Effects of ascorbic acid, phytic acid and tannic acid on iron bioavailability from reconstituted ferritin measured by an<i>in vitro</i>digestion–Caco-2 cell model

Jin, Fuxia; Frohman, Charles; Thannhauser, Theodore W.; Welch, Ross M.; Glahn, Raymond P.

doi:10.1017/s0007114508055621

Cited by 54 publications

(56 citation statements)

References 36 publications

(44 reference statements)

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“…The soluble iron form is later transported through the xylem to the cytoplasm of leaves, and transported by phloem to sink tissue (such as seeds) for storage (Kim and Guerinot, 2007). For legume crops, such as pea and lentil, approximately 90 % of the iron is stored in the ferritin form (Jin et al, 2009;Theil and Briat, 2004), and ferritin is stored in the embryo, not in the seed coat (Marentes and Grusak, 1998).…”

Section: Iron Utilization and Absorption In Plantsmentioning

confidence: 99%

“…Heme iron, typical of animal-based sources, has a relatively stable and higher bioavailability (Anderson and McLaren, 2012). Iron bioavailability of non-heme iron is affected significantly by promoters or inhibitors in the diet (Anderson and McLaren, 2012;Jin et al, 2009). AA, meat, fish and poultry proteins are the major promoters of iron bioavailability, while phytate, tannins and Ca are the main inhibitors (Anderson and McLaren, 2012;Bañuelos and Lin, 2008;Jin et al, 2009;Kalgaonkar and Lönnerdal, 2008).…”

Section: Iron Bioavailabilitymentioning

confidence: 99%

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Iron Bioavailability in Low Phytate Pea

et al. 2015

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Field pea (Pisum sativum L.) seeds have high nutritional value but also contain phytate which can inhibit the absorption and utilization of nutrients. Phytate is the main storage form of phosphorus in the seeds but chelates Fe, Zn and some other micronutrients and is not well digested by monogastrics. Peas with pigmented seed coats contain polyphenols which also have anti-nutritional properties. To increase the nutritional value of field pea seeds, two low phytate lines (1-150-81 and 1-2347-144) containing higher inorganic phosphorus concentration (IN-P) and lower phytate-phosphorus concentration (PA-P) than the normal phytate varieties were developed from CDC Bronco in previous research. The objectives of this research were 1) to determine the effect of genotype and environment on iron bioavailability in a set of five pea varieties differing in phytate concentration and iron concentration using in vitro digestion/Caco-2 cell culture bioassay; 2) to determine the effect of seed coats on iron bioavailability by testing whole seeds compared to dehulled seeds in varieties differing in seed coat pigmentation using in vitro digestion/Caco-2 cell culture bioassay; 3) to determine the inheritance of iron bioavailability in field pea by evaluating recombinant inbred lines differing in phytate concentration using in vitro digestion/Caco-2 cell culture bioassay; 4) to determine the effects of pea with the low phytate trait on body weight and hemoglobin concentration of chickens. Iron concentration (FECON) did not differ significantly between normal and low phytate varieties. Iron bioavailability (FEBIO) of the two low-phytate lines was 1.4 to 1.9 times higher than that of the three normal phytate varieties, and growing environment also had a significant effect on FEBIO. Peas with pigmented seed coats contained 7 times lower FEBIO than peas with non-pigmented seed coat. The removal of the seed coat increased the FEBIO in peas with pigmented seed coat 5 to 6 times. From previous research on PR-15 recombinant inbred lines (RILs) which were developed from a cross between low phytate line 1-2347-144 and a normal phytate variety CDC Meadow, it was found that PA-P was controlled by a single gene. FEBIO, in this study, was also found to follow a bimodal frequency distribution, characteristic of single gene iii control, and it was highly correlated with PA-P in the PR-15 lines. In vivo studies were used to evaluate iron absorption of chickens fed with low and normal phytate pea diets. The diets containing the low-phytate pea lines had no significant effect on chicken body weight and hemoglobin level, compared with the diets containing normal phytate pea varieties. An unexpected high FECON was discovered in the diets that was traced to the ingredients of limestone and dicalcium-phosphate which likely affected the experimental results.iv

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Section: Iron Utilization and Absorption In Plantsmentioning

confidence: 99%

Section: Iron Bioavailabilitymentioning

confidence: 99%

Iron Bioavailability in Low Phytate Pea

et al. 2015

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“…Exogenous phytase addition has been used to enhance the mineral bioavailability [107], and dephytinization by the addition of exogenous phytase in porridges of different cereal crops such as maize, oats rice, and wheat has been shown to improve iron bioavailability in human [108]. It has been demonstrated that phytic acid significantly reduces (about 86%) bioavailability of Fe from infant cereal diets in an in vitro digestion study in a Caco-2 cell model [109], and dephytinization markedly improved Fe and Zn bioavailability in these diets [110]. Park et al [111] reported a decrease of 20-40% in phytate content in a reaction time of 30-60 min by addition of alkaline phytase to whole-wheat bread.…”

