Effects of ascorbic acid on anxiety state and affect in a non-clinical sample

Moritz, Bettina; Schwarzbold, Marcelo Libório; Guarnieri, Ricardo; Diaz, Alexandre Paim; Rodrigues, Ana Lúcia S.; Dafre, Alcir Luiz

doi:10.21307/ane-2017-068

Cited by 15 publications

(7 citation statements)

References 76 publications

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“…Moreover, the mean heart rates were significantly different between vitamin C group and placebo control group (de Oliveira, de Souza, Motta, & Da-Silva, 2015). In another similar study, Moritz et al (2017) demonstrated that AA could affect anxiety level with a single dose oral of AA (1000 mg) or placebo in graduate students evaluated by the State–Trait Anxiety Inventory (STAI), and Visual Analogue Mood Scale (VAMS). The authors conclude that supplementation of AA reduces significantly from baseline anxiety scores in the STAI state-anxiety scale as well as VAMS anxiety subscale.…”

Section: Discussionmentioning

confidence: 96%

Effects of ascorbic acid combined to environmental enrichment on anxiety and memory.

Oliveira¹,

Souza²,

Carvalho³

et al. 2019

Psychology & Neuroscience

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Ascorbic acid (AA; vitamin C) is an important antioxidant that is directly involved in regulating physiological and psychological systems such as anxiety, memory, depression, stress, and emotional mood states. Environmental enrichment (EE) studies in animals have shown improvements in anxiety and memory as measured by behavioral experiments from living in improved settings. In the present study, the influences of AA and enriched environments were explored in 13-month-old rats, considered middle-aged, half of which were housed for up to 13 months in an enriched environment with a rich diversity of objects to explore, while the other half was maintained in a standard environment with only water and food, to understand better their independent and combined benefits to improving anxiety and memory. The rats were administered either a saline solution or AA (100 mg/kg, orally [p.o.]) 1 hr before being tested on the elevated plus maze (EPM) and the Morris water maze (MWM) behavioral tests. AA reduced anxiety-like behavior and improved spatial memory relative to saline-treated control rats. EE increased the number of entries to the open arms (anxiolytic-like behavior) and improved spatial memory retention. EE potentiated the anxiolytic-like effect of AA. These findings provide additional evidence that AA might play an important psychological therapeutic role as adjunctive psychopharmacotherapy in reducing levels of anxiety and memory deficits in human subjects.

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Section: Discussionmentioning

confidence: 96%

Effects of ascorbic acid combined to environmental enrichment on anxiety and memory.

Oliveira¹,

Souza²,

Carvalho³

et al. 2019

Psychology & Neuroscience

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“…However, it is also possible that changes in ber intake are re ective of other dietary changes that have shown to directly affect mood (omega 3, vitamin C, tryptophan, etc.) [107][108][109][110]. In this case, it could be that the other nutrients explain the relationship between diet and anxiety levels and not merely ber intake.…”

Section: Discussionmentioning

confidence: 97%

Association between the Distal Gut Microbiome and Anxiety in Highly Active Individuals

Marroquin¹,

Fletcher

Hwang

et al. 2020

Preprint

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Background: Traditional thinking is that physical activity benefits mental and physical health, however, excessive physical activity can increase anxiety, depression, and affect the gut microbiome. Considering the strong connection between the gut and the brain, the purpose of the present study was to evaluate the association between gut microbiota composition and anxiety as well as depression in highly active individuals. Methods: Participants included 55 young adults (ages 18-25, 51% males). All participants were highly physically active, as determined by 7 days of SenseWear monitoring. Anxiety and depression were measured with the Beck Anxiety and Depression Inventories. Alpha diversity, beta diversity, and microbial composition were evaluated via 16S rRNA gene sequencing using distal gut samples. Results: Greater anxiety was associated with both lower distal gut alpha diversity ( P < 0.05) and higher beta diversity (PERMANOVA test; R-squared: 0.17562, P = 0.027), which appeared stronger in males. Genus level taxonomic abundance analysis showed Prevotella relative abundance as higher in males with higher anxiety ( P = 0.03, q=0.06). However, adjusted linear regression analysis, controlling for fiber intake and sex nullified the association between Prevotella and anxiety. Additional analysis demonstrated a strong association between lower dietary fiber intake and higher anxiety scores (Est.= -0.48, SE= 0.20 , P = 0.021). Conclusion: In highly active individuals, specifically males, there is a strong relationship between the gut microbiome, fiber intake, and anxiety. These data suggest highly active males with anxiety may benefit from increased dietary fiber intake.

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“…In a recently published study from our laboratory the consumption of sodium benzoate at 125 mg/kg of feed was associated with an anxiogenic response in rodents [13]. The effect of dietary forti cation with ascorbic acid on anxiety has been studied severally [48][49][50][51].. In another study carried out in our laboratory, an anxiolytic effect was observed in animals that were fed ascorbic acid at 300 mg/kg of feed [40].…”

Section: Discussionmentioning

confidence: 99%

Graded effects of dry-feed added sodium benzoate/ascorbic acid combination on neurobehaviour, brain markers of oxidative stress, and markers of inflammation in mice

Olofinnade

Onaolapo

2023

Preprint

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Background:Controversies concerning the safety of sodium benzoate/ascorbic acid combination have remained grounds for continued scientific investigations. However, there is a dearth of scientific literature on its effect on the brain. This study investigated the effects dry-food added sodium benzoate/ascorbic acid combination on neurobehaviour, brain oxidative stress and inflammatory cytokines in mice. Methods: Adult male mice were assigned into ten groups of 10 mice each. The groups are: one group fed standard diet, three groups fed diet containing sodium benzoate (NaB) at 125 mg/kg with ascorbic acid (AA) at 100, 200 or 300 mg/kg of feed, another three groups fed NaB at 250 mg/kg with AA at 100, 200 or 300 mg/kg of feed respectively, and the last three groups fed NaB at 500 mg/kg with AA at 100, 200 or 300 mg/kg respectively. Behavioural tests were assessed following which animals were sacrificed and their brains homogenised for the assessment of biochemical parameters. Results: The result showed a decrease in body weight, self grooming, total antioxidant capacity and inflammatory cytokines, mixed response with food intake, locomotor activity, Y maze spatial working memory and anxiety related behaviours and an increase in rearing and radial arm maze spatial working memory. Conclusion: Dry food added NaB/AA altered behavioural, oxidative and inflammatory markers in mice. While not indicating outright deleterious effects at the concentrations used, the results suggest the need for further investigations into the effects of their combination, especially, when we consider their wide range of applications in the food industry.

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Effects of ascorbic acid on anxiety state and affect in a non-clinical sample

Cited by 15 publications

References 76 publications

Effects of ascorbic acid combined to environmental enrichment on anxiety and memory.

Effects of ascorbic acid combined to environmental enrichment on anxiety and memory.

Association between the Distal Gut Microbiome and Anxiety in Highly Active Individuals

Graded effects of dry-feed added sodium benzoate/ascorbic acid combination on neurobehaviour, brain markers of oxidative stress, and markers of inflammation in mice

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