Effects of atrazine on female Wistar rats: Morphological alterations in ovarian follicles and immunocytochemical labeling of 90kDa heat shock protein

Juliani, C.C.; Silva-Zacarín, Elaine C.M.; Santos, Daniela Cristina dos; Bôer, Patrícia Aline

doi:10.1016/j.micron.2007.04.006

Cited by 38 publications

(26 citation statements)

References 30 publications

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“…Follicles were classified as either healthy (intact basallamina, oocyte with no more than 3 cytoplasmic vacuoles, intact germinal vesicle and nucleolus) or atretic (apoptotic). Antral follicles were considered atretic if they contained at least 20 apoptotic granulosa cells (defined by the appearance of apoptotic bodies in the granulosa cell layer), disorganized granulosa cells, a degenerating oocyte, or a fragmentation of the oocyte nucleus (Juliani et al 2008). …”

Section: Follicular Classificationmentioning

confidence: 99%

Effects of plant polyphenols on ovarian follicular reserve in aging ratsThis paper is one of a selection of papers published in this special issue entitled “Second International Symposium on Recent Advances in Basic, Clinical, and Social Medicine” and has undergone the Journal's usual peer review process.

Chen

Luo

et al. 2010

Biochem. Cell Biol.

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The pool of ovarian primordial follicles is established during embryonic development or at birth. During the development from primordial to primary, secondary, and antral follicles, only a small portion of follicles can mature and successfully ovulate; the others are destined to degenerate through apoptotic or atretic loss. As aging advances, females ultimately enter the cessation phase of the estrous cycle and are no longer capable of fertilization. The presumption is that if we can slow down the process of folliculogenesis or decrease follicle loss, females may have a larger ovarian follicular reserve and a longer reproductive lifespan. In our study, rats underwent intragastric administration with tea polyphenols, quercetin (meletin), genistein, or resveratrol, once a day for 4 months (from age 12 to 15 months), to test whether they have positive effects on follicular reserve or ovarian functions. The results showed that rats treated with tea polyphenols (27.8 +/- 3.2) and quercetin (36.5 +/- 4.1) had a comparable number of healthy follicles to those of controls (26.9 +/- 3.8), although significantly fewer atretic follicles were observed in the tea polyphenol group (43.4 +/- 5.9 vs 79.7 +/- 7.5; p < 0.001). Remarkably, both genistein- and resveratrol-treated rats had more healthy follicles (respectively, 42.8 +/- 3.9, p < 0.05; and 51.9 +/- 6.4, p < 0.001) and fewer atretic follicles (respectively, 58.4 +/- 8.0, p < 0.05; and 51.0 +/- 6.2, p < 0.01) than controls. These results indicate that genistein and resveratrol can increase the ovarian follicular reserve and prolong the ovarian lifespan in rats, and their positive effects may be not only due to their intervention in the transition from primordial to primary follicle, but also due to the inhibiting effect on follicular atresia.

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Section: Follicular Classificationmentioning

confidence: 99%

Effects of plant polyphenols on ovarian follicular reserve in aging ratsThis paper is one of a selection of papers published in this special issue entitled “Second International Symposium on Recent Advances in Basic, Clinical, and Social Medicine” and has undergone the Journal's usual peer review process.

Chen

Luo

et al. 2010

Biochem. Cell Biol.

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“…Specifically, chronic exposure to ATZ during adulthood impairs folliculogenesis in female rats and leads to increased follicle atresia and the production of multi-oocyte follicles (MOF) 19 . The changes in follicular morphology were associated with abnormal endocrine function.…”

Section: Introductionmentioning

confidence: 99%

Embryonic exposure to the widely-used herbicide atrazine disrupts meiosis and normal follicle formation in female mice

Gély-Pernot

Saci

Kernanec

et al. 2017

Sci Rep

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The widely-used herbicide atrazine (ATZ) is detected in ground and surface water in many countries. Several studies in animals have demonstrated that ATZ has endocrine-disrupting effects on male and female reproduction in many vertebrate species. In this study, we investigated the effects of ATZ exposure on meiosis, a key step in gametogenesis in mammals. The treatment was initiated before oocyte entry into meiosis, which occurs during the embryonic period in females. We found that embryonic exposure to ATZ increases the level of 8-oxo-guanine in the nucleus of meiotic cells, reflecting oxidative stress and affecting meiotic double-strand break repair, chromosome synapsis and crossover numbers. Finally, embryonic exposure to ATZ reduces the number of primordial follicles and increases the incidence of multi-oocyte follicles in adult mice. Our data demonstrate that embryonic exposure to ATZ disrupts prophase I of meiosis and affects normal follicle formation in female mice.

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“…Additionally to histophatological analysis of rat ovaries (Table 1), our studies also emphasized the immunohistochemical labeling of 90 KD heat shock protein (HSP 90) in order to evaluate the role played by this protein in the ovary, under stressed conditions induced by ATZ exposure. Our results indicated that atrazine induced impaired folliculogenesis, increased follicular atresia and HSP90 depletion in female rats submitted to subacute treatment, while the subchronic treatment with the lowest dose of ATZ could compromise the reproductive capacity reflected by the presence of multioocytic follicle and stress-inducible HSP90 (Juliani et al, 2008).…”

Section: Reproductive Toxicitymentioning

confidence: 69%

Effects of Herbicide Atrazine in Experimental Animal Models

Severi-Aguiar¹,

Silva-Zacarín²

2012

Herbicides - Properties, Synthesis and Control of Weeds

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Effects of atrazine on female Wistar rats: Morphological alterations in ovarian follicles and immunocytochemical labeling of 90kDa heat shock protein

Cited by 38 publications

References 30 publications

Embryonic exposure to the widely-used herbicide atrazine disrupts meiosis and normal follicle formation in female mice

Effects of Herbicide Atrazine in Experimental Animal Models

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