Effects of Aspirin on Embolization in an Arterial Model of Laser-Induced Thrombus Formation

Abstract

This model of arterial thrombosis induced by laser was used to evaluate the effect of aspirin (Aspegic®) on embolization. A partial occlusion was induced in small mesenteric arterioles (diameter 35-40 μm) with an Argon Laser. The laser induced the formation of a vessel wall lesion with damage of endothe-lial cells. Thrombus formed within seconds after the laser lesion and grew rapidly. Embolization began within the minute following the laser flash. Thrombus formation and embolization were repetitive phenomena. The duration of embolization was 6.50 ± 0.84 min in the control group. Then the thrombus became stable and partially obstructed the vessel lumen. The administration of aspirin at three doses (50, 100, 200 mg/kg) by intramuscular injection, 15 min before the laser injury, induced three different phenomena: (1) an increase of the number of laser injuries required for the thrombus formation; (2) a dose-dependent decrease in the duration of embolization, and (3) a dose-dependent decrease in the number of emboli. The highest dose injected induced the strongest reduction in the duration of embolization and the number of emboli.

Imaging of Structural Changes in Endothelial Cells and Thrombus Formation at the Site of FeCl₃-Induced Injuries in Mice Cremasteric Arteries

“…The inhibiting effects of aspirin in an animal model of thrombosis varied in the previous publication. Similar to the previous FeCl3-induced thrombus model 25) , but unlike arterial thrombus models induced by laser light or balloon injury 26,27) , the inhibiting effect of aspirin was only modest in our study. Similar results were reproduced in ex vivo perfusion experiments on immobilized collagen fibrils under controlled blood flow conditions.…”

Modifications Produced by Indomethacin and <i>L</i>-NAME in the Effect of Ultralow-Dose Aspirin on Platelet Activity in Portal Hypertension

“…Microscopic images were recorded through a video camera (DX L107, color camera CCD) and were monitored on a television screen (Trinitron color video monitor, PVM 144 2 QM, Sony, Paris, France). A schematic view of the apparatus has been described previously [13] . Arterioles with a diameter of 15-25 m were used.…”

Platelet Aggregation in Portal Hypertension and Its Modification by Ultra-Low Doses of Aspirin

“…Microscopic images were recorded through a video camera (DX L107, color camera CCD) and were monitored on a television screen (Trinitron color video monitor, PVM 144 2 QM, Sony, France). A schematic view of the apparatus used has been previously described [9] . Arterioles between 15 and 25 m in diameter were used.…”