Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma

Wang, Gang; Liu, Chun‐Tao; Wang, Zengli; Yan, Chaowu; Luo, Fengming; Wang, Lei; Li, Ting-qian

doi:10.1007/s11655-006-0262-y

Cited by 15 publications

(7 citation statements)

References 22 publications

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“…Higher levels of IL-1, IL-6, IL-8, and TNF␣ were found in children with nephrotic syndrome when treated with A. membranaceus (Peng et al 2005). In asthmatic patients treated with A. membranaceus, the mRNA expression of the Th1 transcription factor T box in T cells was significantly increased and the level of IL-4 was decreased (Wang et al 2006). Although controversial results exist regarding the treatment of allergic patients with A. membranaceus, most reports favor its Th1-polarizing effect on asthma, cancer, herpes simplex keratitis, or type I diabetes (Tomas 2011).…”

Section: Fig 2 Effects Of a Membranaceus On The Antibody Levels Ofmentioning

confidence: 99%

Astragalusmembranaceusmodulates Th1/2 immune balance and activates PPARγ in a murine asthma model

Chen

Tsai

Lee

et al. 2014

Biochem. Cell Biol.

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Astragalus membranaceus, a traditional Chinese herb, has been used to improve airway inflammation and asthma. The present study investigated whether A. membranaceus has immunotherapeutic effects on asthma, a chronic inflammatory mucosal disease that is associated with excess production of IgE, eosinophilia, T helper 2 (Th2) cytokines, and bronchial hyperresponsiveness. An ovalbumin (OVA)-induced, chronic inflammatory airway murine asthma model was used to examine the status of pulmonary inflammation after the administration of A. membranaceus. The IgE levels in serum and bronchoalveolar lavage fluid showed a tendency to decrease after the administration of A. membranaceus. The number of eosinophils decreased and infiltration of inflammatory cells and collagen deposition declined in lung sections after A. membranaceus administration. The RNA and protein levels of Th2 cytokines and the ratio of the GATA3/T-bet mRNA levels decreased after A. membranaceus treatment. Furthermore, the mRNA level of peroxisome proliferator-activated receptor γ (PPARγ), a nuclear hormone receptor, increased in the lung tissues of A. membranaceus-treated mice. Finally, an A. membranaceus water extract activated PPARγ activity in either human embryonic kidney 293 (HEK293) or A549 cells in a PPARγ-responsive element-containing luciferase reporter assay. These results indicate that A. membranaceus has an inhibitory effect on airway inflammation in a murine model of asthma through modulating the imbalanced relationship between Th1 and Th2 cytokines.

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Section: Fig 2 Effects Of a Membranaceus On The Antibody Levels Ofmentioning

confidence: 99%

Astragalusmembranaceusmodulates Th1/2 immune balance and activates PPARγ in a murine asthma model

Chen

Tsai

Lee

et al. 2014

Biochem. Cell Biol.

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“…Many studies have found that AM has the obvious therapeutic effect on allergic diseases, such as asthma and AR. 20–22 Due to its complex therapeutic mechanism, extensive studies and reports are still lacking. Therefore, we mainly studied the role of AM in the mouse model of AR.…”

Section: Discussionmentioning

confidence: 99%

“…18 In addition, Yang et al reported AM raising the immune response of the host organism to against tumor activity of liver cancer. 19 Beyond that, although AM has long been used in the treatment of asthma and AR, 20,21 the mechanism for treating AR remains unclear. Therefore, we hereby investigate the anti-inflammatory effect of AM and whether this effect is associated with the CD4 þ CD25 þ Foxp3 þ T cells and NF-jB pathway.…”

Section: Introductionmentioning

confidence: 99%

Effect of Astragalus membranaceus in Ovalbumin-Induced Allergic Rhinitis Mouse Model

Zhong

Jin-Tao

Liu

et al. 2019

Am J Rhinol�Allergy

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Background Astragalus membranaceus (AM), a traditional Chinese medicine, has been used to treat allergic diseases, but the mechanism for treating allergic rhinitis (AR) remains unclear. Objective The purpose of this study was to look at the anti-inflammatory effect of AM on AR and the mechanism of anti-allergy. Methods The mouse model of AR was induced by ovalbumin. Allergic symptoms, number of eosinophils in nasal mucosa, and levels of inflammatory cells in nasal lavage fluid were analyzed. We explored the serum immunoglobulin E (IgE), interleukin-4 (IL-4), IL-5, IL-13, interferon-γ (IFN-γ), and IL-10. Besides, the relative mRNA of IL-4, IL-5, and IL-13 was also detected in nasal mucosa tissue. The proportion of CD4+ CD25+ Foxp3+ T cells in the spleen and nasal lymphoid tissue were analyzed. The mRNA levels of nuclear factor-kappa B p65 (NF-κB p65) and inhibitory kappa B alpha (IκBα), as well as NF-κB p65 DNA binding activity, were tested. We also measured the protein levels of NF-κB p65 and p-NF-κB p65 in nasal mucosa. Results AM could reduce the number of eosinophils in the nasal mucosa and decrease the levels of inflammatory cells in nasal lavage fluid. The serum IgE, IL-4, IL-5, and IL-13 were also decreased, while levels of IFN-γ and IL-10 were increased. The relative mRNA of IL-4, IL-5, and IL-13 was decreased by AM. AM increased the proportion of CD4+ CD25+ Foxp3+ T cells in the spleen and nasal lymphoid tissue. In addition, AM could reduce the activity of NF-kB by inhibiting the mRNA expression and DNA binding activity of NF-κB p65. However, AM had no significant effect on mRNA of IκBα. Above all, AM could reduce the p-NF-κB p65 protein expression of nasal mucosa. Conclusions AM could reduce the secretion of inflammatory cytokines by increasing the level of CD4+ CD25+ Foxp3+ T cells and inhibiting the activation of the NF-κB.

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“…Der Bestandteil Astragalus (Huang qi) zeigte auch eine IFN-γ-aktivierende sowie eine IL-4-hemmende Wirkung bei Asthmatikern und könnte daher zu einer Verbesserung der Dysregulation der Th1/Th2-Balance bei Asthmatikern beitragen (Wang et al 2006).…”

Section: Anmerkung Der Autorinunclassified

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2020

Allergieprävention

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Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma

Abstract: AM could increase the expression of T-bet mRNA and Thl cytokines such as IFN-Y, and might reverse the Th2 predominant status in asthma patients.

Cited by 15 publications

References 22 publications

Astragalusmembranaceusmodulates Th1/2 immune balance and activates PPARγ in a murine asthma model

Astragalusmembranaceusmodulates Th1/2 immune balance and activates PPARγ in a murine asthma model

Effect of Astragalus membranaceus in Ovalbumin-Induced Allergic Rhinitis Mouse Model

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