biological Activities, and Applications in Food

Ali

Pang

Wang

et al. 2022

Journal of Food Quality

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“…Although Iturin A rescued the growth of piglets challenged by STb-R in our study, the growth-promoting effect on healthy piglets was not significant, probably due to the short duration of administration, and the effect of long-term administration of Iturin A remains to be studied. A similar study reported that Iturin A administrated for 15 d significantly inhibited tumor growth; however, there were no significant effects on body weight, food intake, and viscera index in mice [ 24 ]. Taken together, Iturin A maybe better at resisting damaging stimulations.…”

Section: Discussionmentioning

confidence: 99%

Iturin A Rescued STb-R-Induced Pork Skeletal Muscle Growth Restriction through the Hypothalamic-Pituitary-mTORC1 Growth Axis

Liu

Gao

et al. 2022

Animals

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The engineered STb-Rosetta Escherichia coli (STb-R) was designed to investigate the effects of Iturin A on the skeletal muscle growth of weaned piglets. A total of 28 piglets were randomly divided into 4 groups (7 piglets per group): the control group (100 mL PBS), the Iturin A group (100 mL 320 mg/kg body weight (BW) Iturin A), the STb-R group (100 mL 1 × 1010 CFU/mL STb-R), and the Iturin A + STb-R group (100 mL 320 mg/kg BW Iturin A + 1 × 1010 CFU/mL STb-R). Compared with the control, STb-R-reduced body weight gain were rescued by Iturin A. The semimembranosus muscle weight recovered to normal level in the Iturin A + STb-R group. The level of relevant genes of the growth axis were elevated by Iturin A, including GHRH in the hypothalamus, GHRHR and GH in the pituitary, and GHR, IGF-1 and IGF-1R in the semimembranosus muscle. Moreover, Iturin A increased the mean fiber area and the number of proliferating cells in the semimembranosus muscle, which were decreased by STb-R. Additionally, the mTORC1 pathway was reactivated by Iturin A to relieve the suppression of STb-R. Collectively, the hypothalamic-pituitary growth axis-mediated Iturin A reactivated the mTORC1 pathway to rescue STb-R-restricted pork skeletal muscle growth.

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“…The control experiment showed less consistent ALT and AST release than DMSO. The release of higher concentrations of ALT and AST are associated with subsequent damage to the hepatocellular carcinoma cells [34]. The plant extracts of A. cappadocicum increased ALT and AST in the liver tumor MPS, representing the plant extracts' efficacy against the hepatocellular carcinoma cells.…”

Section: Effect Of a Cappadocicum On Liver Function Testsmentioning

confidence: 96%

Study of the Anticancer Potential of Plant Extracts Using Liver Tumor Microphysiological System

Farooqi

Sammantasinghar

Kausar

et al. 2022

Life

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Background: Plants have been considered a vital source of modern pharmaceutics since the paleolithic age. Contemporary chemotherapeutic drugs for cancer therapy are chemical entities sourced from plants. However, synthetic drugs or their derivatives come with severe to moderate side effects for human health. Hence, the quest to explore and discover plant-based novel anticancer drugs is ongoing. Anticancer activities are the primary method to estimate the potential and efficacy of an extract or compound for drug discovery. However, traditional in vitro anticancer activity assays often show poor efficacy due to the lack of in-vivo-like cellular environment. In comparison, the animal-based in vivo assays lack human genetic makeup and have ethical concerns. Aim: This study aimed to overcome the limitations of traditional cell-culture-based anticancer assays and find the most suitable assay for anticancer activity of plant extracts. We first reported utilizing a liver tumor microphysiological system in the anticancer effect assessment of plant extracts. Methodology: Methanolic extracts of Acer cappadocicum Gled were used to assess anticancer activity against liver tumor microphysiological system (MPS), and cell viability, liver function tests, and antioxidant enzyme activities were performed. Additionally, an embedded transepithelial electrical resistance sensor was utilized for the real-time monitoring of the liver tumor MPS. The results were also compared with the traditional cell culture model. Results: The study demonstrated the superiority of the TEER sensor-based liver tumor MPS by its better anticancer activity based on cell viability and biomarker analysis compared to the traditional in vitro cell culture model. The anticancer effects of the plant extracts were successfully observed in real time, and methanolic extracts of Acer cappadocicum Gled increased the alanine transaminase and aspartate aminotransferase secretion, which may reveal the different mechanisms of these extracts and suggest a clue for the future molecular study of the anticancer pathways. Conclusion: Our results show that the liver tumor microphysiological system could be a better platform for plant-based anticancer activity assessment than traditional cell culture models.

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Effects of Bacillus subtilis iturin A on HepG2 cells in vitro and vivo

Cited by 20 publications

References 39 publications

Lipopeptide Biosurfactants from Bacillus spp.: Types, Production, Biological Activities, and Applications in Food

Lipopeptide Biosurfactants from Bacillus spp.: Types, Production, Biological Activities, and Applications in Food

Iturin A Rescued STb-R-Induced Pork Skeletal Muscle Growth Restriction through the Hypothalamic-Pituitary-mTORC1 Growth Axis

Study of the Anticancer Potential of Plant Extracts Using Liver Tumor Microphysiological System

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