Effects of astaxanthin on oxidative stress induced by Cu2+ in prostate cells

Abstract

Astaxanthin (AST), a carotenoid molecule extensively found in marine organisms and increasingly used as a dietary supplement, has been reported to have beneficial effects against oxidative stress. In the current paper, the effects of AST on viability of prostate cells were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay; cell apoptosis and intracellular reactive oxygen species (ROS) levels were determined by flow cytometry; the mitochondrial membrane potential (MMP) was measured by fluorospectrophotometer; and activities of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were evaluated by a detection kit. The results show that copper ion (Cu 2+ ) induced apoptosis, along with the accumulation of intracellular ROS and MDA, in both prostate cell lines (RWPE-1 and PC-3). AST treatments could decrease the MDA levels, increase MMP, and keep ROS stable in RWPE-1 cell line. An addition of AST decreased the SOD, GSH-Px, and CAT activities in PC-3 cell line treated with Cu 2+ , but had a contrary reaction in RWPE-1 cell lines. In conclusion, AST could contribute to protecting RWPE-1 cells against Cu 2+ -induced injuries but could cause damage to the antioxidant enzyme system in PC-3 cells.

The Role of Citrus Nobiletin on Oxidative Stress Levels and Superoxide Dismutase Activities in Metastatic Castration-Resistant Prostate Cancer

“…Oxidation of lipid peroxidation is one of the most widely reported markers of oxidative stress as a contributor to cancer and MDA is the most frequently studied of these markers. In one study, Meng et al (2017) showed that Cu 2+ -induced intracellular ROS accumulation and increased MDA levels with apoptosis reduced by astaxanthin treatment and this might have a protective effect against Cu 2+ -induced oxidative damage by reducing ROS and MDA levels. Similarly, our results revealed that NOB treatment decreased the MDA level in PC-3 cells compared to the control cells not treated with NOB.…”

SPC212 Human Mesothelioma Cell Underwent Apoptosis, Oxidative Stress, Morhological Deformation Following Astaxanthin Treatment

“…ROS production and antioxidant defense capability cause oxidative stress. Oxidative stress results in cell damage (Meng, Ni, Yu, Wang, & Shen, 2017). ASX has antioxidant, anti-in ammatory and immunomodulatory properties.…”

SPC212 Human Mesothelioma Cell Underwent Apoptosis, Oxidative Stress, Morhological Deformation Following Astaxanthin Treatment

“…ASX decreased ROS and thereby aborted intrinsic apoptosis by upregulating anti-apoptotic Bcl-2 expression and downregulating pro-apoptotic Bax expression (Wen et al, 2015 (Kavitha, Kowshik, Kishore, Baba, & Nagini, 2013). Meng et al reported ASX treatment is not a clear indicator of protective effects on apoptosis for RWPE-1 or PC-3 cells by Annexin V (Meng et al, 2017).…”