Effects of auxins and thiamine on the efficacy of techniques of clonal propagation in Jatropha curcas L.

Dhillon, R. S.; Hooda, M. S.; Pundeer, J. S.; Ahlawat, K. S.; Chopra, Indu

doi:10.1016/j.biombioe.2010.12.017

Cited by 15 publications

(9 citation statements)

References 17 publications

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“…[ 59 ]. Vitamin B1 is involved in the conversion from pyruvate to acetyl-COA on energy production as co-enzyme and it is also one of an important factor for plant root growth [ 46 , 60 ]. The energy metabolism linked from glycolysis to TCA cycle of FECs might be delay on FEC cultured on CIM-Ρ NPK media.…”

Section: Resultsmentioning

confidence: 99%

Formation of friable embryogenic callus in cassava is enhanced under conditions of reduced nitrate, potassium and phosphate

Utsumi

Tanaka

et al. 2017

PLoS ONE

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Agrobacterium-mediated transformation is an important research tool for the genetic improvement of cassava. The induction of friable embryogenic callus (FEC) is considered as a key step in cassava transformation. In the present study, the media composition was optimized for enhancing the FEC induction, and the effect of the optimized medium on gene expression was evaluated. In relative comparison to MS medium, results demonstrated that using a medium with reducing nutrition (a 10-fold less concentration of nitrogen, potassium, and phosphate), the increased amount of vitamin B1 (10 mg/L) and the use of picrolam led to reprogram non-FEC to FEC. Gene expression analyses revealed that FEC on modified media increased the expression of genes related to the regulation of polysaccharide biosynthesis and breakdown of cell wall components in comparison to FEC on normal CIM media, whereas the gene expression associated with energy flux was not dramatically altered. It is hypothesized that we reprogram non-FEC to FEC under low nitrogen, potassium and phosphate and high vitamin B1. These findings were more effective in inducing FEC formation than the previous protocol. It might contribute to development of an efficient transformation strategy in cassava.

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Section: Resultsmentioning

confidence: 99%

Formation of friable embryogenic callus in cassava is enhanced under conditions of reduced nitrate, potassium and phosphate

Utsumi

Tanaka

et al. 2017

PLoS ONE

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“…In self-incompatible species, propagation techniques might be the only option for working with genotypes or ecotypes of interest. Greenhouse propagation can take months to several years [20] [75]- [81], even with added growth hormones [75] [80]. Given the issues with genetic diversity studies using traditional methods and the evidence we present above, we suggest that micropropagation is a useful alternative.…”

Section: Discussionmentioning

confidence: 96%

Thinking inside the Box: Tissue Culture for Plant Propagation in a Key Ecological Species, <i>Andropogon gerardii</i>

Hoffman¹,

Smith²

2018

AJPS

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Intraspecific diversity has widespread effects on ecological communities and ecosystems. To elucidate the mechanisms underlying these effects, manipulative studies require a rigorous and efficient empirical approach. Yet, replicating sufficient numbers of genetically identical individuals remains a challenge. As a result, we are limited in our understanding of the mechanisms underlying the ecological effects of intraspecific diversity. In contrast, large sample sizes are routinely produced in horticultural research using micropropagation, or tissue culture. In order to determine the potential usefulness of micropropagation technique for ecological studies, we investigated the efficiency and efficacy of micropropagation on the ecologically important non-model C4 grass species, Andropogon gerardii. Our preliminary results demonstrate that micropropagation is a rapid and effective technique for producing large numbers of genetically identical clones at up to 100 times the rate of traditional propagation. Key intraspecific differences among clones of A. gerardii were also retained through the micropropagation process. Given that traditional techniques used to test the effects of intraspecific diversity manipulations are time-limiting (greenhouse propagation) or can be biologically misrepresentative (seeds) for some species, we suggest that micropropagation might be a powerful tool for advancing ecological genetics studies in many plant systems.

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“…Callus production impeded plantlet survival as callus creates an incomplete and weak connection between roots and shoots. In contrast, the presence of root hairs in plantlets supplemented with IBA aided in water absorption and translocation (Dhillon et al 2011). Although plantlet size did not significantly affect root count, root length, and root color (Table 1), survival was significantly higher (p = 0.04) in medium plantlets.…”

Section: Effect Of Plantlet Size and Pgr On In Vitro Root Developmentmentioning

confidence: 94%

In vitro rooting of Nepenthes truncata Macf.

Rasco¹,

Oguis²,

Silvosa³

2012

Carniv. Pl. Newslett.

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Effects of auxins and thiamine on the efficacy of techniques of clonal propagation in Jatropha curcas L.

Cited by 15 publications

References 17 publications

Formation of friable embryogenic callus in cassava is enhanced under conditions of reduced nitrate, potassium and phosphate

Formation of friable embryogenic callus in cassava is enhanced under conditions of reduced nitrate, potassium and phosphate

Thinking inside the Box: Tissue Culture for Plant Propagation in a Key Ecological Species, <i>Andropogon gerardii</i>

In vitro rooting of Nepenthes truncata Macf.

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Effects of auxins and thiamine on the efficacy of techniques of clonal propagation in Jatropha curcas L.

Cited by 15 publications

References 17 publications

Formation of friable embryogenic callus in cassava is enhanced under conditions of reduced nitrate, potassium and phosphate

Formation of friable embryogenic callus in cassava is enhanced under conditions of reduced nitrate, potassium and phosphate

Thinking inside the Box: Tissue Culture for Plant Propagation in a Key Ecological Species, &lt;i&gt;Andropogon gerardii&lt;/i&gt;

In vitro rooting of Nepenthes truncata Macf.

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Thinking inside the Box: Tissue Culture for Plant Propagation in a Key Ecological Species, <i>Andropogon gerardii</i>