Section: Beneficial Microbes For Human Healthmentioning

confidence: 99%

Beneficial microbiomes: Biodiversity and potential biotechnological applications for sustainable agriculture and human health

Yadav¹,

Kumar²,

Kumar³

et al. 2017

J App Biol Biotech

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The beneficial microbes plays an important role in medical, industrial, and agricultural processes. The precious microbes belong to different groups including archaea, bacteria, and fungi which can be sort out from different habitat such as extreme environments (acidic, alkaline, drought, pressure, salinity, and temperatures) and associated with plants (epiphytic, endophytic, and rhizospheric) and human. The beneficial microbes exhibited multifunctional plant growth promoting (PGP) attributes such as N 2 -fixation, solubilization of micronutrients (phosphorus, potassium and zinc), and production of siderophores, antagonistic substances, antibiotic, auxin, and gibberellins. These microbes could be applied as biofertilizers for native as well as crops growing at diverse extreme habitat. Microbes with PGP attributes of N 2 -fixation, P-, and K-solubilization could be used at a place of NPK chemical fertilizers. Agriculturally, important microbes with Fe-and Zn-solubilizing attributes can be used for biofortification of micronutrients in different cereal crops. The biofertilizers are an eco-friendly technology and bioresources for sustainable agriculture and human health. In general, the concentrations of micronutrient in different crops are not adequate for human nutrition in diets. Hence, consumption of such cereal-based diet may result in micronutrient malnutrition and related severe health complications. The biofortification approach is getting much attention to increase the availability of micronutrients, especially Fe and Zn in the major food crops. The beneficial microbes can be used as probiotic as functional foods for human health. Probiotics microbes such as Bifidobacterium, Lactobacillus, Methanobrevibacter, Methanosphaera, and Saccharomyces are increasingly being used as dietary supplements in functional food products. The microbes with beneficial properties could be utilized for sustainable agriculture and human health.

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“…There are many studies available in the scientific literature about iron absorption and regulation. Iron supplementation studies determine factors that affect iron absorption and oxidative damage (Beach et al, 2003;Casanueva and Viteri, 2003;Troost et al, 2003;Lonnerdal et al, 2006;Nagababu et al, 2008;Jin et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

“…E-mail: javierdc@ugr.es A variety of experimental models have been used in studies related to iron through the time (Latunde-Dada et al, 1998;Srigiridhar and Nair, 2000;Morgan and Oates, 2002;Troost et al, 2003;Nagababu et al, 2008;Quintero et al, 2008;Jin et al, 2009;Latunde-Dada, 2009). Nevertheless, in vitro systems or animal models have different iron metabolism behaviour when compared with humans (Latunde-Dada et al, 1998;Vaghefi et al, 2005;Patterson et al, 2008;Quintero et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Advantages and disadvantages of the animal models v. in vitro studies in iron metabolism: a review

García¹,

Díaz-Castro

2013

Animal

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Iron deficiency is the most common nutritional deficiency in the world. Special molecules have evolved for iron acquisition, transport and storage in soluble, nontoxic forms. Studies about the effects of iron on health are focused on iron metabolism or nutrition to prevent or treat iron deficiency and anemia. These studies are focused in two main aspects: (1) basic studies to elucidate iron metabolism and (2) nutritional studies to evaluate the efficacy of iron supplementation to prevent or treat iron deficiency and anemia. This paper reviews the advantages and disadvantages of the experimental models commonly used as well as the methods that are more used in studies related to iron. In vitro studies have used different parts of the gut. In vivo studies are done in humans and animals such as mice, rats, pigs and monkeys. Iron metabolism is a complex process that includes interactions at the systemic level. In vitro studies, despite physiological differences to humans, are useful to increase knowledge related to this essential micronutrient. Isotopic techniques are the most recommended in studies related to iron, but their high cost and required logistic, making them difficult to use. The depletion--repletion of hemoglobin is a method commonly used in animal studies. Three depletion--repletion techniques are mostly used: hemoglobin regeneration efficiency, relative biological values (RBV) and metabolic balance, which are official methods of the association of official analytical chemists. These techniques are well-validated to be used as studies related to iron and their results can be extrapolated to humans. Knowledge about the main advantages and disadvantages of the in vitro and animal models, and methods used in these studies, could increase confidence of researchers in the experimental results with less costs.

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Effects of ascorbic acid, phytic acid and tannic acid on iron bioavailability from reconstituted ferritin measured by anin vitrodigestion–Caco-2 cell model

Cited by 54 publications

References 36 publications

Iron Bioavailability in Low Phytate Pea

Iron Bioavailability in Low Phytate Pea

Beneficial microbiomes: Biodiversity and potential biotechnological applications for sustainable agriculture and human health

Advantages and disadvantages of the animal models v. in vitro studies in iron metabolism: a review

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