Effects of aroclor 1254 on proteins of mouse liver: Application of two‐dimensional electrophoretic protein mapping

Abstract

Liver proteins of male C57BL/6T mice treated with 0, 50, or 250 mg/kg Aroclor 1 254 were analyzed by high-resolution two-dimensional (2-D) electrophoresis. The resulting patterns were processed using a computerized image analysis system and quantitative data selected for a total of 150 protein spots. On the basis of an analysis of liver proteins from five animals in each treatment group, we found 3 1 proteins that showed quantitative differences attributable to treatment with chlorinated hydrocarbons at a high level of statistical significance. One ofthe altered proteins appears to be Mitcon:2, a heat-shock sensitive mitochondria1 matrix polypeptide; another appears likely to be microsomal cytochrome b5. The results indicate that quantitative 2-D protein mapping may reveal much more detail regarding the in vivo effects of toxic xenobiotics than has previously been available, and thus allow a more informative approach to the testing of toxic compounds.

Analysis of scaling methods to minimize experimental variations in two‐dimensional electrophoresis quantitative data: Application to the comparison of maize inbred lines

“…This constant can be arbitrarily defined or calculated as the mean sum of the volume on all gels. This is the most commonly used method (linear scaling method [15,211).…”

“…Different ways to minimize experimental Variations by a proper treatment of the data have been investigated [7,15,20,211. In this study, we estimated the components of the experimental variation in a design involving 21 inbred maize lines.…”

Analytical and biological variances associated with proteomic studies of Medicago truncatula by two-dimensional polyacrylamide gel electrophoresis

“…The resolving power of this separation technology has found great utility in proteomic studies of plants [6][7][8][9][10][11], animals [12][13][14] and microorganisms [15,16]. Comparative analyses of 2-DE images yield quantitative information concerning changes in protein expression levels that can be used to understand gene function or to correlate cellular responses to biotic or abiotic stimuli [17,18]. Comparative analyses have been traditionally performed using human visualization; however, these comparisons can now be performed using commercial software programs in an automated or user-assisted manner [19][20][21].…”

Twenty years of two‐dimensional electrophoresis: Past, present and future

“…In studies of enzyme induction by the chlorinated hydrocarbon mixture Araclor 1254 in liver, the endoplasmic reticulum is usually isolated for analysis because the doctrine is that the interesting effects occur in this subcellular fraction. However, if one looks at the 2-DE protein pattern of whole liver from animals treated with this compound, most of the changes found (and not previously suspected) occur in the soluble and mitochondria1 fractions [44]. when liver of male and female mice are compared, more than one third of proteins differ significantly in abundance [45].…”